Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation ab65359 from Abcam

Product Specs
ItemCholesterol/ Cholesteryl Ester Assay Kit - Quantitation
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65359
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay Time1h 0m
Quantity100 Test, 2000 Test

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(1) SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2Communications BiologyFebruary 7, 2026Yumeng Zhang, Yuheng Shao, Xin Li, Dongdong Zhou, Jinglan Zhou, Qin Yan, Wei Gao, Liang Yangcholesterol esters) following the manufacturer’s protocol for the Cholesterol/Cholesteryl Ester Assay kit (ab65359, Abcam, Cambridge, MA, USA). Free cholesterol was detected without adding cholesterol esterase. After

(2) Increased atherosclerosis and expression of inflammarafts in macrophage foam cells in AIBP-deficient mice.Scientific ReportsFebruary 7, 2026Shenglin Li, Nicolaus Nazarenkov, Elena Alekseeva, Soo-Ho Choi, Juliana Maria Navia-Pelaez, Aakash Patel, Patrick Secrest, Philip L.S.M. Gordts, Sven Heinz, Yury I. Millertriglyceride levels in plasma samples were quantified using a cholesterol/cholesteryl ester assay kit (ab65359, Abcam) and a triglyceride assay kit (ab65336, Abcam), respectively, according to the manufacturer’s

(3) Acetylation of fatty acid synthase regulates microglial lipid droplets accumulation and pro-inflammatory activity following traumatic brain injuryRedox BiologyFebruary 1, 2026Fengchen Zhang, Tao Lv, Tianqi Xu, Jie Li, Jie Lian, Siyu Lin, Qin Hu, Yichao Jin, Feng Jia, Xiaohua Zhang(Triglyceride Assay Kit – Quantification, Abcam, ab65336; Cholesterol/Cholesteryl Ester Assay Kit – Quantitation, Abcam, ab65359; Free Fatty Acids Content Assay Kit, Solarbio, BC0595) according to the manufacturer's protocols. 4.10

(4) Human Myelin Spheres for in Vitro Oligodendrocyte Maturation, Myelination and Neurological Disease ModelingStem Cell Reviews and ReportsJanuary 28, 2026Karan Ahuja, Roya Ramezankhani, Xinyu Wang, Thibaut Burg, Giulia Amos, Katrien Neyrinck, Alessio Silva, Geethika Arekatla, Eleanor Eva Cassidy, Fatemeharefeh Nami, et al.pellet was used for cholesterol measurement using the Cholesterol/Cholesteryl Ester Assay Kit (Abcam, #ab65359) according to the manufacturer’s protocol. A total of 120 ng of DNA from each sample was used to measure

(5) Effects of Ritonavir, Lopinavir, and Alcohol on ABC Transporters and Secretion of Bile Acid and Bilirubin in Senescent HepatocytesInternational Journal of Molecular SciencesJanuary 25, 2026Liting Chen, Eric Duran, Diego Headrick, Cheng Jitemperature. Total intracellular cholesterol was measured using the Cholesterol Assay Kit from Abcam (Cat No. AB65359). To evaluate cell death, PHHs were stained with Sytox green (1 mM; Molecular Probes, Eugene, OR, USA)

(6) Mitochondrial HMGCS1 mediates cisplatin resistance in cervical cancer through regulation of mitochondrial transcriptionBMC Molecular and Cell BiologyJanuary 16, 2026Wenxuan Yang, Shumin Liu, Dandan Shang, Ping Liu, Hongtang Shi, Hongtao Zhang, Chao Zhoucholesterol levels were measured using the Cholesterol/Cholesteryl Ester Quantitation Assay Kit (Abcam, ab65359) according to the manufacturer’s instructions. Briefly, cells (1 × 106) were washed with cold PBS and

