ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Molecular Weight~104kDa
Time20 minutes

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Manganese porphyrin, MnTE-2-PyP, treatment protects the prostate from radiation-induced fibrosis (RIF) by activating the NRF2 signaling pathway and enhancing SOD2 and sirtuin activityFree radical biology & medicineMay 20, 2020Shrishrimal S, Chatterjee A, Kosmacek EA, Davis PJ, McDonald JT, Oberley-Deegan RE.ROS-GLO H2O2 assay Hydrogen peroxide (H2O2) generated by MnTE-2-PyP was measured using the ROS-GLO H2O2 assay (G8820, Promega) as previously described [138]. Briefly, HPrF cells (1 × 104 cells/well in 70 μl of HPrF media)

(122) SAMHD1 Limits the Efficacy of Forodesine in Leukemia by Protecting Cells against the Cytotoxicity of dGTPCell reportsMay 12, 2020Davenne T, Klintman J, Sharma S, Rigby RE, Blest HTW, Cursi C, Bridgeman A, Dadonaite B, De Keersmaecker K, Hillmen P, et al.3/7,Promega,Cat# G6320 TaqMan Universal PCR Master Mix,Thermofisher,Cat# 4304437 ROS-Glo H2O2 assay,Promega,Cat# G8820 ,, Deposited Data,Deposited Data,Deposited Data ,, CyTOF data,This paper,FlowRepository: FR-FCM-Z2JH ,, Experimental Models

(123) Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metforminAgingApril 8, 2020García-Puga M, Saenz-Antoñanzas A, Fernández-Torrón R, Munain AL, Matheu A.1x103 fibroblasts were plated in 96-well plates and grown for 3 days. Afterwards, ROS-Glo H2O2 Assay (G8820, Promega) was performed according to the manufacturer’s instructions. Briefly, a H2O2 substrate reacts

(124) A Commercial Blend of Prebiotic Fiber, Oregano, Thyme, Cinnamon Essential Oils and Yucca Schidigera (One Current™) Supplemented Feed Increased Channel Catfish Fingerling Growth and Enhanced Disease ResistanceJournal of Aquaculture, Marine Biology & EcologyJanuary 1, 2020Beth Peterman, Jessica Koppien-Fox, Lora Petrie-Hansonassay plates with four technical replicates per fish to measure ROS with the ROS-Glo H2O2 assay (Promega, G8820), LDH with the Lactate Dehydrogenase Activity Kit (Sigma, MAK0661KT) and nitrite quantification using

(125) CYP17A1 Maintains the Survival of Glioblastomas by Regulating SAR1-Mediated Endoplasmic Reticulum Health and Redox HomeostasisCancersSeptember 16, 2019Lin HY, Ko CY, Kao TJ, Yang WB, Tsai YT, Chuang JY, Hu SL, Yang PY, Lo WL, Hsu TI.Analysis, and Glutathione Peroxidase (GPx) and Glutathione Reductase (GR) Activity Analysis H2O2-Glo (#G8820) and GSH/GSSG-Glo (#V6611) assay kits were purchased from Promega Inc. and used in accordance with the

(126) Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrityScientific reportsMay 8, 2019Myszor IT, Parveen Z, Ottosson H, Bergman P, Agerberth B, Strömberg R, Gudmundsson GH.h of treatment with HO53 and HO56 (both at 75 µM) was measured using ROS-GloTM H2O2 assay (Promega, G8820) according to the manufacture’s protocol. Agarose gel electrophoresis Semi-quantitative PCR products

(127) G1/S cell cycle regulators mediate effects of circadian dysregulation on tumor growth and provide targets for timed anticancer treatmentPLoS biologyApril 1, 2019Lee Y, Lahens NF, Zhang S, Bedont J, Field JM, Sehgal A.jet-lag cells seeded in a 96-well plate, luminescence-based assays were performed using H2O2 assay kit (G8820, Promega, Madison, WI), NADP/NADPH assay kit (G9081, Promega, Madison, WI), GSH-Glutathione assay kit

(128) Tumor cells induce LAMP2a expression in tumor-associated macrophage for cancer progressionEBioMedicineFebruary 1, 2019Wang R, Liu Y, Liu L, Chen M, Wang X, Yang J, Gong Y, Ding BS, Wei Y, Wei X.treatments as described above, and measured for total ROS production by using ROS-Glo H2O2 Assay (Promega, G8820). All the outcomes were analyzed by luminescence determination in a luminometer. 2.19 Cytotoxicity

(129) Mitochondrial Dysfunction in Aged Macrophages and Lung during Primary Streptococcus pneumoniae Infection is Improved with PirfenidoneScientific reportsJanuary 30, 2019Plataki M, Cho SJ, Harris RM, Huang HR, Yun HS, Schiffer KT, Stout-Delgado HW.cell culture media) hydrogen peroxide generation was assessed using the ROS-Glo H2O2 Assay (Catalog#: G8820, Promega). Mitochondrial respiration was assessed using the MITO-ID Extracellular O2 Sensor Kit (Catalog

