Picro Sirius Red Stain Kit (Connective Tissue Stain) ab150681 from Abcam

Product Specs
ItemPicro Sirius Red Stain Kit (Connective Tissue Stain)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab150681
Quantity30 mL, 250 mL
Sample TypeTissue sections
Assay Time1h 0m
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Type 2 deiodinase–dependent surge in thyroid hormone controls muscle stem cell quiescence and self-renewalJournal of Clinical InvestigationMay 1, 2026Maria Angela De Stefano, Raffaele Ambrosio, Cristina Luongo, Tommaso Porcelli, Daniela Di Girolamo, Caterina Miro, Monica Dentice, Caterina Missero, Domenico Salvatorestaining, sections were fixed with 4% PFA for 10 minutes and stained with Sirius Red solution (Abcam, ab150681) for 60–90 minutes at RT, protected from light. After washing in acidified water, sections were fixed

(2) GPR183 contributes to renal macrophage infiltration and fibrosis in kidney injuryAm J Physiol Renal PhysiolMay 1, 2026Qian Zhang, Yan Wang, Yu Fan, Seung Seok Han, Han Xie, Eugene P. Rhee, Donghai Wenparaffin, and sectioned. Sirius Red staining was performed according to the manufacturer’s protocol (ab150681, Abcam) to evaluate collagen deposition and fibrosis. For immunofluorescence, sections were incubated

(3) Sympathetic nerve–fibroblast crosstalk drives nerve injury, fibroblast activation, and matrix remodeling in pancreatic cancerJCI InsightApril 8, 2026Ariana L. Sattler, Parham Diba, Kevin Hawthorne, Carl Pelz, Joe Grieco, Tetiana Korzun, Bryan Chong, M.J. Kuykendall, Rosalie C. Sears, Daniel L. Marks, et al.glue. Picro Sirius Red staining Collagen fibers were visualized using the Picro Sirius Red Stain Kit (Abcam, ab150681). FFPE sections were deparaffinized and rehydrated through the following graded series: 3 × 5 minutes

(4) High-Dimensional Protein Analysis Uncovers Distinct Immunological and Stromal Features in Primary and Metastatic Pancreatic Ductal AdenocarcinomaCancer ResearchApril 2, 2026Emily Greene, Natalie K. Horvat, Deon Bryant. Doxie, Vaunita Cohen. Parihar, Jayden Kim, Cameron J. Herting, Erin E. Grundy, Ayana T. Ruffin, Alyssa M. Krasinskas, Shishir K. Maithel, et al.imaging Picrosirius Red staining was performed using the Picrosirius Red Stain Kit for Connective Tissue (Abcam, ab150681) according to the manufacturer’s protocol. Briefly, reagents were equilibrated to room temperature and

(5) ALDH1L2 regulates reactive oxygen species and acinar-to-ductal metaplasia in the pancreasNat MetabApril 1, 2026Marc Hennequart, Loic Mervant, Julie Stockis, Jack Coomes, Martha M. Zarou, Louise Gerard, Virginie Tevel, Valérie Migeot, Steven E. Pilley, Younghwan Lee, et al.and then rinsed with distilled water. The slides were incubated in Picrosirius Red solution (Abcam, ab150681) for 1 h and then drained on absorbent paper. The slides were then rinsed with agitation in 0.5% acetic

(6) Robust immune cell infiltration and macrophage senescence occur within a week of recovery after limb immobilization in older adult skeletal muscleThe Journal of PhysiologyMarch 27, 2026Chad M. Skiles, Zachary J. Fennel, Paul‐Emile Bourrant, Elena M. Yee, Robert J. Castro, Hannah A. Zabriskie, Melina Itinose, Mark A. Supiano, Ryan M. O'Connell, Micah J. Drummondfibres; satellite cells (Pax7+; catalog. no. AB_528428; DHSB); collagen content (Sirius Red; catalog. no. ab150681; Abcam, Cambridge, UK); immune cell content [haematoxylin (catalog. no. 517‐28‐2; Sigma‐Aldrich, St

