CellTox Green Express Cytotoxicity Assay G8731 from Promega

Product Specs
ItemCellTox Green Express Cytotoxicity Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8731
Quantity200microl
Molecular Weight~61kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Characterization and therapeutic suppression of KEAP1-NRF2-driven resistance to KRAS inhibitors in pancreatic and lung cancerbioRxivApril 24, 2026Wen-Hsuan Chang, Alec J. Vaughan, Addison G. Stamey, Mariana Mancini, Makiko Hayashi, Runying Yang, Ryan Robb, Daniel Andrussier, Jeffrey A. Klomp, Andrew M. Waters, et al.seeded in a 96-well plate with appropriate medium supplemented with CellTox Green Express (Promega #G8731). Cells were incubated overnight, and the baseline fluorescence signals were read using a plate reader

(2) Engineered extracellular vesicles enriched with the miR ‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancerMolecular OncologyFebruary 13, 2026Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, et al.assessed using the MTT assay and the CellTox™ Green Cytotoxicity Assay (Promega, Madison, WI, USA, G8731), respectively [10, 13], as the latter is more sensitive for detecting cytotoxic responses in slowly

(3) SIGLEC12 mediates plasma membrane rupture during necroptotic cell deathNatureJanuary 8, 2026Hyunjin Noh, Zeena Hashem, Elena Boms, Ayaz Najafovpretreatment) + nigericin (20 μM). Ferroptosis was induced with erastin (20 μM). CellToxGreen (Promega, G8731), ToxiLight Non-destructive Cytotoxicity BioAssay (Lonza, LT07-117) and CytoTox 96 Non-Radioactive Cytotoxicity Assay

(4) Monovalent pseudo-natural products supercharge degradation of IDO1 by its native E3 KLHDC3Nature ChemistryJanuary 7, 2026Elisabeth Hennes, Belén Lucas, Natalie S. Scholes, Xiu-Fen Cheng, Daniel C. Scott, Matthias Bischoff, Katharina Reich, Raphael Gasper, María Lucas, Teng Teng Xu, et al.seeding, cells were treated with 10 ng ml−1 (2D) or 1 ng ml−1 (3D) of IFN-γ and CellTox Green (Promega, G8731) and added to the culture medium (1:6,000). The following day, the cells were treated with either DMSO

(5) Proton-boron capture interaction enhances killing of radiation resistant cancer cellsFront OncolDecember 11, 2025Dalong Pang, Mira Jung, Alfredo Velena, William Parke, Anatoly Dritschilodilutions for 48 hours. Cytotoxicity was measured using the CellTox Green Cytotoxicity Assay (Cat.#G8731, Promega), following manufacturer’s instructions. Positive and negative controls were included to ensure

(6) Differential cell survival outcomes in response to diverse amino acid stressLife science allianceNovember 1, 2025Russier M, Fiore A, Bici A, Groß A, Tanzer M, Yeroslaviz A, Mann M, Murray PJ.by analyzing the cell confluence in the phase channel. For cell death measurements, CellTox Green (G8731; Promega) was used according to the manufacturer’s instructions to stain cells with impaired membrane

(7) The class II myosin MYH4 safeguards genome integrity and suppresses tumor progressionJ Clin InvestJune 2, 2025Jayashree Thatte, Ana Moisés da Silva, Judit Börcsök, Thorkell Gudjónsson, Jan Benada, Xin Li, Muthiah Bose, Hanneke van der Gulden, Ji-Ying Song, Renée Menezes, et al.minute and were then incubated at 37°C for 5 days. A solution of 10 μL CellTox Green (1:5000; Promega, G8731) and Hoechst stain (1:1000; Thermo Fisher Scientific, H3570) in PBS was added to the plates using the

(8) The class II myosin MYH4 safeguards genome integrity and suppresses tumor progressionJ Clin InvestJune 2, 2025Jayashree Thatte, Ana Moisés da Silva, Judit Börcsök, Thorkell Gudjónsson, Jan Benada, Xin Li, Muthiah Bose, Hanneke van der Gulden, Ji-Ying Song, Renée Menezes, et al.minute and were then incubated at 37°C for 5 days. A solution of 10 μL CellTox Green (1:5000; Promega, G8731) and Hoechst stain (1:1000; Thermo Fisher Scientific, H3570) in PBS was added to the plates using the

(9) In Vivo and In Vitro Pharmacokinetic Studies of a Dual Topoisomerase I/II InhibitorACS Pharmacology & Translational ScienceApril 11, 2025Jonas Hildebrandt, Dirk O. Bauerschlag, Gert Fricker, Ulrich Girreser, Björn Konukiewitz, Franziska Kellers, Nicolai Maass, Bernd Clement, Inken Flörkemeierdrugs for 48 h. Cell cytotoxicity was assessed using the CellTox Green Cytotoxicity Assay (Promega #G8731) 48 h after treatment with NYONE (SYNENTEC, 485Ex/520Em). Filters: BFEx/GreenEm (530/43 nm); BlueEx

(10) Dual Topoisomerase Inhibitor Is Highly Potent and Improves Antitumor Response to Radiotherapy in Cervical Carcinoma †International Journal of Molecular SciencesMarch 21, 2025Inken Flörkemeier, Hannah L. Hotze, Anna Lena Heyne, Jonas Hildebrandt, Jörg P. Weimer, Nina Hedemann, Christoph Rogmans, David Holthaus, Frank-André Siebert, Markus Hirt, et al.cisplatin or PBS and with or without radiotherapy (section Radiotherapy). CellTox™ Green Assay (Promega G8731, 0.04%, Fitchburg, WI, USA) was added concurrently with treatment. Growth monitoring and cytotoxicity

