NanoBRET Nano-Glo Detection System N1662 from Promega

Product Specs
ItemNanoBRET Nano-Glo Detection System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1662
Quantity1,000 assays

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid BiosensorsAdvanced ScienceFebruary 12, 2026Jeong‐Min Go, Dahee Lee, Minji Kim, Kiseok Han, Gyuho Choi, ChanHui Song, Sanghyun Ahn, Yerim Lee, Jinyoung Lee, Yingxiao Wang, et al.conditions and for the same durations as in the FRET experiments. NanoBRET Nano‐Glo Substrate (Promega, N1662) was added to each well at a final volume of 0.2 µL per well. In all cases, the substrate was added

(2) Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexesCell Mol Life SciFebruary 4, 2026Valentina Elena Wirtgen, Layla Saied, Samuel Zolg, Marta Campos Alonso, Bettina Mayer, Laura Donzelli, Ulrich Maurer, H. T. Marc Timmers, Klaus-Peter Knobeloch, Oded Kleifeld, et al.B,Gibco,"10,687,010" Lipofectamine 3000,Invitrogen™,L3000001 NanoBRET Nano-Glo® Detection Systems,Promega,N1662 Penicillin- Streptomycin,ThermoFisher,15140-122 Phalloidin-iFluor 488,Abcam,ab176753 Phosphate buffered

(3) Functional and structural insights into interactions between β-Arrestin 1 and Gαs or Gαi1Nature CommunicationsJanuary 20, 2026Longhan Duan, Hyunbin Kim, Yeongjun Suh, Donghoon Ahn, Seungmi Kim, Jaekyung Hyun, Yonghoon Kwon, Jihye Seong, Ka Young Chungmedium was replaced with HBSS buffer (pH 7.4) containing 20 mM HEPES, and NanoLuc substrate (Promega, #N1662) was added at a 1:1000 dilution. BRET signals were recorded using a CLARIOstar plate reader (BMG) in

(4) Expanding the toolbox to develop IAP-based degraders of TEAD transcription factorsCommunications chemistryJanuary 19, 2026Gupta N, Trainor N, Radwan M, Nguyen S, Duncan L, Tang AX, Beveridge J, Silke N, Yousef J, Bilgilier C, et al.containing 4% FBS and 0.2 μg/mL doxycycline with or without HaloTag® NanoBRET® 618 Ligand (Promega, N1662) and incubated at 37 °C, 5% CO2 for assay the following day. A 9-points, 1:3 compound dose–response

(5) In vivo measurement of an Apelin gradient with a genetically encoded APLNR conformation biosensorNat CommunJuly 21, 2025Lukas Herdt, Hannes Schihada, Michael Kurz, Sebastian Ernst, Jean Eberlein, Peter Kolb, Cornelius Krasel, Moritz Bünemann, Christian S. M. Helkerwere incubated for 2 min with a 1/1000 solution of the furimazine stock solution (Promega; cat. no. N1662). Three baseline BRET reads were recorded and averaged prior to the addition of 10 µM [Pyr1]-Apelin-13

(6) Identification of novel small molecule chaperone activators for neurodegenerative disease treatmentBiomedicine & PharmacotherapyJune 1, 2025Anita K Ho, Fiona Jeganathan, Magda Bictash, Han-Jou Chenadapted from the technical manual provided in the Promega NanoBRET Protein:Protein Interaction System (N1662, Promega). HEK293T cells were plated in a 6-well plate, or a 10 cm dish the day before transfection.

(7) Regulated induced proximity targeting chimeras-RIPTACs-A heterobifunctional small molecule strategy for cancer selective therapiesCell chemical biologyAugust 15, 2024Raina K, Forbes CD, Stronk R, Rappi JP Jr, Eastman KJ, Zaware N, Yu X, Li H, Bhardwaj A, Gerritz SW, et al.serum,, Critical commercial assays,Critical commercial assays,Critical commercial assays Nano-BRET,Promega,cat# N1662 Cell Titer Glo,Promega,cat# G7572 Halo-Trap,Proteintech,cat# OTA-20 AlphaLISA,PerkinElmer,cat# AS101D;

(8) PDGFRA K385 mutants in myxoid glioneuronal tumors promote receptor dimerization and oncogenic signalingScientific reportsMarch 26, 2024de Villenfagne L, Sablon A, Demoulin JB.Madison, WI, USA) in a 96-well plate. After 4 h, the fluorescent NanoBRET HaloTag 618 Ligand was added (#N1662, Promega) with PDGF-BB. The next day, the medium was removed, the NanoBRET™ NanoLuc Substrate was added,

