Picro Sirius Red Stain Kit (Connective Tissue Stain) ab150681 from Abcam

Product Specs
ItemPicro Sirius Red Stain Kit (Connective Tissue Stain)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab150681
Sample TypeTissue sections
Assay Time1h 0m
TypeStain
Quantity30 mL, 250 mL

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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Website: http://www.abcam.com

(1) Icariin Attenuates Renal Injury in Streptozotocin-Induced Diabetic Rats with and Without Adenine-Induced Chronic Kidney DiseasePharmaceuticals (Basel)June 22, 2026Raya Al Maskari, Haytham Ali, Priyadarsini Manoj, Mohammed Al Za'abirenal tissue sections were routinely stained with Hematoxylin and Eosin (H & E) and Picrosirius Red (ab150681, Abcam, Cambridge, UK). Renal tubular necrosis was assessed blindly by a pathologist using a semi-quantitative

(2) Hepatocyte TEAD1 drives epithelial-stromal remodeling during cholestatic liver injurybioRxivMay 30, 2026Kumar Kumar, Jeongkyung Jeongkyung, Vinny Vinny, Varun Mandi, Filingeri Filingeri, Danvers Danvers, Rajat Rajat, Samit Ghosh, Mousumi Mousumi, Vijay K Yechoor(H&E) kit (Abcam, ab245880) and liver fibrosis was accessed with Picro Sirius Red staining kit (Abcam, ab150681), as per the manufacturer’s protocol. Immunostaining Paraffin-embedded liver sections were deparaffinized,

(3) A comprehensive approach to elucidating the pathophysiology of kidney fibrosis based on extracellular vesicle proteomicsFrontiers in PhysiologyMay 29, 2026Yaerim Kim, Kyuhyeon Kim, Hong-Beom Park, Sunwha Lee, Mi-yeon Yu, Young Joo Kim, Soo Bin Choi, Woo Yeong Park, Kyubok Jin, Dong Ki Kim, et al.min each. To visualize collagen deposition, the sections were stained with Sirius red (Abcam; Cat# ab150681). After blocking endogenous peroxidase activity with 3% hydrogen peroxide solution (Supelco, Bellefont,

(4) A targetable opioid/cancer associated fibroblast axis drives extracellular matrix remodeling and tumor aggressiveness in pancreatic cancerbioRxivMay 26, 2026Kathryn E. Maraszek, Arwen A. Tisdale, Hunter D. Reavis, Xiaozhuo Liu, Eduardo Cortes Gomez, Aleksandr Dolskii, Caneta Brown, Daksh Thakkar, Maura Dungan, Anusha Adhikari, et al.ethanol: 3 minutes, distilled water: 5 minutes. The Abcam Picro Sirius red stain kit (Abcam, cat. #ab150681) was then used according to the manufacturer’s instructions. In step 3, slides were rinsed in each change

(5) An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selectionCell Reports MedicineMay 19, 2026Fredrik I. Thege, Amber Hoskins, Annaliese Kramer, Intisar Salim, Ashwath Seetharaman, Natalie Fowlkes, Kimal I. Rajapakshe, Anirban Maitra, Sonja M. WoermannB,Illumina,Cat# 20015961 DC Protein Assay,Bio-Rad,Cat# 1705061 Picro Sirius Red staining kit,Abcam,Cat# ab150681 AllPrep DNA/RNA Mini Kit,Qiagen,Cat# 80204 Qubit BR RNA,Thermofisher,Cat# Q10211 Qubit HS DNA,Thermofisher,Cat#

(6) Placental Growth Factor Secreted from Placenta-Derived Mesenchymal Stem Cells Improves Ovarian Function in TAA-Injured Rats via Antioxidant EffectsAntioxidants (Basel)May 10, 2026Jae-Young Shin, Dae-Hyun Lee, Hyeri Park, Young Ran Kim, Gi Jin Kimdeparaffinization using xylene and a graded ethanol series. A Picrosirius Red staining kit (Abcam, ab150681, Cambridge, UK) was subsequently used according to the manufacturer’s protocol. Briefly, the sections

