UDP-Glo Glycosyltransferase Assay V6961 from Promega

Product Specs
ItemUDP-Glo Glycosyltransferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6961
Quantity200 assays

Specifications/Features

GenBank / EMBL Accession number : KM359768

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Metabolic control of innate immune activation in TET2-mutant clonal hematopoiesisCell Chem BiolFebruary 19, 2026Peter Geon Kim, Christopher B Hergott, Aidan P Miller, Amy Deik, Meaghan Boileau, Kevin Bullock, Kerry A. Pierce, Angelina H. Choy, Wesley Shin, Marie McConkey, et al.PTMScan® O-GlcNAc [GlcNAc-S/T] Motif Kit,Cell Signaling Technology,Cat#95220 UDP-Glo™ Glycosyltransferase Assay,Promega,Cat#V6961 Glucose Uptake-Glo™ Assay,Promega,Cat#J1342 RNeasy Plus Mini Kit,Qiagen,Cat#74106 NEBNext® Ultra™ RNA Library Prep Kit for Illumina

(2) Inhibition mechanism of the fungal β−1,3-glucan synthases by triterpenoid antifungal drugsNature CommunicationsFebruary 4, 2026Zi-Long You, Lei Sun, Le-Xuan Wang, Yue-Ran Ni, Rui-Qing Lyu, Dan-Dan Chen, Cai-Hong Yun, Tiefeng Song, Yinggai Song, Lin BaiFKS and its mutants was assessed using the UDP-Glo™ Glycosyltransferase Assay (Promega, Catalog No. V6961), which quantifies the activity of glycosyltransferases utilizing UDP-sugars as substrates. Each reaction

(3) A single catalytic domain residue regulates the substrate specificity of a mucin-type O-glycosyltransferase in salivary gland functionbioRxivDecember 29, 2025Pranav Kumar, Navid Tahan Zadeh, Zulfeqhar A. Syed, Hayley M. Reynolds, Duy T. Tran, Liping Zhang, Kelly G. Ten Hagen, Nadine L. Samaraassays Glycosyltransferase activity was measured using the UDP-Glo™ Glycosyltransferase Assay kit (Promega, V6961). A 25 μL reaction was initiated by adding either 10 or 50 nM of purified wild-type and mutant PGANT5A,

(4) WNT signalling promotes NF-κB activation and drug resistance in KRAS-mutant colorectal cancerEMBO ReportsNovember 4, 2025Bojie Cong, Evangelia Stamou, Kathryn Pennel, Teena Thakur, Molly Mckenzie, Amna Matly, Kathryn Gilroy, Harshit Shah, Sindhura Gopinath, Joanne Edwards, et al.iodide,Selleckchem,S6874 CellTiter-Fluor (TM) Cell Viability Assay,Promega,G6082 UDP-Glo™ Glycosyltransferase Assay,Promega,V6961 Software,Software,Software RStudio,Posit,N/A GPT-3.5,Open AI,N/A GraphPad Prism 10,GraphPad Software,N/A Microsoft

(5) The structural basis for the human procollagen lysine hydroxylation and dual-glycosylationNat CommunMarch 11, 2025Junjiang Peng, Wenguo Li, Deqiang Yao, Ying Xia, Qian Wang, Yan Cai, Shaobai Li, Mi Cao, Yafeng Shen, Peixiang Ma, et al.plates, then 5 μL of the UDP detection reagent from UDP-Glo™ Glycosyltransferase Assay kit (Promega, V6961) were added and let incubate at room temperature for 1 h. The plates were then transferred to a Nivo

(6) Protein-adaptive differential scanning fluorimetry using conformationally responsive dyesNature BiotechnologyJanuary 1, 2025Taiasean Wu, Joshua C. Yu, Arundhati Suresh, Zachary J. Gale-Day, Matthew G. Alteen, Amanda S. Woo, Zoe Millbern, Oleta T. Johnson, Emma C. Carroll, Carrie L. Partch, et al.activity. During incubation, the Promega UDP-Glo reagent Promega UDP-Glo Glycosyltransferase assay (Cat # V6961) was prepared. The “UDP-Glo working solution” was prepared by addition of 1 μL “UDP-Glo enzyme” to 74

