Fig 1: Orthotopic tumor triggers perigonadal fat loss in high‐fat diet (HFD)‐fed mice and modulates lipid metabolism in adipose tissues. Mice fed a HFD from 5 weeks of age were orthotopically injected with murine‐derived mPKC1 pancreatic cancer cells at 15 weeks of age and killed at 18 weeks of age (sham, n = 4; orthotopic injection [ortho], n = 9). (A) Change in bodyweight (BW) of the HFD‐fed mice with and without orthotopic injection from 15 weeks (15w) of age to 18w of age. (B) Perigonadal fat weight per bodyweight of the HFD‐fed mice with and without orthotopic injection at 18 weeks of age. (C) Real‐time PCR undertaken on perigonadal fat of the HFD‐fed mice with and without orthotopic injection (Mann–Whitney U‐test). (D) Serum ELISAs of nonesterified fatty acids (NEFA) in mice with and without orthotopic injection. (E) Real‐time PCR of pentraxin 3 (PTX3), Srpx, and Sfrp2 extracted from perigonadal fat of the mice with and without orthotopic injection. (F) Serum ELISAs of PTX3 and Srpx in the mice with and without orthotopic injection. (G) Pearson correlation coefficient of serum PTX3 and mRNA expression of PTX3 in perigonadal fat. (H) Pearson correlation coefficient of PTX3 and perigonadal fat weight per BW, tumor weight, BW at 18 weeks, change in BW from 15 weeks to 18 weeks of age, and NEFAs. Error bars, mean ± SD; bar only, median. *p < 0.05. A.U., arbitrary units; ns, not significant.