Description
Trichothecenes are a very large family of chemically related mycotoxins produced by various species of Fusarium, Myrothecium, Trichoderma, Trichothecium, Cephalosporium, Verticimonosporium, and Stachybotrys. Trichothecenes belong to sesquiterpene compounds. The most important structural features causing the biological activities of trichothecenes are: the 12,13-epoxy ring, the presence of hydroxyl or acetyl groups at appropriate positions on the trichothecene nucleus and the structure and position of the side-chain. They are produced on many different grains like wheat, oats or maize by various Fusarium species such as F. graminearum, F. sporotrichioides, F. poae and F. equiseti.
Principle of the Assay: This assay is based on the competitive enzyme immunoassay for the detection of T-2 in the sample. The coupling antigens are pre-coated on the micro-well stripes. The T-2 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-T2 antibodies. T-2er the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the T-2 in it. This value is compared to the standard curve and the T-2 concentration is subsequently obtained