(7) The transcription factor HHEX maintains glucocorticoid levels and protects adrenals from androgen-induced lipid depletionNature CommunicationsJanuary 11, 2026Typhanie Dumontet, Kaitlin J. Basham, Micah C. Foster, Emma Silverman, Kyle A. Heard, Dominque Johnson, Chaelin Lee, Samuel W. Plaska, David T. Breault, David Penton, et al.of lipid extraction solution (Chloroform, Isopropanol, Igepal) as described by manufacturer (Abcam, #ab65359) with slight modifications. Adrenal tissues were homogenized using a BeadBug™ Microtube Homogenizer

(8) Cardiac fibrosis induced by high‐fat diet in ApoE ‐deficient male mice is exacerbated by genetic deletion of PACAP – PAC1 signalingJournal of NeuroendocrinologyJanuary 1, 2026J. M. Schubart, M. K. H. Schaefer, G. A. Bonaterra, L. Mey, H. Schwarzbach, S. Pankuweit, F. Ausbuettel, L. E. Eiden, S. Weyand, E. Weihe, et al.Switzerland) together with commercially available assay kits (Cholesterol/Cholesteryl Ester Quantitation Kit ab65359 and Triglyceride Quantification Assay Kit ab65336; Abcam, Cambridge, UK), according to the manufacturers'

(9) Harnessing m6A-regulated cholesterol biosynthesis helps impede tumor burden and stemness in high-garde serous ovarian carcinoma mesenchymal phenotypeMolecular MedicineDecember 29, 2025Aravindan Narayanan, Kiran Dhenge, Sharmila A. Bapatstaining Total intracellular cholesterol levels were measured using a colorimetric assay (Abcam, #ab65359) according to the manufacturer’s protocol in 48 h + S and SS samples of A4 and OVMZ6 cells. Free cholesterol

(10) SQLE drives bladder cancer progression by boosting mitochondrial oxidative phosphorylationOncogeneDecember 18, 2025Yihong Dong, Xinjian Jiang, Xinxin Yang, Jinfeng Zhang, Qiang Fu, Yunfei Zhou, Xun Yang, Yin Fu, Yunjing Hou, Mujiao Li, et al.were used for quantifying cholesterol concentrations, measured with a Cholesterol Quantification Kit (ab65359, Abcam). All experiments were carried out in triplicate. Western blot analysis Western blotting was

(11) Multi-cohort analysis and experimental study on the function of squalene epoxidase as a ferroptosis inhibitor in HCCBMC CancerDecember 13, 2025Xun Yang, Yidi Wu, Qingxin Zhang, Yunfei Zhou, Shuyuan Chang, Chunhui Zhang, Dabin Liuand the concentrations of total cholesterol were measured using the Cholesterol Quantification kit (ab65359, Abcam) according to the manufacturer’s instructions. Absorbance was read at 450 nm. A standard curve

(12) Novel murine model provides insights into early-onset of kidney disease in glycogen storage disease type IbMolecular Genetics and MetabolismNovember 1, 2025Kunal Pratap, Cheol Lee, Lisa Zhang, Hung Dar Chen, Irina Arnaoutova, Brian C. Mansfield, Janice Y. Chouand uric acid were analyzed using the corresponding kits, Cholesterol/Cholesteryl Ester Assay Kit (#ab65359), Triglyceride Assay Kit (#ab65336), L-Lactate Assay Kit (#ab65331), and Uric Acid Assay Kit (#ab65344)

(13) Exposure of mice to environmentally relevant per- and polyfluoroalkyl substances (PFAS) alters the sperm epigenomeCommun BiolOctober 28, 2025Leah Gillespie, Jacinta H. Martin, Amanda L. Anderson, Ilana R. Bernstein, Simone J. Stanger, Natalie A. Trigg, John E. Schjenken, Anne-Louise Gannon, Shanu Parameswaran, Shannon P. Smyth, et al.conducted using a commercially available colorimetric reagent kit (Cholesterol Assay Kit; Abcam, UK; AB65359) as per the manufacturer’s instructions. Bioaccumulation factor (BAF) and estimated daily intake values BAF

