Description
This product is supplied as a ready-to-use peroxidase substrate containing 3,3',5,5'-tetramethylbenzidine (TMB) in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase in microwell applications (e.g. ELISA assays). For end-point assays, acid can be used to stop the reaction, yielding a yellow end product. Since this substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting