Human IgG ELISA Kit ab195215 from Abcam

Product Specs
ItemHuman IgG ELISA Kit
CompanyAbcam
Price $515.00Supplier Page View Company Product Page
Catalog Numberab195215
Quantity1 x 96 Tests, 1 x 384 Tests
ApplicationssELISA, ELISA
Gene NameIghg1
Detection Range0.23 - 15 ng/ml
SensitivityEqual to 0.02 ng/mL
Research AreaImmunology & Infectious Disease

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Adeno-associated virus delivery of anti-alpha toxin monoclonal antibodies confers protection against Staphylococcus aureus infectionsPLoS PathogApril 6, 2026Josefien W. Hommes, Madison E. Hughes, Derek Cheung, Björn Petri, Lindsey M. Orthner, Tristan J. van der Linden, Bart W. Bardoel, Simran K. Deo, Deepak T. Patel, Ronald S. Flannagan, et al.5000 rpm for 5 minutes to collect plasma. Plasma from blood was run on a Human IgG ELISA kit (Abcam; ab195215) to quantify serum MEDI4893 expression levels at each time point. Six weeks post AAV6.2FF-MEDI4893 injection,

(2) Characterization of Atypical Ebola Virus Disease in FerretsbioRxivJanuary 24, 2026Wenguang Cao, Shihua He, Helene Schulz, Jordan Wight, Michael Chan, Karla Emeterio, Guodong Liu, Jonathan Audet, Kevin Tierney, Kimberly Azaransky, et al.experiments as well as the antibody pharmacokinetics experiment using the Human IgG ELISA Kit (Cat # ab195215, Abcam), according to manufacturer’s instructions. A standard curve was generated in GraphPad Prism

(3) Dinutuximab beta effectively treats Ewing sarcoma when combined with chemotherapyiScienceJanuary 16, 2026Roberta Frapolli, Marina Meroni, Ezia Bello, Lorenza Pirona, Elisa Callegari, Valentina Kebede, Isabella Pellerani, Simone Canesi, Eugenio Scanziani, Uta Dirksen, et al.beta was determined with an ELISA kit for human IgG from AbCam (IgG Human SimpleStep ELISA Kit, Cat. ab195215), according to manufacturer’s instructions. Histology Subcutaneous tumors were harvested and immediately

(4) A B cell-IgA-epithelial axis enhances antitumor immunity and improves outcome in HPV-associated penile squamous cell carcinomaNature CommunicationsDecember 11, 2025Tao Tao, Lianbang Zhu, Deyun Shen, Jiayang Zhang, Panrui Zhang, Zhixi Gu, Dan Cao, Jun Xiao, Wen Panplasma/PSCC tumor tissues were determined using IgA/IgG Human SimpleStep ELISA Kit (Abcam, ab196263 & ab195215). IgA/IgG levels in mouse plasma/subcutaneous tumor tissues were determined using IgA/IgG Mouse SimpleStep

(5) Vaccination protects against COVID-associated pulmonary fibrin depositionJournal of VirologyNovember 25, 2025Joanna Ireland, David Myers, Chang Huang, Cameron Allen, Gwynne Roth, Zhongcheng Zou, Ming Zhao, Motoshi Suzuki, Lisa Olano, Joshua Tan, et al.ELISA ELISA assays were used to determine the levels of fibrinogen (Abcam, ab108841), total IgG (Abcam, ab195215), prothrombin (Innovative Research, IHUFIIKTT), SARS-CoV-2 Spike IgG (Invitrogen, BMS2325), and D-dimer

(6) Integrated antibody language model accelerates IgG screening and design for broad-spectrum antiviral therapyiScienceOctober 17, 2025Hannah F. Almubarak, Wuwei Tan, Andrew D. Hoffmann, Yuanfei Sun, Juncheng Wei, Lamiaa El-Shennawy, Joshua R. Squires, Nurmaa K. Dashzeveg, Brooke Simonton, Yuzhi Jia, et al.Ultra-4 Centrifugal Filter Units 30 kDa MWT,Millipore,Cat# UFC803024 Human IgG ELISA Kit,Abcam,Cat# ab195215 "NucleoSpin 96 RNA, 96-well kit for RNA purification",Macherey-Nagel,Cat# 740709.4 SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX

