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NanoBRET PPI Flexi Starter System
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NanoBRET PPI Flexi Starter System
NanoBRET PPI Flexi Starter System
NanoBRET PPI Flexi Starter System from Promega
Be the first to write a review!
Citations:
(6)
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NanoBRET PPI Flexi Starter System
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Description
Protein:Protein Interaction (PPI) Assays use NanoLuc Luciferase and HaloTag protein fused to target proteins of interest to enable sensitive, reproducible detection of protein interactions in the natural cellular environment
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NanoBRET PPI Flexi Starter System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
N1821
Quantity
1 each
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (6)
(1)
ACAT1‐Mediated ME2 Acetylation Drives Chemoresistance in Ovarian Cancer by Linking Glutaminolysis to Lactate Production
Advanced Science
February 14, 2025
Cuimiao Zheng, Hao Tan, Gang Niu, Xi Huang, Jingyi Lu, Siqi Chen, Haoyuan Li, Jiayu Zhu, Zhou Zhou, Manman Xu,
et al.
then harvested to
measure
the bioluminescence according to the manufacturer's instructions (catalog
N1821
;
Promega
). In Vivo Study Animal studies were performed according to protocols reviewed and approved
(2)
A novel class of inhibitors that disrupts the stability of integrin heterodimers identified by CRISPR-tiling-instructed genetic screens
Nature structural & molecular biology
March 1, 2024
Mattson NM, Chan AKN, Miyashita K, Mukhaleva E, Chang WH, Yang L, Ma N, Wang Y, Pokharel SP, Li M,
et al
.
(HG11269 for
ITGAV
and HG10779 for
ITGB5
, Sino Biological) into the NanoBRET HaloTag and NanoLuc plasmids (
N1821
,
Promega
), respectively. Alanine substitution of the
ITGAV
β-propeller HIP (Fig. 4d,f;
mutagenesis
primers
(3)
ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models
The Journal of clinical investigation
January 17, 2023
Li J, Lu J, Xu M, Yang S, Yu T, Zheng C, Huang X, Pan Y, Chen Y, Long J,
et al
.
then harvested to
measure
the bioluminescence according to the manufacturer’s instructions (catalog
N1821
;
Promega
). In vivo studies. For all animal studies, animals were randomly chosen, and no statistical
(4)
MARK2 phosphorylates eIF2α in response to proteotoxic stress
PLoS biology
March 1, 2021
Lu YN, Kavianpour S, Zhang T, Zhang X, Nguyen D, Thombre R, He L, Wang J.
values were calculated using equations from the manufacturer’s protocol (
Promega
, NanoBRET PPI Systems,
N1821
).
Mouse
and
human
tissues The SOD1 transgenic
mice
used in this study have been previously characterized:
(5)
Small-molecule-induced polymerization triggers degradation of BCL6
Nature
December 1, 2020
Słabicki M, Yoon H, Koeppel J, Nitsch L, Roy Burman SS, Di Genua C, Donovan KA, Sperling AS, Hunkeler M, Tsai JM,
et al
.
resonance energy transfer (BRET) experiments were performed using a NanoBRET PPI starter kit (
Promega
N1821
) according to the manufacturer’s instructions and as previously described45.
In vitro
ubiquitination
In vitro
(6)
The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K
Nature
September 1, 2020
Słabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, Donovan KA, Sievers QL, Koeppel J, Suchyta D,
et al
.
analyses Bioluminescence resonance energy transfer (BRET) experiments were using a NanoBRET PPI starter kit (
Promega
N1821
) according to the manufacturer’s instructions and as previously described51. Drug
sensitivity
assays
HEK293TCas9
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NanoBRET PPI Flexi Starter System from Promega
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