Fig 1: LINC01857 inhibition inhibits HCC cell proliferation and promotes apoptosis.HCC cells were infected with sh-LINC01857, with sh-NC as NC. A, LINC01857 expression in HCC cells verified by RT-qPCR. B and C, HCC cell proliferation detected by MTT (B) and colony formation assay (C). D, apoptotic rate assessed by flow cytometry. E, levels of Bax and Bcl-2 in HCC cells measured via ELISA. Three independent repeated tests were conducted. The results were presented as mean ± standard deviation. One-way ANOVA was employed to analyze the data in panels A, C and D. Two-way ANOVA was employed to analyze the data in panels B and E. Tukey’s multiple comparisons test was applied for the post hoc test. ** p<0.01.
Fig 2: ELF3 inhibition reduces proliferation and triggers apoptosis of OC cell lines. A, mRNA expression of ELF3 in cells after sh-ELF3 1, 2 or 3# transfection determined by RT-qPCR (one-way ANOVA, *p < 0.05 compared to sh-NC transfection); B, viability of OC cell lines evaluated by the MTT assay (one-way ANOVA, *p < 0.05); C, proliferation of OC cell lines determined by the colony formation assay (one-way ANOVA, *p < 0.05); D, protein levels of apoptosis-related factors (Bax and Bcl-2) in OC cells measured by ELISA kits; E, apoptosis rate of OC cell lines detected by flow cytometry (one-way ANOVA, *p < 0.05). Data were exhibited as mean ± SD from three independent experiments.
Fig 3: miR-451a promotes cSCC cell apoptosis. (A) Apoptotic A431 and SCC-12 cells transfected with miR-451a mimics or mimic controls were determined by flow cytometric analysis. (B) Hoechst 33258 staining of A431 and SCC-12 cells transfected with the miR-451a mimic or mimic control. Scale bar, 50 µm. (C) Bax and Bcl-2 concentrations in A431 and SCC-12 cells transfected with the miR-451a mimic or mimic control were analyzed using ELISAs. The data are presented as the mean ± SD. One-way ANOVA and Tukey's multiple comparison tests were used to determine statistical significances. *P<0.05. miR, microRNA; cSCC, cutaneous squamous cell carcinoma; PI, propidium iodide.
Fig 4: Analysis of apoptosis genes transcript in HepG-2, MCF-7, Bj-1, and MCF-12F cells treated with MP, MP/IHM, and MP/NabM. Profiling of mRNA transcript levels of key pro- (Casp3 (A), p53 (B), Bax (C) and anti-apoptotic Bcl-2 (D)). Gene expression levels were quantified after 72 h by RT-qPCR employing 18S as a housekeeping gene for normalization as detailed in the methods. Significant differences between the means of individual treatments and control were analyzed by one-side Student’s t-test. Histograms represent mean expression level as fold change SD for 3 technical and 2 biological replicas with different letters (a, b and c) are significantly different (p-value = 0.05). MP: milk proteins concentrate; MP/NabM: milk proteins/Nabeq mucilage complex; MP/IHM: milk proteins/Isabgol husk mucilage complex.
Fig 5: Upregulation of CLDN4 diminishes the suppressing role of ELF3 inhibition in OC progression. A, mRNA expression of CLDN4 after sh-ELF3 or LV-CLDN4 transfection was determined by RT-qPCR (one-way ANOVA,*p < 0.05 compared to sh-NC, #p < 0.05 compared to sh-ELF3 + LV-NC); B-C, viability (B) and proliferation (C) of OC cell lines determined by MTT and cell colony formation assays, respectively (one-way ANOVA, *p < 0.05); D-E, migration (D) and invasion (E) abilities of OC cells measured by scratch test and Transwell assay, respectively (one-way ANOVA, *p < 0.05); F, protein levels of EMT-related biomarkers (E-cadherin and vimentin) detected by western blot analysis (two-way ANOVA, *p < 0.05); G, protein levels of apoptosis-related factors (Bax and Bcl-2) in OC cells measured by ELISA kits; H, apoptosis of OC cells measured by Hoechst 33,258 staining (one-way ANOVA, *p < 0.05). Data were exhibited as mean ± SD from three independent experiments.
Supplier Page from Abcam for Human Bax ELISA Kit