(7) A novel bispecific integrin α5β1/αv antibody reprograms the Myc-regulated basal phenotype of prostate cancer with natural killer cell–mediated tumor eliminationMolecular Cancer ResearchOctober 2, 2025Raghav Joshi, Ming Zhou, Jeffrey H. Lin, Fei Song, Daniel Fein, Colm Morrissey, Kun Hu, Alexander Poltorak, Paul Mathewfor 20 min at 2000g, and the levels of human IgG in the resulting serum was profiled by ELISA (Abcam #ab195215). Biodistribution studies were performed using a fluorophore (CF750, Biotium #92161) conjugated variant

(8) Increased IGF2 and Immunosuppressive Cell Populations in Ascites of Patients with Recurrent High-Grade Serous Ovarian CancerBiomedicinesAugust 26, 2025Laura F. Mortan, Jacqueline A. Bohn, Doris Mangiaracina Benbrookequal volumes of sample were placed in duplicate wells in a 96-well plate of the IgG ELISA kit. (Abcam #ab195215, Cambridge, UK). The optical density (ODs) endpoints of the assay were corrected by subtracting the

(9) Biomarkers in Aneurysmatic and Spontaneous Subarachnoid Haemorrhage: A Clinical Prospective Multicentre Biomarker Panel Study of S100B, Claudin-5, Interleukin-10, TREM-1, TREM-2 and Neurofilament Light Chain As Well As Immunoglobulin G and MMolecular NeurobiologyApril 28, 2025Thomas Kapapa, Andreas Pfnür, Rebecca Halbgebauer, Patrik Broer, Steffen Halbgebauer, Hayrettin Tumani, Ann-Kathrin Friedrichs, Markus Huber-Lang, Lena Dörfermanufacturer’s instructions. The human IgM ELISA kit (ab137982, Abcam, UK) and the human IgG ELISA kit (ab195215, Abcam, UK) were used to measure the immunoglobulin levels in plasma (serum in control patients) and

(10) Rapid and comprehensive detection of viral antibodies and nucleic acids via an acoustofluidic integrated molecular diagnostics chip: AIMDxSci AdvJanuary 17, 2025Jiao Qian, Jianping Xia, Samantha Chiang, Jessica F. Liu, Ke Li, Feng Li, Fang Wei, Mohammad Aziz, Yong Kim, Vinson Go, et al.(unpurified) versus purified saliva samples was quantified using a standard Human IgG ELISA kit (no. ab195215, Abcam, UK). Before analysis, the samples were diluted 5000× with Sample Diluent NS from the kit. The

(11) Efficacy of cartilage-targeted IGF-1 in a mouse model of growth hormone insensitivityFront Endocrinol (Lausanne)January 9, 2025Krishma Tailor, Janine van Ree, Timothy Stowe, Brit Ventura, Connor Sisk, Joanna Courtis, Anna Camp, Fatima Elzamzami, Jan van Deursen, Robert O'Brien, et al.of CV1574-1 in tissue ( Supplementary Figures 1A, B ) was measured using a hIgG ELISA (Abcam, Cat# ab195215). Adult C57BL/6 WT mice received a subcutaneous injection of 1.5 mg/kg of CV1574-1 (n=3 mice/group).

(12) Evidence of aberrant anti-epstein-barr virus antibody response, though no viral reactivation, in people with post-stroke fatigueJournal of InflammationAugust 12, 2024Isobel C. Mouat, Li Zhu, Alperen Aslan, Barry W. McColl, Stuart M. Allan, Craig J. Smith, Marion S. Buckwalter, Laura McCullochanti-Epstein-Barr nuclear antigen 1 (EBNA1) IgG (Abcam, ab108731), IgM (Abcam, ab214568), IgG (Abcam, ab195215), and CRP (Abcam, ab260058), and completed according to manufacturer’s instructions. Plasma samples

(13) Salivary immunoglobulin levels and periodontal indices in Brazilian children with and without type 1 diabetesBrazilian Oral ResearchMay 13, 2024Thyciana Rodrigues RIBEIRO, Sara Maria SILVA, Renata Asfor Rocha Carvalho MARTINS, Cláudia Ferreira SANTOS, Paulo Goberlânio de Barros SILVA, Adriana Costa e FORTI, Fábio Wildson Gurgel COSTA, Manassés Claudino FONTELES, Cristiane Sá Roriz FONTELESprotocols were used: human protein IgA, Abcam®, Cat. Num. ab137980; human IgG protein, Abcam®, Cat. Num. ab195215; and, human IgM protein, Abcam®, Cat. Num. ab137982 (Sigma – Aldrich Brasil LTDA, São Paulo, Brasil).

