m6A RNA Methylation Assay Kit (Colorimetric) ab185912 from Abcam

Product Specs
Itemm6A RNA Methylation Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab185912
Quantity48 Test, 96 Test
Detection Range10 - 500 ng/ml
Assay Time3h 45m
Research AreaEpigenetics
SensitivityEqual to 0.01 ng

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(1) RBM15-Mediated m6A Modification Regulates Proliferation and Migration of Pancreatic Cancer Cells via the lncRNA LINC01320/miR-1287-5p/FBXO11 AxisBiomolecules & TherapeuticsMay 1, 2026Xin Deng, Baosheng Wang(Invitrogen). Tissue and cellular levels of m6A were detected using the m6A RNA methylation quantification kit (ab185912, Abcam, USA). And 200 ng of RNA was added to the wells, followed by the addition of capture and detection

(2) Methyltransferase complex subunit METTL3 maintains genome stability of erythroid cells via MTHFD1-mediated nucleotide biosynthesisJournal of Clinical InvestigationMay 1, 2026Linlin Zhang, Huizhi Zhao, Shihui Wang, Xueting Wu, Donghao Liu, Hengchao Zhang, Qianqian Yang, Ying Cheng, Xiuyun Wu, Jiangwei Zhao, et al.(Vazyme, RC112-01). We analyzed 200 ng total RNA, using the m6A RNA Methylation Quantification Kit (Abcam, ab185912) according to the manufacturer’s instructions. Measurement of blood parameters. Blood parameters of

(3) Mechanism of RBM15 in the Immune Escape of Non-small Cell Lung Cancer Cells Via the LncRNA EGFR-AS1/USP3/PD-L1 AxisBiological Procedures OnlineApril 9, 2026Fang Li, Zhao Zhang, Shuai Liu, Yang Wang, Xiang Deng, Jiasheng Wang3000. The m6A level in the total RNA was determined using the m6A RNA methylation quantification kit (ab185912, Abcam). The m6A levels were quantitatively determined by a colorimetric method based on the absorbance

(4) METTL16-mediated m6A modification of MSMO1 modulates cholesterol metabolism and activates MAPK-p38/NF-κB signaling in colorectal cancerJournal of Experimental & Clinical Cancer ResearchMarch 17, 2026Yongheng Zhao, Tingyue Gong, Hao Li, Haiping Lin, Minhao Yu, Yang Luo, Ming Zhong, Jun QinSangon, China) to verify equal RNA loading. m6A quantitation The m6A RNA Methylation Assay Kit (#ab185912, Abcam, UK) was used to quantify the global m6A levels in total RNA. Total RNA was extracted from paired

(5) USP7 Drives Atherosclerosis by Promoting Ferroptosis in Vascular Endothelial Cells via the KIAA1429/NEAT1/CTCF AxisBiological Procedures OnlineMarch 13, 2026Bo Dong, Lu Kou, Jing-Yu Yang, Yang Li, Ning YangTRIzol, global m6A levels were quantified with the m6A RNA methylation quantification kit (Colorimetric) (ab185912, Abcam) by measuring absorbance at 450 nm with a microplate reader. MeRIP assay was performed using

(6) Methyltransferase complex subunit METTL3 maintains genome stability of erythroid cells via MTHFD1-mediated nucleotide biosynthesisJ Clin InvestMarch 10, 2026Linlin Zhang, Huizhi Zhao, Shihui Wang, Xueting Wu, Donghao Liu, Hengchao Zhang, Qianqian Yang, Ying Cheng, Xiuyun Wu, Jiangwei Zhao, et al.(Vazyme, RC112-01). We analyzed 200 ng total RNA, using the m6A RNA Methylation Quantification Kit (Abcam, ab185912) according to the manufacturer’s instructions. Measurement of blood parameters. Blood parameters of

(7) Mechanism of N6-methyladenosine (m6A)-mediated upregulation of LINC00958 in the growth of cervical cancerFront OncolMarch 6, 2026Yuting Li, Zhengying Liu, Hongling Jinextraction, and RNA quantity was examined using NanoDrop ND-1000. m6A RNA methylation quantification kit (ab185912, Abcam) was employed to test the m6A quality in total RNA, followed by the evaluation of absorbance

(8) Targeting lactylation in a novel metabolic-epigenetic-inflammatory axis mitigates inflammation and promotes healing following corneal injuryJournal of Translational MedicineFebruary 26, 2026Yi Guan, Jinghua Liu, Ying liu, Yarong Yan, Zhengyu Chao, Xinlin Yan, Jiaqi Lin, Yulei Huang, Jiahao Zhu, Shumei Yang, et al.pathways. m6A modification content assessment The m6A RNA Methylation Quantification Kit (Colorimetric) (#ab185912; Abcam, Cambridge, UK) was utilized to measure the m6A content. Briefly, 200 ng of RNA was affixed to

(9) METTL3 Potentiates Corneal Allograft Rejection via Increased Pro-Inflammatory Cytokine Production and Aerobic GlycolysisInvestigative Ophthalmology & Visual ScienceFebruary 24, 2026Huimin Yu, Fanxing Zeng, Junpeng Qu, Xiaoxue Liu, Chaoqun Wei, Chao Wei, Hua GaoMethylation The global level of m6A RNA methylation was quantified using an m6A methylation assay kit (ab185912; Abcam, Waltham, MA, USA) following the manufacturer’s instructions. Briefly, 200 ng of RNA extracted

(10) The effect of Wilms tumor 1-associated protein on ferroptosis and immune escape in non-small-cell lung cancer by mediating N6-methyladenosine modification of Twist1CytojournalFebruary 10, 2026Xianghui Yang, Jiazhi Li, Yueyong Li, Min Li, Luogen Peng, Li Zhao, Lijuan Zoutissues or cells was quantified with N6-methyladenosine RNA (m6A RNA) methylation quantification kits (ab185912, Abcam, Cambridge, the UK). Specifically, the solution was mixed by gently tilting from side to side,

