Beta Galactosidase Staining Kit (LacZ) ab102534 from Abcam

Product Specs
ItemBeta Galactosidase Staining Kit (LacZ)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab102534
Quantity250 Test
Sample TypeAdherent cells, Suspension cells
TypeStain
ApplicationsEnzyme activity assay
Gene Nameglb1
NCBI Gene AliasesEBP, ELNR1, MPS4B
Research AreaOncology
NCBI Full Gene Namegalactosidase beta 1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Cellular Senescence of Patient-derived Fibroblasts Reveals the Mid-old Stage as a Critical Window for Transcriptomic Signatures Linked to Alzheimer’s Disease Biomarkers and ClassificationClinical Psychopharmacology and NeuroscienceMay 31, 2026Young Joon Cho, Sunwoo Yoon, Yeojin Kim, Ho Min Song, You Jin Nam, Sang Hyuk Lee, Sehee Lee, Donghyuk Shin, Sun Min Lee, So Young Moon, et al.magnification. All procedures followed the manufacturer’s instructions (Senescence β-Galactosidase Staining Kit, ab102534; Abcam). For quantification, fibroblasts from two control and two AD dementia lines were stained at

(2) Bst2-targeted senotherapy restores visual function by eliminating senescent retinal cellsNature CommunicationsMarch 18, 2026Jun Yong Oh, Jae-Byoung Chae, Hyo Kyung Lee, Chul-Woo Park, Minseo Bae, Gyuri Kim, Yujeong Oh, Gyeongseok Yang, Sangpil Kim, Hae Won Ok, et al.staining Senescence was assessed using an SA-β-gal staining kit according to the manufacturer’s protocol (Abcam, ab102534). Briefly, RPE/choroid tissues were fixed for 1 h in a fixative solution, washed with PBS and incubated

(3) Effects of Exosomes From Hypoxia-Induced Adipose-Derived Stem Cells on Ameliorating PhotoagingClin Cosmet Investig DermatolJuly 9, 2025Cuc Bach Huynh, Ngoc Bich Vu, Trung The Van, Phuc Van Phamfiltered PBS for 72 hours. Following this, the cells were fixed using 0.125 mL of Fixative Solution (ab102534, Abcam, UK) at room temperature for 15 minutes, then stained using the Staining Solution Mix from the

(4) Effects of Exosomes From Hypoxia-Induced Adipose-Derived Stem Cells on Ameliorating PhotoagingClin Cosmet Investig DermatolJuly 9, 2025Cuc Bach Huynh, Ngoc Bich Vu, Trung The Van, Phuc Van Phamfiltered PBS for 72 hours. Following this, the cells were fixed using 0.125 mL of Fixative Solution (ab102534, Abcam, UK) at room temperature for 15 minutes, then stained using the Staining Solution Mix from the

(5) Downregulating FGGY carbohydrate kinase domain containing promotes cell senescence by activating the p53/p21 signaling pathway in colorectal cancerInternational Journal of Molecular MedicineMarch 20, 2025Liya Liu, Meizhu Wu, Youqin Chen, Ying Cheng, Sijia Liu, Xinran Zhang, Qiurong Xie, Liujing Cao, Lihui Wei, Yi Fang, et al.temperature. After fixation, the cells were washed and then incubated with β-gal staining solution (cat no. ab102534; Abcam) in a dry incubator at 37°C overnight. Images of the β-gal stained-cells were captured using

(6) Amniotic fluid stem cell conditioned medium’s role in Schwann cell proliferation, survival, and cellular antioxidant activity under normative and oxidative stress conditionsAnnals of Translational MedicineFebruary 28, 2025Chukwuweike Gwam, Nequesha Mohamed, Ayobami S. Ogunsola, Marcel G. Brown, Kaitlin A. Henry, Xue Mafor S-beta-galactosidase using the senescence b-galactosidase staining kit (Abcam, product number: ab102534) (32) according to the manufacturer’s instructions. Positive and negatively stained cells were counted.

(7) Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanomaBMC BiolJanuary 21, 2025Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, et al.enzyme assay The detection of cellular senescence was performed with the Beta Galactosidase Staining Kit (#ab102534, Abcam) according to the manufacturer’s protocol. Bright-field images were acquired by a phase contrast

(8) Metabolites and Metabolic Functional Changes—Potential Markers for Endothelial Cell SenescenceBiomoleculesNovember 20, 2024Jingyuan Ya, Alison Whitby, Ulvi BayraktutanThe activity of S-β-galactosidase (SA-β-gal) was evaluated using a beta-galactosidase staining kit (ab102534, Abcam, Cambridge, UK). In brief, fixed cells were incubated overnight in the staining solution at 37

(9) Investigating the Effects of Chelidonic Acid on Oxidative Stress-Induced Premature Cellular Senescence in Human Skin Fibroblast CellsLife (Basel)August 27, 2024Burcu Turkoglu, Banu Mansurogluthe doubling time analysis. Senescence β-Galactosidase staining was performed using a commercial kit (ab102534 SA-βgal staining Kit, Abcam, Cambridge, MA, USA). From a randomly selected field of view, 7 images were

