Description
Intended Uses: This CYS-CELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCYS-Cin the sample, thisCYS-CELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCYS-Cconcentration. The concentration ofCYS-Cin the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This CYS-Cenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCPP.HS. Standards or samples are then added to the microtiter plate wells andCYS-Cif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCYS-Cpresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCYS-Care added to each well to "sandwich" theCYS-Cimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCYS-Cand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm