AMP-Glo Assay V5011 from Promega

Product Specs
ItemAMP-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5011
Quantity1,000 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) TRIM24 Regulates Adaptation to Glucose Deprivation in Association with Aspartate Accumulation and Impaired AMPK SignalingCurr Issues Mol BiolApril 14, 2026Xiaochen Yu, Duopeng An, Dadui Ren, Peng He, Yunkai Yang, Nanye Chen, Rui Wang, Shan Wu, Jun Feng, Meiqing Fengmeasured using CellTiter-GloTM ATP Assay (Promega, Madison, WI, USA, G7570) and AMP-GloTM Assay (Promega, V5011), respectively, according to the manufacturers’ instructions. Cells cultured under control or glucose-free

(2) Adenosine metabolic clearance maintains liver homeostasis by licensing arginine methylation of RIPK1J Exp MedJanuary 5, 2026Ran Liu, Gengqiao Wang, Zhengting Jiang, Tianhao Zou, Chuanzheng Wang, Weimin Wang, Mao Cai, Shuhua Zhang, Guoliang Wang, Huan Cao, et al.(MET-5151-C; Cell Biolabs) was utilized. For measurement of AMP, ADP, and ATP concentrations, AMP-Glo assay (V5011; Promega), ADP-Glo assay (V6930; Promega), and CellTiter-Glo assay (G7570; Promega) were utilized. All

(3) Macrodomain ADP-ribose binding but not ADP-ribosylhydrolase activity is critical for chikungunya virus infection of Aedes mosquitoesbioRxivOctober 13, 2025Eugenia S. Bardossy, Lena Bergmann, Annabelle Henrion-Lacritick, Jared Nigg, Galen J. Correy, Alan Ashworth, James S. Fraser, Maria-Carla Salehphosphodiesterase to AMP, which was detected using the commercially available AMP-Glo kit (Promega, V5011). Reactions were prepared to a final volume of 8 μl in assay buffer (20 mM HEPES pH 7.5, 100 mM NaCl,

(4) A CD147-targeted small-molecule inhibitor potentiates gemcitabine efficacy by triggering ferroptosis in pancreatic ductal adenocarcinomaCell reports. MedicineAugust 19, 2025Li J, Ma C, Cao P, Guo W, Wang P, Yang Y, Ding B, Yin F, Li Z, Wang Y, et al.(S0208S), MDA (S0131), ATP (S0027), and GSH/GSSG (S0053) Assay Kits from Beyotime, and AMP Assay Kit (V5011) from Promega. RNA sequencing Total RNA was extracted using TRIzol reagent (Invitrogen) following

(5) A highland-adaptation variant near MCUR1 reduces its transcription and attenuates erythrogenesis in TibetansCell GenomMarch 12, 2025Ping J, Liu X, Lu Y, Quan C, Fan P, Lu H, Li Q, Wang C, Zhang Z, Liu M, et al.kit (#G7570; Promega, WI, USA). AMP levels were measured using the AMP-Glo luminescent assay kit (#V5011; Promega, WI, USA). Luminescence was measured using a microplate reader (Infinite M1000 PRO; Tecan,

(6) Enpp1 ameliorates MAFLD by regulating hepatocyte lipid metabolism through the AMPK/PPARα signaling pathwayCell BiosciFebruary 19, 2025Xiaohui Liu, Shuai Chen, Xing Liu, Xianxian Wu, Xiaoliang Jiang, Yuhan Li, Zhiwei Yangreported methods [16]. Then the concentration of AMP and ATP was measured with corresponding assay kits (#V5011, Promega and #S0026, Solarbio) according to the manufacturer’s protocol. Statistical analysis The

(7) Co‐Translational Deposition of N 6‐Acetyl‐L‐Lysine in Nascent Proteins Contributes to the Acetylome in Mammalian CellsAdvanced ScienceJanuary 1, 2025Dingyuan Guo, Nan Li, Xiaoyan Zhang, Runxin Zhou, Jie He, Xiao‐Ping Ding, Weixing Yu, Fuqiang Tong, Sibi Yin, Yu Wang, et al.mutants were determined by measuring AMP production and ATP consumption with AMP‐Glo Assay Kit (Cat#V5011, Promega, Madison, USA) and ATP Bioluminescent Assay Kit (Cat#FLAA, Sigma‐Aldrich, Shanghai, China)