(7) In Vitro GT-array (i-GT-ray), a Platform for Screening of Glycosyltransferase Activities and Protein–Protein InteractionsBIO-PROTOCOLSeptember 20, 2024Matrika Bhattarai, Tasleem Javaid, Akshayaa Venkataraghavan, Ahmed Faikcatalog number: 31060-T)UDP-GLO Kit (UDP-GloTM Glycosyltransferase Assay) (Promega, catalog number: V6961)GDP-GLO Kit (GDP-GloTM Glycosyltransferase Assay) (Promega, catalog number: VA1090)DOWEX 1X8-100 resin

(8) In Vitro GT-array (i-GT-ray), a Platform for Screening of Glycosyltransferase Activities and Protein–Protein InteractionsBio ProtocSeptember 20, 2024Matrika Bhattarai, Tasleem Javaid, Akshayaa Venkataraghavan, Ahmed Faikcatalog number: 31060-T)UDP-GLO Kit (UDP-GloTM Glycosyltransferase Assay) (Promega, catalog number: V6961)GDP-GLO Kit (GDP-GloTM Glycosyltransferase Assay) (Promega, catalog number: VA1090)DOWEX 1X8-100 resin

(9) Structure of a fungal 1,3-β-glucan synthaseScience AdvancesSeptember 15, 2023Chao-Ran Zhao, Zi-Long You, Dan-Dan Chen, Jing Hang, Zhao-Bin Wang, Meng Ji, Le-Xuan Wang, Peng Zhao, Jie Qiao, Cai-Hong Yun, et al.GT assay The activity of WT Fks1 were measured using the UDP-Glo GT assay from Promega (catalog no. V6961), which can measure the activity of any GT that uses a UDP-sugar as a substrate. Each reaction contained

(10) Structure, catalysis, chitin transport, and selective inhibition of chitin synthaseNat CommunAugust 8, 2023Dan-Dan Chen, Zhao-Bin Wang, Le-Xuan Wang, Peng Zhao, Cai-Hong Yun, Lin BaiUDP-Glo glycosyltransferase assay The activity of Chs1 was measured using the UDP-Glo™ Glycosyltransferase Assay from Promega (Catalog: V6961), which can measure the activity of any glycosyltransferase (GT) that uses a UDP-sugar as a substrate.

(11) Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineeringSTAR ProtocolsMarch 1, 2023Dietlind L. Gerloff, Elena I. Ilina, Camille Cialini, Uxue Mata Salcedo, Michel Mittelbronn, Tanja MüllerPierce BCA protein assay kit,Thermo Fisher Scientific,Cat# 23225 UDP-GloTM glycosyltransferase assay kit,Promega,Cat# V6961 Phusion Hot Start II DNA polymerase,Thermo Fisher Scientific,Cat# F549L QiaQuick gel extraction kit,Qiagen,Cat#28704 Quick

(12) Glycation Interferes with the Activity of the Bi-Functional UDP-N-Acetylglucosamine 2-Epimerase/N-Acetyl-mannosamine Kinase (GNE)BiomoleculesFebruary 23, 2023Hagenhaus V, Gorenflos López JL, Rosenstengel R, Neu C, Hackenberger CPR, Celik A, Weinert K, Nguyen MB, Bork K, Horstkorte R, et al.(Hercules, CA, USA). 2.8. Epimerase Activity Assay (Protein-Ecoli) An UDP Glo™ Glycosyltransferase Assay (V6961; Promega Corporation; Madison, WI, USA) was performed to determine UDP-N-acetyl-glucosamine 2-epimerase

(13) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migrationiScienceFebruary 18, 2022Ilina EI, Cialini C, Gerloff DL, Duarte Garcia-Escudero M, Jeanty C, Thézénas ML, Lesur A, Puard V, Bernardin F, Moter A, et al.assays ,, Duolink Proximity Ligation Assay,Merck,DUO92101 UDP-GloTM Glycosyltransferase Assay,Promega,V6961 ,, Deposited data,Deposited data,Deposited data ,, GLT8D1 interactome dataset,PRIDE repository,PRIDE:

