Mitochondrial Membrane Potential Assay Kit (II) 13296S from Cell Signaling Technology

Product Specs
ItemMitochondrial Membrane Potential Assay Kit (II)
CompanyCell Signaling Technology
Price $356.00 Inquire
Catalog Number13296S
Quantity1 Kit
Research AreaScreening Technologies
ApplicationsIF-IC, FC-FP
ReactivityAll

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Development and Characterization of a Guar Gum Bionanocomposite Loaded with Biogenic Selenium Nanoparticles and Its Cytotoxic EvaluationGelsApril 30, 2026José Armando Hernández-Díaz, Ana Alejandra Arias-García, Alberto Gutiérrez-Becerra, Mauricio Comas-García, Milton Oswaldo Vázquez-Lepe, Orlando Hernández-Cristóbal, Soledad García-Morales, Moisés Martínez-Velázquez, Zaira Yunuen García-CarvajalMitochondrial membrane potential was assessed using the Mitochondrial Membrane Potential Assay Kit II (#13296, Cell Signaling Technology, Danvers, MA, USA) with TMRE and CCCP as a mitochondrial uncoupler. Cells

(2) Activation of cGAS-STING Pathway by DAI-Triggered Ferroptosis in CRC Cells Reprograms TAMs Balance to Promote Anti-Tumor ImmunityCancer scienceDecember 1, 2025Cheng P, Yang Q, Zhang X, Wang Q, Zhong B.Potential (MMP) The MMP changes were measured using the MMP Assay Kit (II) (Cell Signaling Technology, #13296, USA) according to the manufacturer's guidelines. 2.7 Transmission Electron Microscopy (TEM) The mitochondrial

(3) Exploiting dysregulated iron homeostasis to eradicate persistent high-grade serous ovarian cancerCell Death DiscoverySeptember 25, 2025Carmelo Cerra, Madeleine R. C. Tancock, Niko Thio, Ada Koo, AnnRann Wong, Karla J. Cowley, Swati Varshney, Madelynne O. Willis, Kaylene J. Simpson, Australian Ovarian Cancer Study Group, et al.measured using mitochondrial membrane potential assay kit (Cell Signaling Technology, Danvers, MA, USA #13296). Tetramethylrhodamine (TMRE) (Invitrogen, Waltham, MA #T669) at 0.2 µM was added to cell culture media

(4) The Responsiveness of Breast Cancer Cells to Varied Levels of Vitamin B12, Cisplatin, and G-CSFInternational Journal of Molecular SciencesSeptember 18, 2025Volkan Aslan, Duygu Deniz Usta, Atiye Seda Yar Sağlam, Ahmet Özet, Osman Sütcüoglu, Kürşat Dikmen, Nuriye ÖzdemirMitochondrial Membrane Potential Assay Kit II was purchased from Cell Signaling Technology (Danvers, MA, USA) (CST, 13296), and a BCA Protein Assay Kit was obtained from Thermo Scientific (Waltham, MA, USA) (Ref: 23227). All

(5) Widespread stable noncanonical peptides identified by integrated analyses of ribosome profiling and ORF featuresNat CommunMarch 2, 2024Haiwang Yang, Qianru Li, Emily K. Stroup, Sheng Wang, Zhe Jigrown to 80% confluency the next day, CCCP (positive control of mitochondrial membrane potential loss; 13296, Cell Signaling Technology) was added to the control wells to a 50 µM final concentration. Cells were

(6) Proteomic analysis of exosomes secreted during the epithelial-mesenchymal transition and potential biomarkers of mesenchymal high-grade serous ovarian carcinomaJournal of ovarian researchNovember 29, 2023Ferreira GA, Thomé CH, Izumi C, Grassi ML, Lanfredi GP, Smolka M, Faça VM, Candido Dos Reis FJ.mitochondrial membrane potential was conducted using intracellular tetramethylrhodamine ethyl ester perchlorate (TMRE) (#13296, Cell Signaling), following the manufacturer’s instructions. To establish control, cell lines were incubated

(7) IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2iScienceAugust 18, 2023Lan Y, Yang T, Yue Q, Wang Z, Zhong X, Luo X, Zuo B, Zhang M, Zeng T, Liu B, et al.using the JC-1 Assay Kit (Beyotime, C2006, China) and the TMRE Assay Kit (Cell Signaling Technology, #13296, MA, USA). According to the manufacturer’s protocols, Cells fixed on confocal dishes were stained with

(8) Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein responseInternational journal of biological sciencesJanuary 1, 2023Cai C, Li Z, Zheng Z, Guo Z, Li Q, Deng S, Shi N, Ou Q, Zhou H, Guo Z, et al.health, was measured in this study 43. To do so, we used a Mitochondrial Membrane Potential Assay Kit (13296, Cell Signaling Technology, Danvers, MA, USA). This kit utilizes JC-1 staining, a fluorescent dye that

(9) PBDEs Found in House Dust Impact Human Lung Epithelial Cell HomeostasisToxicsFebruary 19, 2022Antonio Zandona, Karla Jagić, Marija Dvoršćak, Josip Madunić, Darija Klinčić, Maja Katalinićethyl ester perchlorate (TMRE) from the assay kit (Mitochondrial Membrane Potential Assay Kit (II), #13296, Cell Signaling Technology Europe, Leiden, The Netherlands). Namely, as described in our previous study