(8) A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cellsCellFebruary 29, 2024Tieu V, Sotillo E, Bjelajac JR, Chen C, Malipatlolla M, Guerrero JA, Xu P, Quinn PJ, Fisher C, Klysz D, et al.commercial assays Adenosine Assay Kit (Fluorometric),Abcam,Cat#ab211094 AMP-Glo Assay,Promega,Cat#V5011 CellTiter-Glo 2.0 Cell Viability Assay,Promega,Cat#G9241 Cyto-Fast Fix/Perm Buffer Set,Biolegend,Cat#426803 DNeasy

(9) A method for measurement of human asparagine synthetase (ASNS) activity and application to ASNS protein variants associated with ASNS deficiencyBiology Methods and ProtocolsOctober 25, 2023Mario C Chang, Stephen J Staklinski, Matthew E Merritt, Michael S Kilbergwas assessed by measuring the production of AMP with a commercially available luminescence assay kit (V5011, Promega), and the workflow is detailed in Fig. 1A and B. Assay preparation involved thawing an aliquot

(10) Hepatitis C virus RNA is 5’ capped with flavin adenine dinucleotide (FAD)NatureJuly 27, 2023Anna V. Sherwood, Lizandro R. Rivera-Rangel, Line A. Ryberg, Helena S. Larsen, Klara M. Anker, Rui Costa, Cathrine B. Vågbø, Eva Jakljevič, Long V. Pham, Carlota Fernandez-Antunez, et al.specificity of AtNUDX23, NudC and Rpp (Lucigen, RP8092H) were determined with AMP-Glo assay (Promega, V5011) by measuring concentration of AMP generated by these proteins in the presence of different substrates

(11) A single inactivating amino acid change in the SARS-CoV-2 NSP3 Mac1 domain attenuates viral replication and pathogenesis in vivobioRxivMay 13, 2023Taha Y. Taha, Rahul K. Suryawanshi, Irene P. Chen, Galen J. Correy, Patrick C. O’Leary, Manasi P. Jogalekar, Maria McCavitt-Malvido, Morgan E. Diolaiti, Gabriella R. Kimmerly, Chia-Lin Tsou, et al.hydrolyzed by the NUDT5 phosphodiesterase to AMP, which is detected using the AMP-Glo assay kit (Promega, V5011). The activity of the recombinantly expressed NUDT5 with ADP-ribose was determined by serially diluting

(12) Characterizing asparagine synthetase deficiency variants in lymphoblastoid cell linesJIMD ReportsMarch 1, 2023Stephen J. Staklinski, Mario C. Chang, Rebecca C. Ahrens‐Nicklas, Shagun Kaur, Arianna K. Stefanatos, Elizabeth E. Dudenhausen, Matthew E. Merritt, Michael S. Kilbergdetection to monitor ASNS enzyme activity As described previously, 24 the AMP‐Glo assay kit (Promega no. V5011) was used to measure the AMP produced by purified WT or H205P ASNS protein per the manufacturer's protocol.

(13) Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1Frontiers in pharmacologyJanuary 1, 2023Ren G, Ding YW, Wang LL, Jiang JD.Kit (A22066) were purchased from Thermo Fisher Scientific Inc. (Shanghai, China). AMP-Glo™ Assay kit (V5011) was purchased from Promega Biotech Co., Ltd. (Beijing, China). The rabbit and mouse antibodies against