(10) PAR2‐mediated cellular senescence promotes inflammation and fibrosis in aging and chronic kidney diseaseAging CellAugust 1, 2024Sugyeong Ha, Hyun Woo Kim, Kyung Mok Kim, Byeong Moo Kim, Jeongwon Kim, Minjung Son, Doyeon Kim, Mi‐Jeong Kim, Jian Yoo, Hak Sun Yu, et al.SA‐β‐gal activity using a commercial senescence‐associated β‐galactosidase (SA‐β‐gal) staining kit (ab102534; Abcam, Cambridge, United Kingdom) according to the manufacturer's instructions. Images were obtained

(11) Senolytics and Senomorphics Targeting p38MAPK/NF-κB Pathway Protect Endothelial Cells from Oxidative Stress-Mediated Premature SenescenceCellsJuly 31, 2024Ya J, Bayraktutan U.medium. The SA-β-galactosidase (SA-β-gal) activity was evaluated using a beta-galactosidase staining kit (Ab102534, Abcam, Cambridge, UK) according to the manufacturer’s instructions. In brief, the cells were washed

(12) Loss of the proteasomal deubiquitinase USP14 induces growth defects and a senescence phenotype in colorectal cancer cellsScientific ReportsJune 6, 2024Johannes Gubat, Linda Sjöstrand, Karthik Selvaraju, Kübra Telli, Pádraig D’Arcyfor 48 h. Staining for β-Galactosidase was done using the β-Galactosidase Staining Kit (Abcam, Cat. ab102534) using manufacturer instructions. Photomicrographs were taken at 200 × magnification. β-Galactosidase

(13) Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitroNaunyn-Schmiedeberg's Archives of PharmacologyOctober 18, 2023Keshav Raj Paudel, Dvya Delilaa Clarence, Nisha Panth, Bikash Manandhar, Gabriele De Rubis, Hari Prasad Devkota, Gaurav Gupta, Flavia C. Zacconi, Kylie A. Williams, Lisa G. Pont, et al.purchased from Cell Signalling Technology, Danvers, MA, USA. Beta galactosidase staining (X-gal) kit (ab102534) and goat anti-rabbit Alexa Fluor 647 (ab150079) and goat anti-rabbit Alexa Fluor 488 (ab150077)-conjugated

(14) A Proteomic Approach Identifies Isoform-Specific and Nucleotide-Dependent RAS InteractionsMolecular & cellular proteomics : MCPAugust 1, 2022Miller SP, Maio G, Zhang X, Badillo Soto FS, Zhu J, Ramirez SZ, Lin H.P35G-1.5-14-C) and allowed to incubate overnight. Following the Abcam beta Galactosidase detection kit (ab102534) protocol, cells were washed once with PBS and fixed with 1X fixative solution for 10 min. Cells were

(15) Attenuation of Cigarette-Smoke-Induced Oxidative Stress, Senescence, and Inflammation by Berberine-Loaded Liquid Crystalline Nanoparticles: In Vitro Study in 16HBE and RAW264.7 CellsAntioxidants (Basel)April 28, 2022Keshav Raj Paudel et al.purchased from Cell Signalling Technology, Victoria, Australia. Beta galactosidase staining (X-gal) kit (ab102534) and goat antirabbit Alexa647 (ab150079) antibody were purchased from Abcam, Victoria, Australia. 2.3.

(16) Biofabricating murine and human myo‐substitutes for rapid volumetric muscle loss restorationEMBO Molecular MedicineMarch 5, 2021Marco Costantini et al.A32723, diluted 1:200). LacZ‐positive cells were detected with beta‐galactosidase staining (Abcam, # ab102534), while acetylcholine receptors were detected by α‐bungarotoxin (Alexa Fluor 488®; Invitrogen, # B13422).

(17) Morphological Changes of Porcine Granulosa Cells during in vitro ExpansionPakistan Veterinary JournalApril 1, 2020Hoang Nghia Sonmeasurement was subsequently carried out. β-Galactosidase assay: The beta-Galactosidase Detection Kit (ab102534, Abcam, United States) was employed to assess the expression of β-Galactosidase in pGCs. The pGCs were

(18) Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular FunctionFrontiers in cell and developmental biologyJanuary 1, 2020Eakin AJ, Mc Erlain T, Burke A, Eaton A, Tipping N, Allocca G, Branco CM.fluorescent microscope. Senescence Assay Senescence was assayed using a commercially available beta Galactosidase staining kit (ab102534, Abcam) as per the manufacturer’s instructions. hUVEC were plated onto collagen coated 12-well plates

(19) Biological effects in normal cells exposed to FLASH dose rate protonsRadiotherapy and OncologyOctober 1, 2019Manuela Buonanno, Veljko Grilj, David J. Brennerpercentage of senescent cells one month after irradiation using the β-galactosidase staining kit (Abcam ab102534) following the manufacturer instructions. The samples were observed under a 10× objective at the bright

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Beta Galactosidase Staining Kit (LacZ) from Abcam

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Beta Galactosidase Staining Kit (LacZ) from Abcam

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