Protein Carbonyl Assay Kit (Western Blot) ab178020 from Abcam

Product Specs
ItemProtein Carbonyl Assay Kit (Western Blot)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab178020
Quantity100 Test
Sample TypeAdherent cells, Suspension cells, Tissue Homogenate
Research AreaMetabolism

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(1) Mitochondrial responses to anoxia-reoxygenation exposure in crucian carp ( Carassius carassius )Journal of Experimental BiologyFebruary 15, 2026Gigi Y. Lau, Lucie Gerber, Anette Johansen, Helge-Andre Dahl, May-Kristin Torp, Georgina Bates, Michael P. Murphy, Lars Eide, Kåre-Olav Stensløkken, Göran E. Nilsson, et al.oxidative reactions were detected and quantified using Abcam's Oxidized Protein Western Blot Detection Kit (ab178020). Preparation of extracts were performed following the manufacturer's protocol. Total protein content

(2) Effects of Voluntary Exercise and Acetic Acid Supplementation on Skeletal Muscle Mitochondrial Function in Ovariectomized MiceNutrientsJanuary 20, 2026Ki-Woong Park, Yoonhwan Kim, Yuan Tan, Byung-Jun Ryu, Seung-Min Lee, Hanall Lee, Byunghun So, Jinhan Park, Junho Jang, Chounghun Kang, et al.1 h. Protein carbonylation, a marker of oxidative stress, was measured using a carbonyl assay kit (ab178020, Abcam, Waltham, MA, USA) in combination with 2,4-dinitrophenylhydrazine (DNPH). Detection was performed

(3) Suppressing protein damage response to overcome multidrug resistance in cancer therapyCell discoverySeptember 30, 2025Shao F, Li Z, Xiao H, Chen Y, Zhang Y, Li L, Peng Y, Li X, Hou Y, Li B, et al.lysed, and proteins were extracted and processed using a Protein Carbonyl Content Assay Kit (Abcam, ab178020) according to the manufacturer’s instructions. Briefly, the carbonyl groups in the protein side chains

(4) Vitamin D3 affects liver expression of pro-/anti-inflammatory cytokines and nitric oxide synthases in type 2 diabetesExperimental Biology and MedicineJuly 24, 2025Ihor Shymanskyi, Olha Lisakovska, Mykola Veliky, Olha Mezhenska, Vasyl Bilous, Andrii Siromolot, Anna Khomenko, Dmytro Labudzynskyi, Tetyana Horid’ko, Elvira PasichnaGermany). To measure protein carbonyl groups generated by protein oxidation, a Protein Carbonyl Assay Kit (ab178020, Abcam, UK) for western blot was used according to the manufacturer’s protocol. Quantitation of immunoreactive

(5) Early-Stage Alcoholic Cardiomyopathy Highlighted by Metabolic Remodeling, Oxidative Stress, and Cardiac Myosin Dysfunction in Male RatsInt J Mol SciJuly 15, 2025David V. Rasicci, Jinghua Ge, Adrien P. Chen, Neil B. Wood, Skylar M. L. Bodt, Allyson L. Toro, Alexandra Evans, Omid Golestanian, Md Shahrier Amin, Anne Pruznak, et al.Myosin To assess differences in protein oxidation, a Protein Carbonyl Assay Western Blot Kit (Abcam, ab178020; Cambridge, UK) was utilized. Residual myosin samples from extractions were flash-frozen in liquid nitrogen

(6) Impact of Obesity Caused by a High-Fat Diet on the Heart's Redox BalanceAntioxidants (Basel)June 11, 2025Yildy Utreras-Mendoza, Isidora Mujica Valenzuela, Luis Montecinos, Paulina Donoso, Gina Sánchezderivatized by incubation with 2,4 dinitro phenyl hydrazine (DNPH) using the Protein Carbonyl Assay Kit (ab178020, ABCAM, Waltham, MA, USA). The carbonylated proteins were determined in Western blot, after SDS protein