(11) A phenopushing platform to identify compounds that alleviate acute hypoxic stress by fast-tracking cellular adaptationNat CommunMarch 18, 2025Li Li, Heinz Hammerlindl, Susan Q. Shen, Feng Bao, Sabrina Hammerlindl, Steven J. Altschuler, Lani F. Wuand 1.9 µM MitoTracker™ Deep Red FM (Invitrogen, M22426) in HBSS. CellTox™ Green Cytotoxicity Assay (G8731) was purchased from Promega. The following antibodies were used according to the dilution factors as

(12) Spatial proteomics identifies JAKi as treatment for a lethal skin diseaseNatureNovember 1, 2024Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, et al.14703) or control (DMSO) was added in the indicated concentration as well as Celltox Green (Promega, G8731, 1:8,000 final dilution) to reach a final volume of 200 μl per well. Every 2 h images were acquired

(13) Multicellular ovarian cancer spheroids: novel 3D model to mimic tumour complexityScientific ReportsOctober 9, 2024Inken Flörkemeier, Lisa K. Antons, Jörg P. Weimer, Nina Hedemann, Christoph Rogmans, Sandra Krüger, Regina Scherließ, Astrid Dempfle, Norbert Arnold, Nicolai Maass, et al.PBS for an additional 24 h. Simultaneously with the medium change, the CellTox Green Assay (Promega G8731, Madison, WI) was added in a final dilution of 1:2000 to ensure good dye penetration. Cytotoxicity was

(14) Radiotherapy-induced Immune Response Enhanced by Selective HDAC6 InhibitionMolecular Cancer TherapeuticsDecember 1, 2023Satish Kumar R. Noonepalle, Scott Grindrod, Nima Aghdam, Xintang Li, Maria Gracia-Hernandez, Christian Zevallos-Delgado, Mira Jung, Alejandro Villagra, Anatoly Dritschiloreader (Molecular Devices). Cytotoxicity assays were performed with CellTox Green (Promega, catalog no. G8731) following the manufacturer’s instructions, and fluorescence readings were obtained on SpectraMax i3x

(15) Radiotherapy-induced Immune Response Enhanced by Selective HDAC6 InhibitionMol Cancer TherDecember 1, 2023Satish Kumar R. Noonepalle, Scott Grindrod, Nima Aghdam, Xintang Li, Maria Gracia-Hernandez, Christian Zevallos-Delgado, Mira Jung, Alejandro Villagra, Anatoly Dritschiloreader (Molecular Devices). Cytotoxicity assays were performed with CellTox Green (Promega, catalog no. G8731) following the manufacturer’s instructions, and fluorescence readings were obtained on SpectraMax i3x

(16) A high throughput proliferation and cytotoxicity assay for co-cultured isogenic cell linesMethodsXNovember 15, 2022Syed Ahmad, Kris C. Wood, John E. Scottautomated liquid handling (Biomek NX, Beckman Coulter) Reagents•CellTox Green (Promega, Madison, WI, cat # G8731)•Paclitaxel (Sigma-Aldrich, St. Louis, MO, cat# 33069-62-4)•MycoAlert kit (Lonza, Basel, Switzerland)•trypsin-EDTA

(17) Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian CancerInternational journal of molecular sciencesSeptember 10, 2022Flörkemeier I, Hillmann JS, Weimer JP, Hildebrandt J, Hedemann N, Rogmans C, Dempfle A, Arnold N, Clement B, Bauerschlag DO.72 h and for P8-D6 it was 48 h. Simultaneously to the P8-D6 treatment, CellTox™ Green assay (Promega G8731) was added. Cytotoxicity was detected (485Ex/520Em) and quantified 24 h and 48 h after addition, using

(18) Cellular responses to halofuginone reveal a vulnerability of the GCN2 branch of the integrated stress responseThe EMBO journalJune 1, 2022Pitera AP, Szaruga M, Peak-Chew SY, Wingett SW, Bertolotti A.concentrated HF in culture media to the cells. For HeLa cells, a 1:2,000 dilution of the CellTox Green Dye (#G8731, Promega) was added to the media to assess cytotoxicity by normalizing the Green Dye confluency to Phase

(19) Kynurenine importation by SLC7A11 propagates anti-ferroptotic signalingMolecular cellMarch 3, 2022Fiore A, Zeitler L, Russier M, Groß A, Hiller MK, Parker JL, Stier L, Köcher T, Newstead S, Murray PJ.images per replicate every 2 hr for 48 hr. Cell death was monitored over time using CellTox Green (G8731, Promega) counting for green object count. Cell death analysis by flow cytometry Ferroptosis was induced

(20) Defective transcription elongation in human cancers imposes targetable proteotoxic vulnerabilityTranslational oncologyFebruary 1, 2022Muhammad B, Parks LG, Komurov K, Privette Vinnedge LM.cytotoxicity assay This assay was performed using CellTox Green Cytotoxicity Kit (Promega, Madison, WI, US, G8731) following the manufacturer's instruction. Briefly, human breast cancer cells were seeded in black 96-well

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CellTox Green Express Cytotoxicity Assay

CellTox Green Express Cytotoxicity Assay from Promega

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CellTox Green Express Cytotoxicity Assay from Promega

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