MitoTox™ Complex II + III OXPHOS Activity Assay Kit ab109905 from Abcam

Product Specs
ItemMitoTox™ Complex II + III OXPHOS Activity Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab109905
Assay Time0h 30m
ApplicationsEnzyme activity assay
Gene NameUQCRC1
NCBI Full Gene Nameubiquinol-cytochrome c reductase core protein 1
NCBI Gene AliasesD3S3191, PKNPY, QCR1, UQCR1
Quantity96 Test

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Website: http://www.abcam.com

(1) TDP-43-driven alternative splicing of UQCRC2 modulates mitochondrial bioenergeticsBiology DirectFebruary 27, 2026Xia Xue, Jingwen Hou, Zhiqi Zhang, Zeting Yang, Le Chang, Zengguang Yang, Simeng Liu, Huang Huang, Lu Mei, Yang Mi, et al.was measured using a colorimetric assay kit (Complex III Enzyme Activity Microplate Assay Kit, Abcam ab109905) according to the manufacturer’s instructions. Briefly, mitochondria were isolated from HEK293T cells

(2) Sodium disrupts mitochondrial energy metabolism to execute NECSONature CommunicationsDecember 13, 2025Yuhui Qiao, Jianghuang Wang, Bohong Wang, Hong Zhou, Qianlin Ni, Wan Fu, Zeping Hu, Qing Zhongmeasurements, using a Microplate Reader (Perkin Elmer). The MitoTox Complex II + III OXPHOS Activity Assay Kit (ab109905, Abcam) was used to evaluate Complex II/III activity. Activity solution was combined with a range of

(3) Mechanistic study of pexidartinib-induced toxicity in human hepatic cellsChemico-Biological InteractionsOctober 8, 2025Si Chen, Yuxi Li, Xilin Li, Nan Mei, Xiaobo He, Matthew S. Bryant, Xuan Qin, Feng Li, Lei Guoof mitochondrial complex III activity: A MitoTox™ Complex III OXPHOS Activity Assay kit (Catalog # ab109905, Abcam) was used following the manufacturer’s protocol to assess the direct inhibitory effect of pexidartinib

(4) Mesencephalic Astrocyte‐Derived Neurotrophic Factor Binds BAX to Preserve Mitochondrial Homeostasis and Energy Metabolism for Relieving Myocardial HypertrophyAdvanced ScienceJuly 30, 2025Dong Wang, Xinru Zhang, Baolong Wang, Haipeng Li, Dongshuo Xu, Yun Yang, Jialu Zhang, Wenbing Wang, Ren Zhang, Xinyu Wang, et al.Enzyme Activity Assay Kit (Abcam, ab109721), MitoTox Complex II+III OXPHOS Activity Assay Kit (Abcam, ab109905), and Complex IV Rodent Enzyme Activity Microplate Assay Kit (Abcam, ab109911) were used for activity

(5) OXPHOS inhibition overcomes chemoresistance in triple negative breast cancerRedox BiolApril 15, 2025Cemile Uslu, Eda Kapan, Alex Lyakhovichmitochondrial OXPHOS Complex I (NADH dehydrogenase) and Complex III were measured with kits (ab109721 and ab109905, respectively, both from Abcam, UK) according to the manufacturer's manual. The specific enzymes were

(6) Elevated meteorin-like protein from high-intensity interval training improves heart function via AMPK/HDAC4 pathwayGenes & DiseasesNovember 1, 2024Yongshun Wang, Jie Yuan, Huadong Liu, Jie Chen, Jieru Zou, Xiaoyi Zeng, Lei Du, Xin Sun, Zhengyuan Xia, Qingshan Geng, et al.commercially available enzyme assays, in accordance with the manufacturer's instructions (Cat # ab109721, ab109905, ab109911, ab109714; Abcam). Cardiac tissues were manually homogenized in mitochondrial isolation buffer

(7) Toxoplasma gondii macrophage migration inhibitory factor shows anti-Mycobacterium tuberculosis potential via AZIN1/STAT1 interactionScience advancesOctober 25, 2024Yoon C, Kim HK, Ham YS, Gil WJ, Mun SJ, Cho E, Yuk JM, Yang CS.Complex I, II-III, IV, and V OXPHOS Activity Microplate Assay kits from Abcam (ab109903, ab109904, ab109905, ab109907, respectively, Cambridge, MA), following the manufacturer’s instructions. The activity of

(8) NARFL deficiency caused mitochondrial dysfunction in lung cancer cells by HIF-1α–DNMT1 axisScientific ReportsOctober 11, 2023Hongzhou Liu, Xueqin Wu, Tianrong Yang, Chen Wang, Fei Huang, Ying Xu, Jie Pengmeasurement Complex activities were measured using the complex assay kit (Abcam, ab109721 for Complex I activity, ab109905 for Complex II + III, ab109911 for Complex IV and ab109714 for Complex V) in mitochondrial fractions.