(7) Persistence of alveolar fibroblast-derived ADAMTS4+ cells in a preclinical model of delayed pulmonary fibrosis resolutionNat CommunMay 8, 2026Mahsa Zabihi, Ali Khadim, Arun Lingampally, Ana Ivonne Vazquez-Armendariz, Stefan Hadzic, Georgios-Dimitrios Panagiotidis, Daniel Kalina, Jan Halweg, Tara Procida-Kowalski, Marek Bartkuhn, et al.staining Five-µm-thick paraffin sections were deparaffinized and rehydrated. For Picro Sirius Red staining (Abcam, ab150681), an adequate amount of dye was applied to cover the tissue and incubated for 1 h at room temperature

(8) Protective Effects of Cashew Apple Bagasse and Its Hydroethanolic Extract Against Fatty Liver in RatsAntioxidants (Basel)May 7, 2026Susana Hernández-Pérez, Víctor Hugo Oidor-Chan, Jonathan Puente Rivera, Vicente Castrejón-Téllez, Rosa María Oliart-Ros, Elizabeth Carreón-Torres, Luz Ibarra-Lara, Yanet Chávez-Reyes, Diana Catalina Castro-Rodríguez, Patricia Guillermina Mendoza-García, et al.ranging from 0 to 8 [32]. Adjacent sections were stained according to the picrosirius red stain kit (ab150681; Abcam PLC, Cambridge, UK). Fibrosis was quantified as the collagen deposition proportional area (CPA),

(9) GPR183 contributes to renal macrophage infiltration and fibrosis in kidney injuryAm J Physiol Renal PhysiolMay 1, 2026Qian Zhang, Yan Wang, Yu Fan, Seung Seok Han, Han Xie, Eugene P. Rhee, Donghai Wenparaffin, and sectioned. Sirius Red staining was performed according to the manufacturer’s protocol (ab150681, Abcam) to evaluate collagen deposition and fibrosis. For immunofluorescence, sections were incubated

(10) Type 2 deiodinase–dependent surge in thyroid hormone controls muscle stem cell quiescence and self-renewalJournal of Clinical InvestigationMay 1, 2026Maria Angela De Stefano, Raffaele Ambrosio, Cristina Luongo, Tommaso Porcelli, Daniela Di Girolamo, Caterina Miro, Monica Dentice, Caterina Missero, Domenico Salvatorestaining, sections were fixed with 4% PFA for 10 minutes and stained with Sirius Red solution (Abcam, ab150681) for 60–90 minutes at RT, protected from light. After washing in acidified water, sections were fixed

(11) VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblastsExperimental & Molecular MedicineApril 20, 2026Shan Huang, Qianwen Zhao, Tinghui Shao, Chenghao Zhu, Yujia Xue, Naxia Chen, Yue Zhang, Huihui Xu, Ming Kong, Rui Wang1. Picrosirius Red staining Picrosirius Red (PSR) staining was performed using a commercially available kit (ab150681, Abcam). Following dewaxing and rehydration, paraffin sections were immersed in PSR staining solution

(12) Sympathetic nerve–fibroblast crosstalk drives nerve injury, fibroblast activation, and matrix remodeling in pancreatic cancerJCI InsightApril 8, 2026Ariana L. Sattler, Parham Diba, Kevin Hawthorne, Carl Pelz, Joe Grieco, Tetiana Korzun, Bryan Chong, M.J. Kuykendall, Rosalie C. Sears, Daniel L. Marks, et al.glue. Picro Sirius Red staining Collagen fibers were visualized using the Picro Sirius Red Stain Kit (Abcam, ab150681). FFPE sections were deparaffinized and rehydrated through the following graded series: 3 × 5 minutes

(13) High-Dimensional Protein Analysis Uncovers Distinct Immunological and Stromal Features in Primary and Metastatic Pancreatic Ductal AdenocarcinomaCancer ResearchApril 2, 2026Emily Greene, Natalie K. Horvat, Deon Bryant. Doxie, Vaunita Cohen. Parihar, Jayden Kim, Cameron J. Herting, Erin E. Grundy, Ayana T. Ruffin, Alyssa M. Krasinskas, Shishir K. Maithel, et al.imaging Picrosirius Red staining was performed using the Picrosirius Red Stain Kit for Connective Tissue (Abcam, ab150681) according to the manufacturer’s protocol. Briefly, reagents were equilibrated to room temperature and