(130) Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flowCardio-OncologyDecember 18, 2018Umesh C. Sharma et al.microscope). Measurement of endothelial cell reactive oxidative stress We performed ROS-Glo™ H2O2 assay (Cat No. G8820, Promega) to measure radiation-induced ROS generation in endothelial cells. The assay is based on a

(131) Leflunomide attenuates oxidative stress in fetal human lung endothelial cells via superoxide dismutase 2 and catalaseBiochemical and biophysical research communicationsSeptember 10, 2018Shrestha AK, Menon RT, Shivanna B.generation Hydrogen peroxide (H2O2) levels were quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI; G8820). Briefly, cells grown to 60–70% confluence on 96-well plates were treated with DMSO or leflunomide,

(132) Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1Oxidative Medicine and Cellular LongevityJune 27, 2018Mihaela Temelie, Diana Iulia Savu, Nicoleta MoisoiMeasurement H2O2 was measured using the ROS-Glo Assay Kit (Promega) following the manufacturer's instructions (G8820, Promega). The cells were seeded in 96-well plates. Three hours before the analysis, the medium was

(133) Circulating Exosomes Isolated from Septic Mice Induce Cardiovascular Hyperpermeability Through Promoting Podosome Cluster FormationShockApril 1, 2018Xingjiang Mu et al.assay Author Manuscript The ROS levels in exosomes or MCECs were measured using ROS-Glo H2O2 Assay kit (G8820, Promega) according to the manufacturer's instructions. The luminescence was measured with a Tecan Microplate

(134) Temporal DNA-PK activation drives genomic instability and therapy resistance in glioma stem cellsJCI insightFebruary 8, 2018Wang Y, Xu H, Liu T, Huang M, Butter PP, Li C, Zhang L, Kao GD, Gong Y, Maity A, et al.followed by x-ray irradiation. ROS (H2O2) levels were determined using the ROS-GLO H2O2 assay (Promega, G8820) according to the manufacturer’s instructions. Luminescence was measured using a Synergy H4 Hybrid luminescent

(135) Catalase Enhances Viability of Human Chondrocytes in Culture by Reducing Reactive Oxygen Species and Counteracting Tumor Necrosis Factor-α-Induced ApoptosisCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Li S, Yang X, Feng Z, Wang P, Zhu W, Cui S.System). The cells were mixed with 20 µl of the H2O2 Substrate Solution from the ROS-GloTM H2O2 Assay kit (G8820, Promega) and further incubated for 6 h in the same conditions. Cells in each well were mixed with 100

(136) Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serumJournal of extracellular vesiclesJanuary 1, 2018Kornilov R, Puhka M, Mannerström B, Hiidenmaa H, Peltoniemi H, Siljander P, Seppänen-Kaijansinkko R, Kaur S.After 24 h and 48 h, cells were washed carefully with PBS, and the ROS-GLO H2O2 Assay (Promega Cat. no. G8820/1, lot. no. 0000264704, Fitchburg, WI, USA) was performed according to the manufacturer’s instructions.

(137) Tristetraprolin inhibits mitochondrial function through suppression of α-Synuclein expression in cancer cellsOncotargetJune 27, 2017Vo MT, Choi SH, Lee JH, Hong CH, Kim JS, Lee UH, Chung HM, Lee BJ, Park JW, Cho WJ.luminescence was recorded immediately. Cellular ROS levels were measured using the ROS-Glo™ H2O2 Assay Kit (G8820, Promega) according to the manufacturer's instructions. Briefly, SHSY5Y or HeLa cells were plated on

(138) PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-NitrosylationMolecular cellMarch 16, 2017Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, et al.GSH/GSSG and NOS Activity Measurements H2O2 levels were measured using the ROS-Glo H2O2 assay (Promega, G8820) according to the manufacturer’s instructions. Briefly, 1x104 shGFP control and shPARK2-expressing cells

(139) Omeprazole induces heme oxygenase-1 in fetal human pulmonary microvascular endothelial cells via hydrogen peroxide-independent Nrf2 signaling pathwayToxicology and applied pharmacologyNovember 15, 2016Patel A, Zhang S, Shrestha AK, Maturu P, Moorthy B, Shivanna B.generation Hydrogen peroxide (H2O2) levels was quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI; G8820) according to the manufacturer's recommendation. Briefly, cells were grown on 96-well plates to 60-70%

(140) Src Family Kinases and p38 Mitogen-Activated Protein Kinases Regulate Pluripotent Cell Differentiation in CulturePLOS ONEOctober 10, 2016Boon Siang Nicholas Tan, Joly Kwek, Chong Kum Edwin Wong, Nicholas J. Saner, Charlotte Yap, Fernando Felquer, Michael B. Morris, David K. Gardner, Peter D. Rathjen, Joy Rathjentimes. Biochemical analysis of ROS. ROS production was quantified using the ROS-Glo™ H202 Assay (Promega, G8820). MES cells were seeded at 1 x 106 cells per well into a 96 well white imaging plates (BD Falcon, 351130)

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