Fig 1: Pantothenate and L-carnitine treatment increases histone acetylation levels in KAT6A fibroblasts (P1). Control (C) and mutant P1 fibroblasts (P1) were treated with 0.7 µM pantothenate and 0.7 µM L-carnitine for 15 days (C+ and P1+). (A) Control and KAT6A fibroblasts were incubated with Mitotracker CMXRos FM 100 nM for 45 min, then they were fixed and immunostained with anti-H3K9/K14 and examined by fluorescence microscopy. Fifty cells per condition were analyzed. (B) Histone H3 total acetylation levels in P1 cellular pellets were assessed by the Histone H3 Total Acetylation Colorimetric Detection Fast Kit (Abcam, Hercules, CA, USA, ab115124) protocol. Data represent the mean ± SD of three separate experiments. Absorbance was measured using a POLARstar Omega plate reader (BMG Labtech, Offenburg, Germany). The mitotracker CMX-ROS and H3K9/K14 intensity assessment were performed using FIJI software, as shown in Supplementary Figure S9. * p-value < 0.05 and ** p-value < 0.01. Scale bar = 15 μm. C+ and P1+, treated control and P1 cell lines, respectively.