(14) Recombinant Newcastle disease viruses expressing immunological checkpoint inhibitors induce a pro-inflammatory state and enhance tumor-specific immune responses in two murine models of cancerFrontiers in MicrobiologyJanuary 24, 2024Lisa A. Santry, Jacob P. van Vloten, Amanda W. K. AuYeung, Robert C. Mould, Jacob G. E. Yates, Thomas M. McAusland, James J. Petrik, Pierre P. Major, Byram W. Bridle, Sarah K. Woottonin serum and tissue homogenate samples was detected using a commercial Human IgG ELISA kit (Abcam, ab195215). To detect sPD-1, a 96-well microplate was coated with 100 μg/well of recombinant mouse PD-L1 (RP-88254,

(15) Peripheral helper-T-cell-derived CXCL13 is a crucial pathogenic factor in idiopathic multicentric Castleman diseaseNature CommunicationsOctober 31, 2023Takuya Harada, Yoshikane Kikushige, Toshihiro Miyamoto, Kazuko Uno, Hiroaki Niiro, Atsushi Kawakami, Tomohiro Koga, Koichi Akashi, Kazuyuki YoshizakiIgE and mouse albumin levels in mouse serum were measured using a commercially available ELISA kit (ab195215, ab196263, ab195216, and ab108792, Abcam) according to the manufacturer’s instructions. In vivo therapeutic

(16) SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin depositionNature communicationsOctober 11, 2023Erickson R, Huang C, Allen C, Ireland J, Roth G, Zou Z, Lu J, Lafont BAP, Garza NL, Brumbaugh B, et al.ELISA ELISA assays were used to determine the levels of fibrinogen (Abcam, ab108841), total IgG (Abcam, ab195215), and prothrombin (Molecular Innovations, HPTKT-TOT) present in human BALF samples. The samples were

(17) Therapeutic blood-brain barrier modulation and stroke treatment by a bioengineered FZD(4)-selective WNT surrogate in miceNature communicationsJune 2, 2023Ding J, Lee SJ, Vlahos L, Yuki K, Rada CC, van Unen V, Vuppalapaty M, Chen H, Sura A, McCormick AK, et al.tubes (Greiner Bio-One). Serum samples were then assessed for L6-F4-2 using a human IgG ELISA Kit (ab195215, Abcam, MA), per manufacturer’s protocol. Pharmacokinetic parameters were estimated using noncompartmental

(18) Mechanisms of Obesity-Induced Changes in Pharmacokinetics of IgG in RatsPharmaceutical ResearchMay 1, 2023Xizhe Gao, Yi-Hua Sheng, Sijia Yu, Jiadong Li, Raymond Rosa, Simone Girgis, Tiffany Guo, Luigi Brunetti, Leonid Kaganhuman IgG and endogenous rat IgG in rat serum was measured using a human IgG SimpleStep® ELISA kit (ab195215) and a rat IgG SimpleStep® ELISA kit (ab189578; Abcam, Cambridge, MA), respectively. The assays were

(19) Relationship between Body Composition and Serum Immunoglobulin Concentrations after Administration of Intravenous Immune Globulin–Preclinical and Clinical EvidencePharmaceuticsFebruary 3, 2023Luigi Brunetti, Helene Chapy, Ronald G. Nahass, Rebecca Moore, Andrew Wassef, Derek Adler, Edward Yurkow, Leonid KaganProcedure Human IgG and rat IgG in the rat serum were quantified using the human IgG SimpleStep® ELISA kit (ab195215; Abcam, Cambridge, MA, USA) and the rat IgG SimpleStep® ELISA kit (ab189578; Abcam, Cambridge, MA, USA),

(20) Protein A-Nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin GAnalytical biochemistryJanuary 1, 2023Nandy S, Crum M, Wasden K, Strych U, Goyal A, Maranholkar V, Mo W, Vu B, Kourentzi K, Willson RC.(KE30001, Proteintech, IL, USA), 3.9 ng/ml (ABIN6952772, Antibodies-online Inc, PA, USA), 200 pg/ml (ab195215, Abcam, MA, USA). 4. Conclusion We demonstrated sensitive IgG detection using recombinant fusion proteins

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Human IgG ELISA Kit from Abcam

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Human IgG ELISA Kit from Abcam

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