(9) Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stabilityNature structural & molecular biologyNovember 1, 2023Benabdallah NS, Dalal V, Scott RW, Marcous F, Sotiriou A, Kommoss FKF, Pejkovska A, Gaspar L, Wagner L, Sánchez-Rivera FJ, et al.gel. NanoBRET NanoBRET Protein:Protein Interaction assay was performed following the manufacturer’s conditions (Promega, N1662). Approximately 0.5×106 HEK293T cells were plated the day before transfection in a 12-well plate. Two

(10) RGS5 augments astrocyte activation and facilitates neuroinflammation via TNF signalingJournal of neuroinflammationSeptember 6, 2023Yin S, Ma XY, Sun YF, Yin YQ, Long Y, Zhao CL, Ma JW, Li S, Hu Y, Li MT, et al.(Gibco, 11058021) containing 4% FBS with or without 100 nM HaloTag® NanoBRET™ 618 Ligand (Promega, N1662) per well. Plates were incubated overnight followed by the addition of TNF-α (final concentration, 20

(11) Discovering cell-active BCL6 inhibitors: effectively combining biochemical HTS with multiple biophysical techniques, X-ray crystallography and cell-based assaysSci RepNovember 3, 2022Olivier A. Pierrat, Manjuan Liu, Gavin W. Collie, Kartika Shetty, Matthew J. Rodrigues, Yann-VaÃ¯ Le Bihan, Emma A. Gunnell, P. Craig McAndrew, Mark Stubbs, Martin G. Rowlands, et al.reader. NanoBRET™ For the cellular NanoBRET assay (Promega UK Ltd, NanoBRET Nano-Glo Detection System, catalogue number N1662), DNA encoding full length BCL6 and SMRT was inserted into pFC32K NanoLuc and pFC14K HaloTag vectors

(12) Dual pancreatic adrenergic and dopaminergic signaling as a therapeutic target of bromocriptineiScienceAugust 19, 2022Despoina Aslanoglou, Suzanne Bertera, Laura Friggeri, Marta SÃ¡nchez-Soto, Jeongkyung Lee, Xiangning Xue, Ryan W. Logan, J. Robert Lane, Vijay K. Yechoor, Peter J. McCormick, et al.kit,Cisbio/PerkinElmer,Cat# 62AM4PEB NanoBRET™ Nano-Glo® Detection System,Promega Corporation,Cat# N1662 BCA protein assay kit,Fisher Scientific,Cat# PI23227 RNeasy Plus Micro Kit,Qiagen,Cat# 74034 QuantiTect

(13) Cross-Species Analysis of Innate Immune Antagonism by Cytomegalovirus IE1 ProteinVirusesJuly 26, 2022Franziska Rothemund, Myriam Scherer, Eva-Maria Schilling, Johannes Schweininger, Yves A. Muller, Thomas StammingerInteraction Assay The protein-protein interaction assay was done using the NanoBRET-PPI Kit from Promega (#N1662, Fitchburg, MA, USA). The assay was generally performed following the instructions of the manufacturer.

(14) Development of a BCL-xL and BCL-2 dual degrader with improved anti-leukemic activity,Nature CommunicationsDecember 1, 2021Dongwen Lv et al.plates were incubated at 37 °C, 5% CO2, for 6 h. After treatment, NanoBRET Nano-Glo Substrate (cat. no. N1662, Promega) was added into the medium, and the contents were mixed by shaking the plate for 30 s before

(15) Discovery of a Novel BCL-X(L) PROTAC Degrader with Enhanced BCL-2 InhibitionJournal of medicinal chemistryOctober 14, 2021Pal P, Thummuri D, Lv D, Liu X, Zhang P, Hu W, Poddar SK, Hua N, Khan S, Yuan Y, et al.were incubated at 37 °C, 5% CO2, for 6 h. After treatment, NanoBRET™ Nano-Glo® Substrate (Cat. No. N1662, Promega) were added into the medium and the signals were measured as described previously.10a mBRET

(16) A selective BCL-X(L) PROTAC degrader achieves safe and potent antitumor activityNature medicineDecember 1, 2019Khan S, Zhang X, Lv D, Zhang Q, He Y, Zhang P, Liu X, Thummuri D, Yuan Y, Wiegand JS, et al.were incubated at 37 °C, 5% CO2, for 6 h. After treatment, NanoBRET™ Nano-Glo® Substrate (Cat. No. N1662, Promega) were added into the medium and the contents were mixed by shaking the plate for 30 s before

(17) AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibitionActa pharmacologica SinicaDecember 1, 2019Zhang KL, Shen QQ, Fang YF, Sun YM, Ding J, Chen Y.out the NanoBRET assay. We carried out NanoBRET assays using a NanoBRET Nano-Glo detection System (N1662, Promega) according to the manufacturer’s instructions. Briefly, HEK293T cells were co-transfected with

Be the First to Write a Review!

NanoBRET Nano-Glo Detection System

NanoBRET Nano-Glo Detection System from Promega

Description

Promega

Citations (17)

Reviews

NanoBRET Nano-Glo Detection System from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map