(11) The effect of Wilms tumor 1-associated protein on ferroptosis and immune escape in non-small-cell lung cancer by mediating N6-methyladenosine modification of Twist1CytojournalFebruary 10, 2026Xianghui Yang, Jiazhi Li, Yueyong Li, Min Li, Luogen Peng, Li Zhao, Lijuan Zoutissues or cells was quantified with N6-methyladenosine RNA (m6A RNA) methylation quantification kits (ab185912, Abcam, Cambridge, the UK). Specifically, the solution was mixed by gently tilting from side to side,

(12) Acute myeloid leukemia (AML)-derived bone mesenchymal stem cell exosomal METTL14 promotes AML cell growth and glycolysis by HOXA3/WNT7B axisCell Biology and ToxicologyJanuary 29, 2026Wanying Liu, Xi Ming, Jiaying Wu, Sijia Yan, Shuai Su, Rubing Zheng, Yu Wang, Yi Xiaocells by TRIzol reagent (Invitrogen). Basing on the instructions of m6A RNA methylation Detection Kit (ab185912, Abcam), m6A level (%) was analyzed by a microplate reader. qRT-PCR After extracted total RNA from

(13) METTL14 Mediates Glut3 m6A methylation to improve osteogenesis under oxidative stress conditionRedox ReportDecember 31, 2025Ying Wang, Xueying Yu, Fenyong Sun, Yan Fu, Tingting Hu, Qiqing Shi, Qiuhong ManSigma, St. Louis, MO, USA). The m6A content was assessed utilizing the m6A RNA Methylation Assay Kit (ab185912, Abcam). In this assay, binding solution (80 µl) was mixed with sample RNA(200 ng) and added to designated

(14) Harnessing m6A-regulated cholesterol biosynthesis helps impede tumor burden and stemness in high-garde serous ovarian carcinoma mesenchymal phenotypeMolecular MedicineDecember 29, 2025Aravindan Narayanan, Kiran Dhenge, Sharmila A. Bapatfrom + S/SS A4 and OVMZ6 cells using the m6A RNA Methylation Quantification Colorimetric Kit (Abcam, #ab185912) according to the manufacturer's protocol. For dot blots, 400 ng mRNA (purified with Dynabeads, Invitrogen

(15) METTL3-mediated m6A modification regulates OSCC progression via the HNRNPA2B1/FOXQ1 axisScientific ReportsDecember 9, 2025Xi Wang, Yi Lianalysis RT-qPCR was utilized for cell total RNA extraction. A m6A RNA Methylation Assay Kit (Colorimetric) (ab185912, Abcam) was applied for quantitatively analyzing m6A. After mixing 200 ng RNA with a reagent for RNA

(16) Domain associated with zinc fingers‐containing NF90 ‐ NF45 complex inhibits m 6 A modification of primary microRNA by suppressing METTL3 /14 activityFEBS Open BioDecember 6, 2025Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamotoquantification of pri‐miRNA probes was conducted using a colorimetric m6A RNA methylation assay kit (ab185912; Abcam) in accordance with the manufacturer's protocol. Immunoprecipitation Whole cell extracts from

(17) METTL3-mediated m6A methylation of LncRNA DUXAP8 promoted esophageal squamous cell carcinoma progression by activating the PI3K/AKT signaling pathwayCell DivisionDecember 5, 2025Peiyuan Wang, Suyu Chen, Mengxia Lei, Yujie Chen, Hao He, Peng Chen, Weijie Chen, Hang Zhou, Feng Wang, Derong Zhang(MeRIP) The MeRIP procedure was carried out with the m6A RNA Methylation Assay Kit (Colorimetric) (ab185912, Abcam, UK). Total RNA obtained from TE-1 and KYSE-150 cells was separated and purified. The anti-m6A

(18) IGF2BP3 regulates EMP1 stability in an m6A-dependent manner and activates the TGF-β pathway to promote pancreatic cancer invasionCell Death & DiseaseNovember 24, 2025Long Liu, Yu Zhang, Yuxi Huang, Xiaohong Zhao, Hongxing Fu, Lin Chen, Qi Wang, Xingyu Liu, Xiang Zheng, Hengqing Zhu, et al.detected using a specific capture m6A antibody and detection antibody in an RNA m6A quantification kit (ab185912, Abcam, USA). First, total RNA was added to the incubator along with the capture antibody and cultured

(19) Bushen Zhuluan Decoction alleviates diminished ovarian reserve by regulating the m6A modification of PLGFJournal of Ovarian ResearchNovember 24, 2025Huizhen Liang, Pingfang Tang, Qing Du, Xin Chen, Ling Wangmethylation assay The total level of m6A was examined by a m6A RNA methylation assay kit (colorimetric) (ab185912) purchased from Abcam (Cambridge, UK). The cell lysates were collected for experiments according to

(20) METTL3 inhibits osteoporosis by promoting osteoblast differentiation through m6A-mediated activation of the FZD3/RUNX1 signaling axisEuropean Journal of Medical ResearchNovember 17, 2025Yunfeng Wang, Zhen Wang, Qichuan Zhang, Wenlong Jin, Yunlong Zheng, Xinyue Hureagent. m6A RNA levels in total RNA were detected by colorimetric assay using the m6A Quantification Kit (ab185912, Abcam). RNA immunoprecipitation (RIP) assay According to the Magna RIP kit (Millipore, MA, USA),

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m6A RNA Methylation Assay Kit (Colorimetric) from Abcam

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m6A RNA Methylation Assay Kit (Colorimetric) from Abcam

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