(14) Analysis of Enzyme Activity and Cellular Function for the N80S and S480F Asparagine Synthetase Variants Expressed in a Child with Asparagine Synthetase DeficiencyInternational Journal of Molecular SciencesDecember 29, 2022Stephen J. Staklinski, Sarah Snanoudj, Anne-Marie Guerrot, Catherine Vanhulle, François Lecoquierre, Soumeya Bekri, Michael S. Kilbergstored at −80 °C and thawed only once. 4.8. AMP-Based ASNS Enzyme Assay The Promega AMP-Glo Assay (#V5011) kit was used to measure AMP production by purified ASNS protein. In brief, a 100 ng aliquot of ASNS

(15) Cellular and molecular characterization of two novel asparagine synthetase gene mutations linked to asparagine synthetase deficiencyThe Journal of biological chemistrySeptember 1, 2022Staklinski SJ, Chang MC, Yu F, Collins Ruff K, Franz DN, Qian Z, Bloom LB, Merritt ME, McKenna R, Kilberg MS.at −80 °C until use. Aliquots were only thawed once. ASNS enzyme assay The Promega AMP-Glo Assay (#V5011) was adapted to detect the AMP produced by purified WT or variant ASNS proteins. A 100 ng aliquot of

(16) Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responsesCell reportsApril 19, 2022Saxena V, Piao W, Li L, Paluskievicz C, Xiong Y, Simon T, Lakhan R, Brinkman CC, Walden S, Hippen KL, et al.N/A EZ DNA Methylation-Direct™ Kit,Zymo Research,Cat# D5020; RRID: N/A AMP-Glo assay,Promega,Cat# V5011; RRID: N/A ELISA MAX™ Deluxe Set Mouse IL-6,BioLegend,Cat# 431104; RRID: N/A ELISA MAX™ Deluxe Set

(17) CD73-Mediated Immunosuppression Is Linked to a Specific Fibroblast Population That Paves the Way for New Therapy in Breast CancerCancersNovember 23, 2021Magagna I, Gourdin N, Kieffer Y, Licaj M, Mhaidly R, Andre P, Morel A, Vincent-Salomon A, Paturel C, Mechta-Grigoriou F.CAF-S1 primary cell lines was assessed using AMP GLOTM luminescence assay (Promega, Madison, WI, USA, #V5011) allowing the measurement of exogenous AMP hydrolysis. Harvested CAF-S1 were suspended in supplemented

(18) Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammationImmunityAugust 10, 2021Medina CB, Chiu YH, Stremska ME, Lucas CD, Poon I, Tung KS, Elliott MR, Desai B, Lorenz UM, Bayliss DA, et al.,Miltenyi Biotec,Cat# 130-091-041 CellTiter-Glo Luminescent Assay,Promega,Cat# G7570 AMP-Glo Assay,Promega,Cat# V5011 Adenosine Assay Kit,Abcam,Cat# ab211094 Lipofectamine 2.0,Thermo Fisher,Cat# 11668027 Deposited data,Deposited

(19) A Luminescence‐Based Coupled Enzyme Assay Enables High‐Throughput Quantification of the Bacterial Second Messenger 3’3’‐Cyclic‐Di‐AMPChemBioChemMarch 16, 2021Shivam A. Zaver, Alex J. Pollock, Vishant M. Boradia, Joshua J. Woodwardobtained from Invivogen (cat. no. tlrl-nacda and cat. no. tlrl-nacga, respectively). AMP-Glo™ (cat. no. V5011), Cell Titer Glo® (cat. no. G7570), and Kinase Glo® (cat. no. V6712) reagents were obtained from Promega Corporation

(20) Anti-Na(+)/K(+)-ATPase immunotherapy ameliorates α-synuclein pathology through activation of Na(+)/K(+)-ATPase α1-dependent autophagyScience advancesJanuary 1, 2021Cao L, Xiong S, Wu Z, Ding L, Zhou Y, Sun H, Zhu M, Lee WT, Nie X, Bian JS.S2. Measurement of AMP and ATP levels Cellular AMP and ATP levels were measured using an AMP kit (Promega, V5011) and an ATP kit (Promega, G9242) according to the manufacturer’s instructions, respectively. The ratio

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AMP-Glo Assay

AMP-Glo Assay from Promega

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AMP-Glo Assay from Promega

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