(7) AA147 Alleviates Symptoms in a Mouse Model of Multiple Sclerosis by Reducing Oligodendrocyte LossGliaJune 1, 2025Metin Aksu, Kevin Kaschke, Joseph R. Podojil, MingYi Chiang, Ian Steckler, Kody Bruce, Andrew C. Cogswell, Gwen Schulz, Jeffery W. Kelly, R. Luke Wiseman, et al.were derivatized by 2,4-dinitrophenylhydrazine (DNPH) using the Protein Carbonyl Assay Kit (Abcam, ab178020). Then, 7μg of derivatized lysate was separated by a 4–12% SDS-PAGE (Bio-Rad, 4561095) and transferred

(8) Hsp90 C-terminal domain inhibition enhances ferroptosis by disrupting GPX4-VDAC1 interaction to increase HMOX1 release from oligomerized VDAC1 channelsRedox BiologyMay 29, 2025Jieyou Li, Guibing Wu, Hairou Su, Manfeng Liang, Shengpei Cen, Yandan Liao, Xiangjun Zhou, Guantai Xie, Zihao Deng, Wenchong Tan, et al.acquired from Cell Signaling Technology (Danvers, MA, USA). Antibodies against GPX4 (ab125066), DNP (ab178020), and VDAC1 (ab154856) were purchased from Abcam (Cambridge, MA, USA). Antibodies against β-Actin (66009-1-Ig),

(9) DNA damage response regulator ATR licenses PINK1-mediated mitophagyNucleic Acids ResMarch 19, 2025Christian Marx, Xiaobing Qing, Yamin Gong, Joanna Kirkpatrick, Kanstantsin Siniuk, Galina V Beznoussenko, Gururaj Rao Kidiyoor, Murat Kirtay, Katrin Buder, Philipp Koch, et al.proteins in cell and brain lysates were labeled with the Oxidized Protein Western Blot Detection Kit (ab178020; Abcam) following the manufacturer’s instruction. mtDNA isolation and mtDNA damage assay The mtDNA

(10) Effects of Vitamin E Intake and Voluntary Wheel Running on Whole-Body and Skeletal Muscle Metabolism in Ovariectomized MiceNutrientsMarch 12, 2025Youngyun Jin, Hee-Jung Yoon, Ki-Woong Park, Hanall Lee, Yuan Tan, Byung-Jun Ryu, Seung-Min Lee, Chae-Eun Cho, Jae-Geun Kim, Nam-Ah Kim, et al.marker, was determined using 2,4-dinitrophenylhydrazine (DNPH) with the protein carbonyl assay kit (ab178020, Abcam Inc., Waltham, MA, USA). Individual protein bands were quantified with a densitometer (Bio-Rad,

(11) Sex Differences in Response to Diet Enriched with Glutathione Precursors in the Aging HeartThe Journals of Gerontology, Series A: Biological Sciences and Medical SciencesJanuary 16, 2025Aude Angelini, Grecia Garcia Marquez, Anna Malovannaya, Marta L Fiorotto, Alexander Saltzman, Antrix Jain, Jo Ann Trial, George E Taffet, Katarzyna A CieslikDRP1 (Proteintech, RRID: AB_2093525; https://www.antibodyregistry.org/AB_2093525), anti-DNPH (kit # ab178020, Abcam), anti-collagen 1α1 (CedarLane #CL50141AP, RRID: AB_10060024; https://www.antibodyregistry.org/AB_10060024),

(12) A glutamine metabolic switch supports erythropoiesisScienceNovember 15, 2024Junhua Lyu, Zhimin Gu, Yuannyu Zhang, Hieu S. Vu, Christophe Lechauve, Feng Cai, Hui Cao, Julia Keith, Valentina Brancaleoni, Francesca Granata, et al.subjected to carbonylation analysis following the instructions from the Protein Carbonyl Assay kit (Abcam ab178020) or by mass spectrometry (LC-MS/MS) detection at the Proteomics Core at UT Southwestern Medical Center. Phenotypic

