Fig 1: Effect of ST ethanolic extract administration on ultraviolet (UV)B-induced expression of matrix metalloproteinases (MMPs), pro-collagen, and tissue inhibitor of metalloproteinase 1 (TIMP1) following UVB-irradiation of skin tissue in hairless HR-1 mice. HR-1 mice were orally administered 100 or 200 mg/kg of ST ethanolic extract for 9 weeks. (A) Expression of MMPs (MMP1, 3, and 9) and pro-collagen A1 was detected by Western blot analysis and quantitative values of protein expression in HR-1 mice. All results were normalized to the normal control and calculated using ImageJ software. (B) The level of expression of TIMP1 was detected using an ELISA kit. Results are presented as the mean ± SEM from three independent experiments. # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. normal control; NC, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. UVB irradiation control; Con.
Fig 2: Loss of TRPV4 reduces tumor growth and cytokine expression known to activate hepatocytes and hepatic stellate cells.(A) Schematic of the orthotopic experiment. (B) Representative images of tumors from WT, Piezo1GFAP KO, or TRPV4-KO mice that were injected with 100,000 KPCY cells in the tail of the pancreas. Organs were collected 20 days after surgery. (C) Quantitative analysis of tumor size. (D–F) Serum levels of IL-6 (D), TIMP1 (E), and SAA (F) were measured by ELISA. (G) Saa2/Saa1 from liver RNA was measured by RT-PCR. Statistical analyses were performed using 1-way ANOVA with Dunnett’s post hoc test. Results are expressed as mean ± SEM.
Fig 3: The TRPV4 antagonist GSK2193874 protects mice from premetastatic niche formation.(A) Graphical illustration of the orthotopic surgery with daily gavage of GSK2193874 (10 mg/kg). (B) Quantitative analysis of tumor weight. (C and D) Serum cytokines levels IL-6 (C) and TIMP1 (D) were measured by ELISA. (E and F) Serum SAA was measured by ELISA (E) and liver expression of Saa1/Saa2 genes (F) by RT-PCR. Statistical analysis was performed using Student’s t test. Results were expressed as mean ± SEM. *P ≤ 0.05; **P ≤ 0.01.
Supplier Page from Abcam for Mouse TIMP1 ELISA Kit