Coenzyme A Assay Kit (Fluorometric - Green) ab138889 from Abcam

Product Specs
ItemCoenzyme A Assay Kit (Fluorometric - Green)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab138889
Sample TypeCell culture extracts, Tissue Extracts, Cell Lysate
SensitivityEqual to 40 nM
Assay Time1h 0m
Quantity200 Test

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Expanding the genetic and clinical spectrum of SLC25A42‐associated disorders and testing of pantothenic acid to improve CoA level in vitroJIMD ReportsNovember 1, 2024Katharina Heckmann, Arcangela Iuso, Janine Reunert, Marianne Grüneberg, Anja Seelhöfer, Stephan Rust, Giuseppe Fiermonte, Eleonora Paradies, Carmela Piazzolla, Manoj Mannil, et al.ab179835). 50 μL of cellular lysate was used for the fluorometric evaluation of total cellular CoA (Abcam, ab138889), and values were normalized to the total protein amount measured using Bradford reagent (Bio‐Rad, #5000001). 4

(2) NINJ1 regulates ferroptosis via xCT antiporter interaction and CoA modulationCell death & diseaseOctober 18, 2024Chen SY, Wu J, Chen Y, Wang YE, Setayeshpour Y, Federico C, Mestre AA, Lin CC, Chi JT.-3’. Intracellular CoA levels Intracellular CoA levels were quantified using the Coenzyme A Assay Kit (Abcam, ab138889) and a fluorescent plate reader, following the manufacturer’s instructions. The results are presented

(3) Bi-Allelic Mutations in Zebrafish pank2 Gene Lead to Testicular Atrophy and Perturbed Behavior without Signs of NeurodegenerationInternational journal of molecular sciencesOctober 26, 2022Mignani L, Zizioli D, Khatri D, Facchinello N, Schiavone M, De Palma G, Finazzi D.Quantification Coenzyme A quantification was performed using a commercial Coenzyme A Assay Kit (Fluorometric–Green, Abcam ab138889). This kit provides a sensitive fluorimetric assay to quantify CoA by detecting the –SH group. Tissue

(4) Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolismCommunications biologyApril 12, 2021Rigby MJ, Lawton AJ, Kaur G, Banduseela VC, Kamm WE, Lakkaraju A, Denu JM, Puglielli L.1.25 for ER fraction). Coenzyme A (CoA) concentrations were determined with a CoA assay kit (Abcam; #ab138889) according to kit instructions using the standard curve. Fluorescence was measured on a GloMax plate

(5) CoAsy knockdown in TNBC cell lines resulted in no overt effect on cell proliferation in vitroBiochemical and Biophysical Research CommunicationsSeptember 1, 2020Hamzah A. Kharabsheh, John E. Scottmaterial. CoA Determination CoA was measured in cell lysates using a fluorescence-based kit (cat# ab138889, Abcam) according to manufacturer’s instructions. Relative fluorescence values were obtained using a

(6) A Homozygous Splice Site Mutation in SLC25A42, Encoding the Mitochondrial Transporter of Coenzyme A, Causes Metabolic Crises and Epileptic EncephalopathyJIMD ReportsJanuary 1, 2018Arcangela Iuso et al.supernatant was recovered and used for the CoA measure according the manufacturer instructions (Abcam, ab138889). The kit is based on an ultrasensitive (detection limit of 40 nM) fluorometric detection of –SH group

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Coenzyme A Assay Kit (Fluorometric - Green) from Abcam

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Coenzyme A Assay Kit (Fluorometric - Green) from Abcam

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