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CellTox Green Cytotoxicity Assay
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CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay from Promega
Be the first to write a review!
Citations:
(20)
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CellTox Green Cytotoxicity Assay
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Description
The CellTox Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTox Green Cytotoxicity Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8743
Quantity
100ml
Molecular Weight
~103kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (20)
(1)
Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathways
Redox Report
December 31, 2026
Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan Huang
concentrations of tramadol. Cytotoxicity was assessed using the
CellTox™ Green Cytotoxicity Assay
(catalog no.:
G8743
;
Promega
, Madison, WI, USA), whereas cell viability was measured using the
CellTiter-Glo® 2.0 Assay
(2)
Limited response of primary nasal epithelial cells to Bordetella pertussis infection
Microbiology Spectrum
September 2, 2025
Martin Zmuda, Ivana Malcova, Barbora Pravdova, Ondrej Cerny, Denisa Vondrova, Jana Kamanova
changes in
cell membrane
integrity using the fluorescent DNA binding dye CellTox Green (
Promega
, Cat. #
G8743
), as described previously (99). In brief, 2 × 104 of
HeLa cells
per well were seeded in a 96-well black/clear
(3)
Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems
Front Immunol
August 7, 2025
Rupsa Datta, Veronika Miskolci, Gina M. Gallego-López, Emily Britt, Amani Gillette, Aleksandr Kralovec, Morgan A. Giese, Tongcheng Qian, James Votava, Wenxuan Zhao,
et al.
curves. Cytotoxicity assay A cytotoxicity
assay
was performed using CellTox™ Green Kit (
Promega
, Cat.#
G8743
) following the manufacturer’s instructions for the endpoint measurement 2X Reagent Addition. Briefly,
(4)
Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors
Vet Res
June 8, 2025
Hana Michova, Jan Pliva, Anezka Jirsova, David Jurnecka, Jana Kamanova
post-
infection
, gentamicin (100 µg/mL) and the fluorescent DNA-binding dye CellTox Green (Cat. No.
G8743
,
Promega
) were added to each well. The plate was subsequently incubated under controlled conditions
(5)
Identification of a TNIK-CDK9 Axis as a Targetable Strategy for Platinum-Resistant Ovarian Cancer
Molecular Cancer Therapeutics
April 2, 2025
Noah Puleo, Harini Ram, Michele L. Dziubinski, Dylan Carvette, Jessica Teitel, Sreeja C. Sekhar, Karan Bedi, Aaron Robida, Michael M. Nakashima, Sadaf Farsinejad,
et al.
culture medium was exchanged for the
staining
solution, containing CellTox Green reagent (
Promega
, #
G8743
, final concentration 1×) and Hoechst 33342 (Sigma, #B2261, final concentration 5 μg/mL). Plates were
(6)
Identification of a TNIK-CDK9 Axis as a Targetable Strategy for Platinum-Resistant Ovarian Cancer
Mol Cancer Ther
January 28, 2025
Noah Puleo, Harini Ram, Michele L. Dziubinski, Dylan Carvette, Jessica Teitel, Sreeja C. Sekhar, Karan Bedi, Aaron Robida, Michael M. Nakashima, Sadaf Farsinejad,
et al.
culture medium was exchanged for the
staining
solution, containing CellTox Green reagent (
Promega
, #
G8743
, final concentration 1×) and Hoechst 33342 (Sigma, #B2261, final concentration 5 μg/mL). Plates were
(7)
Inhibiting ADAM17 enhances the efficacy of olaparib in ovarian cancer spheroids
Scientific Reports
November 6, 2024
Christoph Rogmans, Jan Dittrich, Emily Hamm, Jörg Paul Weimer, David Holthaus, Norbert Arnold, Inken Flörkemeier, Nicolai Maass, Peer Jansen, Astrid Dempfle,
et al.
between those steps. The next day, a final dilution of
1:2000
of the CellTox Green cytotoxicity (
Promega
#
G8743
) dye was added. Three hours later the spheroids were imaged with the same automated imaging systems
(8)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
G3: Genes, Genomes, Genetics
February 7, 2024
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
Coulter, DTX-880).
Cell number
was also estimated using the
CellTox Green Cytotoxicity Assay
(
Promega
,
G8743
) according to manufacturer’s instructions. Single-cell reporter
assays
Single-cell
flow cytometry
-based
(9)
Small-molecule targeting AMPA-mediated excitotoxicity has therapeutic effects in mouse models for multiple sclerosis
Science advances
December 8, 2023
Zhai D, Yan S, Samsom J, Wang L, Su P, Jiang A, Zhang H, Jia Z, Wallach I, Heifets A,
et al
.
prevention of glutamate-mediated excitotoxicity using
CellTox Green cytotoxicity assay
kits (
Promega
,
G8743
) following the manufacturer’s instructions.
HEK293 cells
stably expressing GluA1 and GluA2 at a
1:1
(10)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
bioRxiv
July 27, 2023
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
Coulter, DTX-880).