(7) The CXCR2–XBP1–NF-κB Axis Drives Inflammatory Amplification and Tissue Damage in Acute Lung InjuryInflammationMarch 23, 2026Zhiyi Liu, Qiong Wu, Xing Liu, Tiantian Ke, Zhisu Yan, Lei Xiong, Fang Tangparaffin-embedded, and sectioned (5 µm). Sections were stained with H&E (Sigma, H3136) or Sirius Red (Abcam, ab150681) for collagen. Immunohistochemistry (IHC) and Immunofluorescence (IF) Sections were incubated with

(8) Modulating Galectin-1 in human osteoblast-like cells alters mineralisation and influences the expression of genes associated with osteoblasts and osteocytesBioscience ReportsMarch 18, 2026Amy C. Ross, Lauren Feddersen, Jayesh Dudhia, Androniki Psifidi, Deborah J. Guestobjective. For the detection of collagen, fixed cells were stained using a Picrosirius Red stain kit (Abcam, ab150681). Briefly, the fixed cells were hydrated with distilled water and then stained with picrosirius red

(9) Cardiomyocyte-derived TGFB3 attenuates cardiac fibrosis and preserves cardiac function in heart failureScientific ReportsMarch 2, 2026Jizhong Xuan, Jiayu Zhou, Yuanji Huang, Mengyu Huang, Shengyan Pu, Liang Fang, Yuxuan Chen, Jieyuan XueCollagen deposition was assessed by Sirius Red staining using the Picro-Sirius Red Stain Kit (Abcam, ab150681) following the provided protocol. After staining, sections were dehydrated sequentially in absolute

(10) Artificial intelligence‑based quantitative analysis of hepatic fibrosis in carbon tetrachloride-induced mouse model of metabolic dysfunction-associated steatohepatitisToxicological ResearchMarch 1, 2026Jin-Hee Lee, Myung-Hwa Yang, Gyeongjin Han, Won Hoon Jung, Myung Ae Bae, Ji-Seok Han, Tae-Sung Koo, Jae-Woo Cho[27]. Picrosirius Red staining was performed on deparaffinized tissue sections using a commercial kit (ab150681, Abcam, UK) according to the manufacturer’s instructions. Collagen fibers were visualized as red against

(11) Neutrophil extracellular traps drive peritoneal inflammation and tissue remodeling in pediatric peritoneal dialysisPediatric NephrologyMarch 1, 2026Charlotte Maria Dücker, Martin Herrmann, Susanne Boettcher, Sarah Bauer-Carmona, Raphael-Sebastian Schild, Lavinia Schönfeld, Laia Pagerols Raluy, Konrad Reinshagen, Claus Peter Schmitt, Maria Bartosova Medvid, et al.negative control. To differentiate collagen I from III, we stained collagen fibers using Pico Sirius red (ab150681, Abcam) and evaluated them with a polarized light microscopy. Histological sections were automatically

(12) Targeted inhibition of RGS19 alleviates renal fibrosis by restoring autophagy and modulating immune cell infiltrationJournal of NanobiotechnologyFebruary 28, 2026Xinhao Niu, Yufeng Zhao, Long Li, Xiaoqing Xu, Cuidi Xu, Jiaheng Wu, Xiaohan Yu, Yanbo Chen, Ruiming Rong, Bin Xumanufacturer’s protocol, the paraffin-embedded kidney tissue sections were stained with Picrosirius Red (ab150681, Abcam, UK) and Masson’s Trichrome Staining (G1006, Servicebio, Wuhan, China). The positive areas for

(13) Perfusion-Limited Efficacy of Platelet-Rich Plasma in Adipose Tissue GraftsGelsFebruary 22, 2026Hanan Jamal Mohamed, Wonwoo Jeong, Jiwon Choi, Min Kyeong Kim, Jonghyeuk Han, Hyun-Wook KangMasson’s trichrome (Catalog #25088-1, Polysciences, Warrington, PA, USA) and Picrosirius Red (Catalog #ab150681, Abcam, Cambridge, United Kingdom). Masson’s trichrome-stained sections were imaged using a dotSlide

