Description
Description: The mouse monoclonal antibody isotyping test kit shows no cross-reactivity with bovine IgG (
Test Principle: The assay principle is based on anti-mouse kappa and anti-mouse lambda antibodies coupled onto coloured micro particles and equally reactive to any mouse monoclonal antibody regardless of its isotype. The isotyping strip has immobilized bands of goat anti-mouse antibodies corresponding to each of the common mouse antibody isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA) and to the kappa and lambda light chains. Both sides of the strip bear a positive flow control band, which indicates that the antibody-coated coloured micro particles have migrated through the strip. By using these two components, a mouse monoclonal antibody can be screened for isotype by simply diluting the antibody sample, pipetting the diluted sample into the development tube where it forms a complex with the antibody coated micro particles, and inserting the strip. This complex flows through the strip until it is bound by the immobilized goat anti-mouse antibody specific for the monoclonal's isotype and its light chain. In approximately 5-10 minutes, the micro particle complex will aggregate as blue bands in the two sections corresponding to the monoclonal antibody's isotype and its light chain. Development of the strip is complete when the positive flow control band on each side of the test strip turns blue