(14) 4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc TransferaseACS Chemical BiologyJanuary 21, 2022Emma G. Jackson et al.In Vitro GALNT Assay GALNT1 and GALNT2 activity was determined in vitro with the UDP-Glo assay (Promega, V6961). The UDP-Glo assay was performed largely as outlined by the manufacturer. Assays were performed in

(15) Chlorovirus PBCV-1 protein A064R has three of the transferase activities necessary to synthesize its capsid protein N-linked glycansProceedings of the National Academy of SciencesNovember 17, 2020Immacolata Speciale et al.prepared in a 96-well plate, performing a serial twofold dilution as described in the UDPGlo kit (code no. V6961, Promega), obtaining 12 solutions at different UDP concentrations. One reaction contained Mn2+ the other

(16) Biochemical and Genetic Analysis Identify CSLD3 as a beta-1,4-Glucan Synthase That Functions during Plant Cell Wall SynthesisThe Plant cellMay 1, 2020Yang J, Bak G, Burgin T, Barnes WJ, Mayes HB, Peña MJ, Urbanowicz BR, Nielsen E.and resuspended with 100;200 mL bead buffer for UDP-Glo or GDP-Glo glycosyltransferase assays (Promega, cat. no. V6961, VA1090). In brief, 500- to 1000-ng proteoliposomes were incubated with 1 mM UDP-Glc in a 20-mL reaction

(17) Angiotensin-(1â€“7) Attenuates Protein O-GlcNAcylation in the Retina by EPAC/Rap1-Dependent Inhibition of O-GlcNAc TransferaseInvestigative Opthalmology & Visual ScienceFebruary 19, 2020Sadie K. Dierschke, Allyson L. Toro, Alistair J. Barber, Amy C. Arnold, Michael D. Dennislysis buffer. OGT activity was assessed using a UDP-Glo glycosyltransferase assay (Promega, catalog #V6961). Bound OGT-HA fusion proteins were resuspended in 50 µL OGT transferase buffer (25 mM HEPES, pH 7.2,

(18) Deploying Fluorescent Nucleoside Analogues for High‐Throughput Inhibitor ScreeningChemBioChemJanuary 15, 2020Leah Seebald, Amaël G. E. Madec, Barbara Imperialithrough. TcdB GT biochemical assays. TcdB GT assays were performed using the UDP-Glo® assay [Promega cat. V6961], which detects UDP release from the glycosyl transferase reactions and provides a quantitative luminescence

(19) Mutations in the Glycosyltransferase Domain of GLT8D1 Are Associated with Familial Amyotrophic Lateral SclerosisCell reportsFebruary 26, 2019Cooper-Knock J, Moll T, Ramesh T, Castelli L, Beer A, Robins H, Fox I, Niedermoser I, Van Damme P, Moisse M, et al.AM1340 LDH Cytotoxicity Assay Kit,Pierce,Cat.# 88953 UDP-glycosyltransferase assay,Promega,Cat.# V6961 UDP-galactose 100mM,Promega,Cat.# V7071 ,, Experimental Models: Cell Lines,Experimental Models: Cell

(20) Structural basis for arginine glycosylation of host substrates by bacterial effector proteinsNature communicationsOctober 16, 2018Park JB, Kim YH, Yoo Y, Kim J, Jun SH, Cho JW, El Qaidi S, Walpole S, Monaco S, García-García AA, et al.as described and GT kinetics were measured using UDP-GloTM Glycosyltransferase Assay kit (Promega, #V6961) by manufacturer’s instruction. Enzyme reaction buffer (ERB) was prepared as 25 mM Tris-HCl [pH 7.5],

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UDP-Glo Glycosyltransferase Assay

UDP-Glo Glycosyltransferase Assay from Promega

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UDP-Glo Glycosyltransferase Assay from Promega

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