Cell number
was also estimated using the
CellTox Green Cytotoxicity Assay
(
Promega
,
G8743
) according to manufacturer’s instructions. 4.3.2 Single-cell Reporter
Assays
Single-cell
flow cytometry
-based
(11)
Cholesterol, Amyloid Beta, Fructose, and LPS Influence ROS and ATP Concentrations and the Phagocytic Capacity of HMC3 Human Microglia Cell Line
International Journal of Molecular Sciences
June 20, 2023
Oscar M. Muñoz Herrera, Brian V. Hong, Ulises Ruiz Mendiola, Izumi Maezawa, Lee-Way Jin, Carlito B. Lebrilla, Danielle J. Harvey, Angela M. Zivkovic
cytotoxicity analyses were performed using
Promega
CellTox™ Green Cytotoxicity Assay
(Madison, WI, USA, Cat#:
G8743
), using the End-point method on 96-well plates, at 40,000 cells per 200 µL per well (Supplemental Figures
(12)
A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids
Developmental Cell
June 19, 2023
Wojciech Senkowski, Laura Gall-Mas, Matías Marín Falco, Yilin Li, Kari Lavikka, Mette C. Kriegbaum, Jaana Oikkonen, Daria Bulanova, Elin J. Pietras, Karolin Voßgröne,
et al.
25 μL of the culture medium was exchanged to
staining
solution, containing CellTox Green reagent (#
G8743
,
Promega
, final concentration 1X) and Hoechst 33342 (#B2261, Sigma, final concentration 5 μg/mL). Plates
(13)
BopN is a Gatekeeper of the Bordetella Type III Secretion System
Microbiology Spectrum
June 15, 2023
Kevin Munoz Navarrete, Ladislav Bumba, Tatyana Prudnikova, Ivana Malcova, Tania Romero Allsop, Peter Sebo, Jana Kamanova
as changes in
cell membrane
integrity using the fluorescent DNA-binding dye CellTox Green (Cat. No.
G8743
,
Promega
). In brief, 2 × 104
A549 cells
or 2.5 × 104 Raw 264.7
macrophages
per well were seeded in a
(14)
Targeting and Sensitization of Breast Cancer Cells to Killing with a Novel Interleukin-13 Receptor α2-Specific Hybrid Cytolytic Peptide
Cancers
May 16, 2023
Jannoo R, Walker W, Kanamarlapudi V.
Alamar Blue
assay
. Cell cytotoxicity was assessed using
CellTox™ Green Cytotoxicity Assay
(
Promega
(#
G8743
), Southampton, UK). Briefly, 40,000 cells per well were plated. After 24 h, the medium was replaced
(15)
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma
Science advances
March 29, 2023
Wang D, Yin Z, Wang H, Wang L, Li T, Xiao R, Xie T, Han R, Dong R, Liu H,
et al
.
(500 nM) for 24 hours. Cell death was measured by the
CellTox Green Cytotoxicity Assay
kit (
Promega
, #
G8743
).
Fluorescence
readings were collected with an automatic microplate reader (Molecular Devices). CI values
(16)
A Covalent Calmodulin Inhibitor as a Tool to Study Cellular Mechanisms of K-Ras-Driven Stemness
Frontiers in Cell and Developmental Biology
July 8, 2021
Sunday Okutachi, Ganesh Babu Manoharan, Alexandros Kiriazis, Christina Laurini, Marie Catillon, Frank McCormick, Jari Yli-Kauhaluoma, Daniel Abankwa
The cell viability and cell toxicity effects were analyzed by alamarBlue and CellTox Green (cat. no.
G8743
,
Promega
)
assays
, respectively. A 10% final volume of the alamarBlue reagent was added to each well
(17)
ADAM17 Inhibition Increases the Impact of Cisplatin Treatment in Ovarian Cancer Spheroids
Cancers
April 23, 2021
Hedemann N, Herz A, Schiepanski JH, Dittrich J, Sebens S, Dempfle A, Feuerborn J, Rogmans C, Tribian N, Flörkemeier I,
et al
.
control, the equivalent volume of NaCl was used. The next day, CellToxTM Green cytotoxicity (
Promega
#
G8743
) stain was added in a final dilution of
1:2000
and imaged 3 h later using NYONE® Scientific and the
(18)
Lipid binding by the N-terminal motif mediates plasma membrane localization of Bordetella effector protein BteA
Journal of Biological Chemistry
March 28, 2021
Ivana Malcova, Ladislav Bumba, Filip Uljanic, Darya Kuzmenko, Jana Nedomova, Jana Kamanova
G1780
,
Promega
) or as changes in
cell membrane
integrity using
CellTox Green Cytotoxicity Assay
(cat.no.
G8743
,
Promega
). Experiments were performed according to the manufacturer's instructions. Briefly, 2 x 104
(19)
Direct Phosphorylation and Stabilization of MYC by Aurora B Kinase Promote T-cell Leukemogenesis
Cancer cell
February 10, 2020
Jiang J, Wang J, Yue M, Cai X, Wang T, Wu C, Su H, Wang Y, Han M, Zhang Y,
et al
.
Kits
Annexin V-FITC Apoptosis Kit
,
BioVision
,Cat#
K201
CellTox™ Green Cytotoxicity Assay
kit,
Promega
,Cat#
G8743
Chromatin Immunoprecipitation (ChIP) Assay Kit
,
Sigma-Aldrich
,Cat#
17-295
DAB substrate kit
,
Vector Laboratories
(20)
MEDI3039, a novel highly potent tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptor 2 agonist, causes regression of orthotopic tumors and inhibits outgrowth of metastatic triple-negative breast cancer
Breast cancer research : BCR
February 18, 2019
Greer YE, Gilbert SF, Gril B, Narwal R, Peacock Brooks DL, Tice DA, Steeg PS, Lipkowitz S.
RealTime-Glo™ MT Cell Viability Assay
reagent (cat#
G9712
,
Promega
) and
CellTox™ Green Cytotoxicity Assay
reagent (cat#
G8743
,
Promega
). Live or dead status was monitored by
luminescence
or
fluorescence
(Ex485/Em525) measurement,
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CellTox Green Cytotoxicity Assay from Promega
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