(9) Low-Dose Radiation Reduces Doxorubicin-Induced Myocardial Injury Through Mitochondrial PathwaysDose-response : a publication of International Hormesis SocietyJanuary 1, 2023Zhao D, Jiang X, Meng X, Liu D, Du Y, Zhao L, Jiang H.I–V were measured using enzyme assay kits according to the manufacturer’s protocols (Abcam: ab109721, ab109905, ab109911, and ab109714). Mitochondrial pellets were suspended in PBS supplemented with 10% detergent

(10) Identification of Dihydroorotate Dehydrogenase Inhibitors Using the Cell Painting AssayChemBioChemNovember 18, 2022Beate Schölermann, Jana Bonowski, Michael Grigalunas, Annina Burhop, Yusheng Xie, Joseph G. F. Hoock, Jie Liu, Mark Dow, Adam Nelson, Christine Nowak, et al.(compound 1) were provided by the Structural Genomics Consortium. Complex II+III OXPHOS Activity Assay Kit (ab109905) was purchased from Abcam. Cell lines: HCT116 cells (Cat# ACC 581 DSMZ, Germany) and U2OS cells were

(11) Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulationExperimental and Therapeutic MedicineJanuary 24, 2022Min Lei, Guibao Ke, Yan Wang, Dan Luo, Yao Huin microplates using specific kits: Complex I (cat. no. ab109721; Abcam) and complex III (cat. no. ab109905; Abcam) according to the manufacturer's instructions. The activity of the mitochondrial complexes was

(12) NKX3.1 Localization to Mitochondria Suppresses Prostate Cancer InitiationCancer DiscoverySeptember 1, 2021Alexandros Papachristodoulou et al.Abcam, Cambridge, MA, USA), complex II (700940, Cayman Chemical, Ann Arbor, MI, USA), complex II+III (ab109905, Abcam, Cambridge, MA, USA), complex IV (ab109906, Abcam, Cambridge, MA, USA), and complex V (ab109907,

(13) The BRD7-P53-SLC25A28 axis regulates ferroptosis in hepatic stellate cellsRedox biologySeptember 1, 2020Zhang Z, Guo M, Shen M, Kong D, Zhang F, Shao J, Tan S, Wang S, Chen A, Cao P, et al.complex III activity was determined using MitoTox™ Complex II + III OXPHOS Activity Assay Kit (Abcam, ab109905). Isolated mitochondria were incubated with oxidized cytochrome c on pre-coated 96-well microplate,

(14) Withania somnifera Extract Enhances Energy Expenditure via Improving Mitochondrial Function in Adipose Tissue and Skeletal MuscleNutrientsFebruary 7, 2020Lee DH, Ahn J, Jang YJ, Seo HD, Ha TY, Kim MJ, Huh YH, Jung CH.of complex III and complex IV from isolated mitochondria was measured using a microplate assay kit (ab109905 and ab109911 respectively; Abcam) according to manufacturer’s instruction. 2.13. LC-MS/MS Analysis The

(15) Deletion of heat shock protein 60 in adult mouse cardiomyocytes perturbs mitochondrial protein homeostasis and causes heart failureCell Death & DifferentiationFebruary 1, 2020Feifei Fan et al.microplate assay kits (complex I, catalog no. ab109721; complex II, catalog no. ab109908; complex III, catalog no. ab109905; complex IV, catalog no. ab109911; Abcam). The signal was quantified using a spectrophotometer according

(16) Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPKCell reportsNovember 5, 2019Wang Y, An H, Liu T, Qin C, Sesaki H, Guo S, Radovick S, Hussain M, Maheshwari A, Wondisford FE, et al.MitoTox Complex I OXPHOS Activity Assay Kit (ab109903),Abcam,Cat# ab109903 MitoTox Complex II + III OXPHOS Activity Assay Kit (ab109905),Abcam,Cat# ab109905 MitoTox Complex IV OXPHOS Activity Assay Kit (ab109906),Abcam,Cat# ab109906 MitoTox Complex V OXPHOS Activity Assay Kit

(17) Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia‑reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunctionExperimental and Therapeutic MedicineJanuary 29, 2019Jie Hao, Hong Du, Fan Liu, Jing‑Chao Lu, Xiu‑Chun Yang, Wei Cuidehydrogenase (complex II; cat. no. ab109908), coenzyme Q-cytochrome c oxidoreductase (complex III; cat. no. ab109905) and cytochrome c oxidase (complex IV; cat. no. ab109911), were analyzed using commercial kits (Abcam)

(18) Nanocurcumin–pyrroloquinoline formulation prevents hypertrophy–induced pathological damage by relieving mitochondrial stress in cardiomyocytes under hypoxic conditionsExperimental & Molecular MedicineDecember 1, 2017Sarita Nehra, Varun Bhardwaj, Anju Bansal, Pronobesh Chattopadhyay, Deepika SaraswatWaltham, MA, USA) and subjected to analysis of Complex I (AAMT001, Millipore), Complex II and III (ab109905, Abcam), Complex IV (ab109911, Abcam) and Complex V (AAMT005, Millipore) activities using commercially

(19) Targeting cancer cells through antibiotics-induced mitochondrial dysfunction requires autophagy inhibitionCancer LettersSeptember 20, 2016Milan Esner, Dmitry Graifer, Matilde E. Lleonart, Alex Lyakhovich

(20) The Mitochondrial Unfoldase-Peptidase Complex ClpXP Controls Bioenergetics Stress and MetastasisPLOS BiologyJuly 7, 2016Jae Ho Seo et al.phosphorylation complex activity using Abcam reagents (Cat. no. ab109721—Complex I, ab109908—Complex II, ab109905—Complex II/III, ab109909—Complex IV) and Cayman reagent (701000—Complex V) using isolated lysed mitochondria,

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MitoTox™ Complex II + III OXPHOS Activity Assay Kit from Abcam

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MitoTox™ Complex II + III OXPHOS Activity Assay Kit from Abcam

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