(10) Substrate Reduction Therapy Reverses Mitochondrial, mTOR, and Autophagy Alterations in a Cell Model of Gaucher DiseaseCellsSeptember 2, 2021Peng Y, Liou B, Lin Y, Fannin V, Zhang W, Feldman RA, Setchell KDR, Grabowski GA, Sun Y.medium was replaced with fresh neurobasal medium with 200 nM tetramethylrhodamine, ethyl ester (TMRE, 13296S, CST) added to each well and incubated at 37 °C for 20 min. For the control group, 50 µM carbonyl cyanide

(11) Aurora kinase A inhibition reverses the Warburg effect and elicits unique metabolic vulnerabilities in glioblastomaNature communicationsSeptember 1, 2021Nguyen TTT, Shang E, Shu C, Kim S, Mela A, Humala N, Mahajan A, Yang HW, Akman HO, Quinzii CM, et al.ethyl ester perchlorate) staining was performed according to the manufacturer’s instructions (CST, 13296S). Signal was detected on a LSRII flow cytometry (Becton–Dickinson, New Jersey) and the data were analyzed

(12) HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytesMolecular medicine reportsAugust 1, 2021Pang Y, Zhu Z, Wen Z, Lu J, Lin H, Tang M, Xu Z, Lu J.mitochondrial membrane potential was performed using a mitochondrial membrane potential assay kit (cat. no. 13296S; Cell Signaling Technologies, Inc.) according to the manufacturer's instructions. Flow cytometry for

(13) Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activationRedox biologyMay 1, 2021Sun J, Zhou C, Zhao Y, Zhang X, Chen W, Zhou Q, Hu B, Gao D, Raatz L, Wang Z, et al.potential (ΔΨm) were measured as previously described [43]. 1 × 106 cells were incubated with 200 nM TMRE (#13296, Cell signaling, USA) for 15 min at 37 °C. Cells were washed 3 times with PBS, harvested and suspended

(14) Epigenetic Targeting of Mcl-1 Is Synthetically Lethal with Bcl-xL/Bcl-2 Inhibition in Model Systems of GlioblastomaCancersAugust 1, 2020Shang E, Nguyen TTT, Shu C, Westhoff MA, Karpel-Massler G, Siegelin MD.USA), CST 4087S) to detect the subG1 fraction. TMRE staining (Mitochondrial Membrane Potential kit (CST 13296S)) was conducted to detect mitochondrial membrane potential. Collected data was analyzed by FlowJo software

(15) Sensing of ATP via the Purinergic Receptor P2RX7 Promotes CD8⁺ Trm Cell Generation by Enhancing Their Sensitivity to the Cytokine TGF-βImmunityJuly 14, 2020Borges da Silva H, Peng C, Wang H, Wanhainen KM, Ma C, Lopez S, Khoruts A, Zhang N, Jameson SC.M22426 Mitochondrial Membrane Potential Assay Kit (II) (TMRE),Cell Signaling Technologies,Cat no. 13296S Treg-Protector (anti-ARTC2 Nanobody) [S+16a],BioLegend,"Clone S+16a, Cat no. 149802; RRID:AB_2565494" ,, "Chemicals,

(16) Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model SystemsCellsJuly 10, 2020Nguyen TTT, Zhang Y, Shang E, Shu C, Quinzii CM, Westhoff MA, Karpel-Massler G, Siegelin MD.USA), CST 4087S). To detect mitochondrial membrane potential, Mitochondrial Membrane Potential kit (CST 13296S) was used. The FlowJo software (version 10.6.2; Tree Star, Ashland, OR, USA) was employed for the entire

(17) HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma modelsThe Journal of clinical investigationJuly 1, 2020Nguyen TTT, Zhang Y, Shang E, Shu C, Torrini C, Zhao J, Bianchetti E, Mela A, Humala N, Mahajan A, et al.tetramethylrhodamine, ethyl ester (TMRE) (Mitochondrial Membrane Potential Kit, Cell Signaling Technology [CST], 13296S). The data were analyzed with FlowJo software, version 8.7.1 (Tree Star). In addition, mitochondria

(18) Integrated analysis of the molecular pathogenesis of FDXR-associated diseaseCell Death & DiseaseJune 4, 2020Jesse D. Slone et al.Vermont, United States), with excitation at 550 nm and emission at 580 nm (Cell Signaling Technology, #13296). Anterograde axonal transport assay in mouse retinal ganglion cells (RGCs) Seven-month-old B6;129

(19) TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS LevelsCancersMay 22, 2020Yang W, Shin HY, Cho H, Chung JY, Lee EJ, Kim JH, Kang ES.mitochondrial membrane potential assay was performed using the Membrane Potential Assay Kit (II) (#13296S, Cell Signaling Technology, Danvers, MA, USA) and MitoTracker Green FM (Thermo Fisher Scientific, Rockford,

(20) D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degenerationSignal transduction and targeted therapyMarch 4, 2020Wang B, Wang L, Gu S, Yu Y, Huang H, Mo K, Xu H, Zeng F, Xiao Y, Peng L, et al.Mitochondrial membrane potential assay MMP was measured with a Mitochondrial Membrane Potential Assay Kit (CST, #13296S). Briefly, after culture in conditioned medium, cells were rinsed and washed 3 times with phosphate-buffered

Be the First to Write a Review!

Mitochondrial Membrane Potential Assay Kit (II) from Cell Signaling Technology

Description

Cell Signaling Technology

Citations (23)

Reviews

Mitochondrial Membrane Potential Assay Kit (II) from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map