(14) A macrophage-induced subpopulation of mesenchymal cells expressing Fcer1g contributes to wound-induced fibrosisNature CommunicationsFebruary 12, 2026Xinyi Ma, Ergang Wang, Vijitha Puviindran, Ziyuan Su, Xiaoxi Liu, Eijiro Shimada, Chengsong Yan, Yining Liu, Zhenyu Li, Puvi Nadesan, et al.using 1% Acetic Acid for 3 to 5 min. For picrosirius red staining, we followed the Abcam protocol (Cat#ab150681). In brief, slides were mordanted in picrosirius red solution for 1 h and rinsed quickly with 2 changes

(15) Serotonin transporter downregulation is associated with aortic stenosis, and early profibrotic remodeling is mitigated by pharmacological inhibition of HTR2B receptorFrontiers in Cardiovascular MedicineFebruary 12, 2026Dov Levine, Chiara Camillo, Estibaliz Castillero, Emre Bektik, Yaagnik Kosuri, Anthony Campbell, Cary Karcher, Abba Krieger, Liming Pei, Ting Peng, et al.24633-1), and Alizarin Red (Sigma A5533) as markers of calcium deposition. Picrosirius red stain (Abcam AB150681); marker of fibrosis. Standard, single day staining protocols were carried out for Von Kossa, Alizarin

(16) The therapeutic potential of Myoki, a novel peptide in muscle atrophy: mechanisms and applicationsFrontiers in PharmacologyFebruary 12, 2026Eun Mi Kim, Seon Soo Kim, Yong Geon Hyun, Su Yeon Lee, Yong Ji ChungTo assess the degree of muscle fibrosis, they were also stained using a Sirius Red Stain Kit (Abcam, ab150681) according to the manufacturer’s instructions. Both H&E and Sirius Red-stained slides were observed

(17) Acellular Adipose Tissue promotes anti-fibrotic remodeling in Phase II StudymedRxivFebruary 11, 2026Alexis N. Peña, Jordan A. Garcia, Amy E. Anderson, Joel C. Sunshine, Carisa M. Cooney, Pathik Aravind, Joseph Puthumana, Alexander T.F. Bell, Elana J. Fertig, Patrick Byrne, et al.in paraffin, and stained using Hematoxylin & Eosin (H&E) or Picro Sirius red staining (PSR) (Abcam, ab150681) according to standardized procedures. Whole slide H&E samples were imaged with Olympus VS200 Slide

(18) Amyloid β Instigates Cardiac Neurotrophic Signaling Impairment, Driving Alzheimer's Associated Heart DiseaseAdvanced ScienceFebruary 10, 2026Andrea Elia, Rebecca Parodi‐Rullan, Rafael Vazquez‐Torres, Ashley Carey, Huaqing Zhao, Sabzali Javadov, Silvia Fossaticryotome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picrosirius Red Stain Kit (Abcam, ab150681), and images were acquired using a Nikon NiE Fluorescence Microscope with an 20X objective [80]. Cardiac

(19) Fibroblasts of disparate developmental origins harbor anatomically variant scarring potentialCellFebruary 5, 2026Michelle F. Griffin, Dayan J. Li, Kellen Chen, Jennifer B.L. Parker, Jason L. Guo, Seungsoo Kim, Katerina Kraft, Mauricio Downer, Annah G. Morgan, Maxwell M. Kuhnert, et al.(Vector Laboratories, Cat:H-3502), Masson’s Trichrome (Abcam, ab150686), and Picrosirius Red (Abcam, ab150681) staining was performed per standard protocols from the manufacturers. For hematoxylin and eosin-stained

(20) Western Diet-Induced Impairment of Left Atrium Cardiomyocyte Contractility in Female Wistar Rats Is Associated with Slowdown in the Cross-Bridge Cycle and Dephosphorylation of cMyBP-CInt J Mol SciFebruary 3, 2026Elena Mukhlynina, Xenia Butova, Tatiana Myachina, Raisa Simonova, Yulia Antonets, Anna Leiberova, Anastasia Kochurova, Evgeniya Gusarova, Tatiana Chumarnaya, Galina Kopylova, et al.fractional area of atrial collagen was also determined using picrosirius red staining (Picro Sirius Red Kit, ab150681, Abcam, Cambridge, UK). All measurements were performed at 40× objective magnification using a Leica

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Picro Sirius Red Stain Kit (Connective Tissue Stain) from Abcam

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Picro Sirius Red Stain Kit (Connective Tissue Stain) from Abcam

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