Fig 1: The preventive effects of OABL on oxidative stress-induced PC12 neuronal death(A) Reaction of OABL and GSH in PBS. OABL (1 mM) and GSH (5 mM) were incubated in a solution of PBS: DMSO = 4: 1 at 37 °C for 2 h, and the reaction progress was monitored by HPLC-MS (see Fig. S2 in Supplementary data); (B) detection of GSH in live cells. PC12 cells were treated by various concentrations of OABL for 2 h or 24 h. The GSH level was measured according to a cellular GSH detection assay kit (#13859, CST) with the fluorescent intensity at an excitation wavelength of 380 nm and an emission wavelength of 460 nm using a SpectraMax M5 plate reader; (C) neurotoxic effect of OABL against PC12 cells. Cells were incubated with vehicle and OABL (1, 5, 10, and 20 μM) for 24 h; (D–G) oxidative damage was induced by H2O2, 6-OHDA, oxytosis was induced by glutamate, and ferroptosis was induced with RSL3 in PC12 cells. PC12 cells were incubated with vehicle (0.1%, DMSO) and OABL (1, 5, and 10 μM) for 24 h, and then exposed to various inducers for 24 h. The cell viability was assessed by MTT assay; inhibition effects of OABL (D) in 0.6 mM H2O2-induced PC12 cells; (E) in 1.0 mM 6-OHDA-induced PC12 cells; (F) in 100 mM glutamate-induced PC12 cells; (G) in 10 μM RSL3-induced PC12 cells. The statistical analysis was performed via student's t-test, and the data are presented as the means ± SEM from three or five independent experiments, each with 4–6 duplicates. (##) p < 0.01 as compared to control (DMSO); (*) p < 0.05 and (**) p < 0.01 vs. the inducers-treated group.