Description
Principle of the Assay: HSP 40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP 40 antibody and an HSP 40-HRP conjugate. The assay sample and buffer are incubated together with HSP 40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP 40 concentration since HSP 40 from samples and HSP 40-HRP conjugate compete for the anti-HSP 40 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP 40 from the sample, fewer sites are left to bind HSP 40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP 40 concentration in each sample is interpolated from this standard curve