(13) Deletion of Glyoxalase 1 Exacerbates Acetaminophen-Induced Hepatotoxicity in MiceAntioxidants (Basel, Switzerland)May 25, 2024Dobariya P, Xie W, Rao SP, Xie J, Seelig DM, Vince R, Lee MK, More SS.(Cayman Chemical Co., Cat. No. 10005020, Ann Arbor, MI, USA) and the Dot Blot assay kit (Abcam, Cat. No. ab178020, Waltham, MA, USA). For dot blot analysis, liver homogenates were first derivatized with DNPH as per

(14) Multifaceted roles of t6A biogenesis in efficiency and fidelity of mitochondrial gene expressionNucleic Acids ResearchApril 12, 2024Yong Zhang, Jing-Bo Zhou, Yue Yin, En-Duo Wang, Xiao-Long ZhoutRNA digestion were obtained from Merck and Thermo Fisher Scientific.A protein carbonyl assay kit (ab178020) was purchased from Abcam (Cambridge, UK). Western blot Cells were harvested and subsequently lysed

(15) Autophagy maintains endosperm quality during seed storage to preserve germination ability in ArabidopsisProceedings of the National Academy of SciencesApril 2, 2024Daiki Shinozaki, Erina Takayama, Naoto Kawakami, Kohki Yoshimotowestern blotting with anti-DNP antibody was performed using the oxidized protein western blot detection kit (ab178020; Abcam, Cambridge, United Kingdom). Western blot analysis with anti-GFP, anti-NBR1, and anti-ATG5 antibodies

(16) Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptorMolecular metabolismApril 1, 2024Pinckard KM, Félix-Soriano E, Hamilton S, Terentyeva R, Baer LA, Wright KR, Nassal D, Esteves JV, Abay E, Shettigar VK, et al.performed using a Pierce Co-IP Kit (Thermo Scientific). An Oxidized Protein Western Blot Kit (Abcam; #ab178020) was used to detect oxidation of immunoprecipitated RyR2. The carbonyl groups were derivatized to 2,4-dinitrophenylhydrasone

(17) The Role of Nicotinamide Mononucleotide Supplementation in Psoriasis TreatmentAntioxidants (Basel)February 1, 2024Zhengyi Zhang, Baochen Cheng, Wenqian Du, Mengqi Zeng, Ke He, Tingyi Yin, Sen Shang, Tian Su, Dan Han, Xinyi Gan, et al.from Elabscience (#E-BC-K044, Wuhan, China). A protein carbonyl assay kit was purchased from Abcam (#ab178020, Boston, MA, USA). 2.2. Cell Culture and Treatments HEKa and HaCaT cells were obtained from ATCC (Manassas,

(18) An inorganic mineral-based protocell with prebiotic radiation fitnessNat CommunDecember 5, 2023Shang Dai, Zhenming Xie, Binqiang Wang, Rui Ye, Xinwen Ou, Chen Wang, Ning Yu, Cheng Huang, Jie Zhao, Chunhui Cai, et al.mM, pH 10). Measurement of protein carbonylation was conducted by using Protein Carbonyl Assay Kit (ab178020, Abcam Co. Shanghai, China). Briefly, the carbonyl groups in protein side chains are derivatized to

(19) Modeling aging and retinal degeneration with mitochondrial DNA mutation burdenbioRxivDecember 1, 2023John Sturgis, Rupesh Singh, Quinn Caron, Ivy S. Samuels, Thomas Micheal Shiju, Aditi Mukkara, Paul Freedman, Vera L. Bonilhaquantified in 10 μg of the cell lysates using the Protein Carbonyl Assay Kit (Abcam, Boston, MA, USA, ab178020). Derivatization and neutralization were carried out according to the manufacturer’s protocol. A parallel

(20) Low Glycolysis Is Neuroprotective during Anoxic Spreading Depolarization (SD) and Reoxygenation in LocustseNeuroNovember 6, 2023Yuyang Wang (王宇扬), Alexander G. Little, Maria J. Aristizabal, R. Meldrum Robertsonin ganglionic tissue was measured with Protein Carbonyl Assay kit (Western blotting; Abcam; catalog #ab178020), following manufacturer’s instructions. In short, two MTGs were pooled in each sample and homogenized

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Protein Carbonyl Assay Kit (Western Blot) from Abcam

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Protein Carbonyl Assay Kit (Western Blot) from Abcam

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