(14) ALDH1L2 regulates reactive oxygen species and acinar-to-ductal metaplasia in the pancreasNat MetabApril 1, 2026Marc Hennequart, Loic Mervant, Julie Stockis, Jack Coomes, Martha M. Zarou, Louise Gerard, Virginie Tevel, Valérie Migeot, Steven E. Pilley, Younghwan Lee, et al.and then rinsed with distilled water. The slides were incubated in Picrosirius Red solution (Abcam, ab150681) for 1 h and then drained on absorbent paper. The slides were then rinsed with agitation in 0.5% acetic

(15) Robust immune cell infiltration and macrophage senescence occur within a week of recovery after limb immobilization in older adult skeletal muscleJ PhysiolMarch 27, 2026Chad M. Skiles, Zachary J. Fennel, Paul‐Emile Bourrant, Elena M. Yee, Robert J. Castro, Hannah A. Zabriskie, Melina Itinose, Mark A. Supiano, Ryan M. O'Connell, Micah J. Drummondfibres; satellite cells (Pax7+; catalog. no. AB_528428; DHSB); collagen content (Sirius Red; catalog. no. ab150681; Abcam, Cambridge, UK); immune cell content [haematoxylin (catalog. no. 517‐28‐2; Sigma‐Aldrich, St

(16) Robust immune cell infiltration and macrophage senescence occur within a week of recovery after limb immobilization in older adult skeletal muscleThe Journal of PhysiologyMarch 27, 2026Chad M. Skiles, Zachary J. Fennel, Paul‐Emile Bourrant, Elena M. Yee, Robert J. Castro, Hannah A. Zabriskie, Melina Itinose, Mark A. Supiano, Ryan M. O'Connell, Micah J. Drummondfibres; satellite cells (Pax7+; catalog. no. AB_528428; DHSB); collagen content (Sirius Red; catalog. no. ab150681; Abcam, Cambridge, UK); immune cell content [haematoxylin (catalog. no. 517‐28‐2; Sigma‐Aldrich, St

(17) Nfil3 integrates circadian rhythm and microbial metabolite signaling to maintain gut–liver immune–metabolic homeostasis under high-fat diet stressJournal of Translational MedicineMarch 24, 2026Yung-Ni Lin, Wei-Hao Peng, Yu-Chin Huang, Chi-Yu Lai, Jia-Rou Hsu, Jzy-Yu Wang, Yi-Chu Kao, Li-Ling Wustaining Picrosirius Red Staining was used to observe fibrosis. Picrosirius red (Micro-Sirius Red, Abcam, ab150681) was applied to tissue samples for 60 min. These were rinsed twice with acetic acid and again with pure

(18) The CXCR2–XBP1–NF-κB Axis Drives Inflammatory Amplification and Tissue Damage in Acute Lung InjuryInflammationMarch 23, 2026Zhiyi Liu, Qiong Wu, Xing Liu, Tiantian Ke, Zhisu Yan, Lei Xiong, Fang Tangparaffin-embedded, and sectioned (5 µm). Sections were stained with H&E (Sigma, H3136) or Sirius Red (Abcam, ab150681) for collagen. Immunohistochemistry (IHC) and Immunofluorescence (IF) Sections were incubated with

(19) Modulating Galectin-1 in human osteoblast-like cells alters mineralisation and influences the expression of genes associated with osteoblasts and osteocytesBioscience reportsMarch 18, 2026Ross AC, Feddersen L, Dudhia J, Psifidi A, Guest DJ.objective. For the detection of collagen, fixed cells were stained using a Picrosirius Red stain kit (Abcam, ab150681). Briefly, the fixed cells were hydrated with distilled water and then stained with picrosirius red

(20) Modulating Galectin-1 in human osteoblast-like cells alters mineralisation and influences the expression of genes associated with osteoblasts and osteocytesBioscience ReportsMarch 18, 2026Amy C. Ross, Lauren Feddersen, Jayesh Dudhia, Androniki Psifidi, Deborah J. Guestobjective. For the detection of collagen, fixed cells were stained using a Picrosirius Red stain kit (Abcam, ab150681). Briefly, the fixed cells were hydrated with distilled water and then stained with picrosirius red

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Picro Sirius Red Stain Kit (Connective Tissue Stain) from Abcam

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Picro Sirius Red Stain Kit (Connective Tissue Stain) from Abcam

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