Japanese Encephalitis IgM ELISA Kit DEIA1978 from Creative Diagnostics

Product Specs
ItemJapanese Encephalitis IgM ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIA1978
Quantity96T
ApplicationsELISA
ReactivityHuman
Sample Typeserum
Detection TargetEncephaliti
SensitivitySerological Sensitivity: 31/31, or 100%
Components1. Coated Microtiter Strips for Human IgM: Strip holder in foil pouch, containing 96 polystyrene microtiter wells coated with antibody to human IgM in each well. Store at 2-8˚C until ready to use. The anti-human IgM coated wells are used to capture IgM antibodies from human samples.
2. Sample Dilution Buffer for IgM Type A: One bottle, 25 mL, for serum dilution prior to use in assay. Store at 2- 8˚C until ready to use. Note: If any precipitate is seen, vortex the tube very well to obtain a homogeneous solution before use.
3. JE Negative Control: One vial, 50 µL. The JE Negative Control will aid in monitoring the integrity of the kit. Store at 2-8˚C until ready to use. Quick spin the vial briefly before use to collect the content at the bottom.
4. JE IgM Positive Control: One vial, 50 µL. The JE IgM Positive Control will aid in monitoring the integrity of the kit. Store at 2-8˚C until ready to use. Quick spin the vial briefly before use to collect the content at the bottom.
5. Ready to Use JE Antigen (JERA) for IgM: One bottle, 5 mL of a pre-diluted JERA solution. Store at 2-8˚C until ready to use.
6. Ready to Use normal cell antigen (NCA) for JE IgM: One bottle, 5 mL of a prediluted NCA solution. Store at 2-8˚C until ready to use.
7. Ready to Use Enzyme Conjugate-HRP for JE IgM: One bottle, 9mL of a prediluted HRP conjugated flavivirus reactive monoclonal antibody (mAb). Store at 2- 8˚C until ready to use. Note: The conjugate should be kept in a light-protected bottle at all times as provided.
8. 10X Wash Buffer: One bottle, 120 mL of Wash Buffer to be used in all the washing steps of this procedure. Store at 2-8˚C until ready to use.
9. EnWash: One bottle, 20 mL of EnWash to be used in between the washing steps after the addition of enzyme conjugate-HRP of this procedure. Store at 2-8°C until ready to use.
10. Liquid TMB Substrate: One bottle, 12 mL of liquid substrate. Store at 2-8˚C until ready to use. Note: The substrate should be kept in a light -protected bottle at all times as provided.
11. Stop Solution: One bottle, 9 mL to be used to stop the reaction. Store at 2-8˚C until ready to use. Caution: strong acid, wear protective gloves, mask and safety glasses. Dispose of all materials according to safety rules and regulations

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Creative Diagnostics

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44-161-818-6441 (Europe)

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Japanese Encephalitis IgM ELISA Kit from Creative Diagnostics

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Japanese Encephalitis IgM ELISA Kit from Creative Diagnostics

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