(14) APOE4 promotes nigral tau hyperphosphorylation through cholesterol in atherosclerosisCell Death DiscovOctober 21, 2025Shanshan Hu, Xiaojia Peng, Bing Xia, Cihang Gu, Baofei Sun, Min Chang, Jiuyang Ding, Longying Pengcholesterol analysis The total and free cholesterol were analyzed using a cholesterol quantitation kit (ab65359, Abcam, USA) according to the manufacturer’s protocols. The cholesterol contents were calculated according

(15) Linc01271 promotes lipid synthesis and MASLD/MASH progression via miR-149-3p/RAB35 axisCellular and Molecular Life SciencesSeptember 8, 2025Zhaoqing Yin, Caibin Yue, Zhipeng Li, Xiuwen Guo, Guohao Wang, Hao Zhang, Wei Wang, Lan Liuwith a triglyceride assay kit (ab65336; Abcam, Cambridge, MA, USA) and a total cholesterol assay kit (ab65359; Abcam) according to the manufacturer’s instructions. Lipids in the Liver were extracted and measured.

(16) Increased atherosclerosis and expression of inflammarafts in macrophage foam cells in AIBP-deficient micebioRxivAugust 16, 2025Shenglin Li, Nicolaus Nazarenkov, Elena Alekseeva, Juliana M. Navia-Pelaez, Patrick Secrest, Philip L.S.M. Gordts, Sven Heinz, Yury I. Millertriglyceride levels in plasma samples were quantified using a cholesterol/cholesteryl ester assay kit (ab65359, Abcam) and a triglyceride assay kit (ab65336, Abcam), respectively, according to manufacturer’s protocol. Aorta

(17) APOE4 Exacerbates Cerebral Tau Pathology Through Cholesterol‐Induced Degradation of Phosphatase in AtherosclerosisCNS Neuroscience & TherapeuticsAugust 1, 2025Jiuyang Ding, Baofei Sun, Yang Gao, Cihang Gu, Jian Zhang, Li Wang, Jie Zheng, Bing Xia, Xiaolan QiAnalysis The measurement of serum cholesterol was performed using a cholesterol quantitation kit (ab65359, Abcam, USA) according to the manufacturer's protocols. Briefly, serum was acquired and then diluted

(18) MiR-181a-driven downregulation of cholesterol biosynthesis through SREBP2 inhibition suppresses uveal melanoma metastasisJ Exp Clin Cancer ResJuly 19, 2025Rui Wang, Claudia Gilbert, Houda Tahiri, Chun Yang, Solange Landreville, Pierre Hardy(IRCM). Cholesterol measurement Total cholesterol was determined with a commercially available assay kit (Abcam; ab65359). Briefly, after treatment, cells and tissues were harvested and washed with cold PBS. Cholesterol was

(19) Cyclodextrins inhibit TRPV1 and TRPA1 activation-induced nociception via cholesterol depletionJournal of Lipid ResearchJune 16, 2025Andrea Nehr-Majoros, Lajos Karakai, Maja Payrits, Noémi Bencze, Ágnes Kemény, György Sétáló, Rita Börzsei, Csaba Hetényi, Zsuzsanna Helyes, Éva Szőkemeasurement To measure the cholesterol level in the mouse ear or the plantar skin after CD pretreatment ab65359 Abcam Cholesterol/Cholesteryl Ester Quantitation Assay kit was used, according to manufacturer’s protocol.

(20) Unlocking therapeutic potential of amlexanox in MASH with insights into bile acid metabolism and microbiomenpj Gut and LiverJune 16, 2025Wenjing You, Jianfei Ji, Danwan Wen, Chen Wang, Xiaoli Sun, Peng Zhaodetermined using the Total Cholesterol and Cholesterol Ester Colorimetric/Fluorometric Kit (Abcam, Cat. ab65359), following the manufacturer’s instructions. Tissue triglyceride/cholesterol levels were normalized

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Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation from Abcam

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Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation from Abcam

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