Description
The UltraMarathonRT® (uMRT) cDNA Synthesis & Amplification Kit enables you to see more of the transcriptome in each sample. uMRT is a next-generation reverse transcriptase that synthesizes end-to-end cDNA in a single pass from even the most complex RNA molecules—long, structured, high-GC, or low-abundance.
Conventional retroviral RTs are ‘distributive’ meaning they attach and detach many times from each RNA transcript which often creates truncated, incomplete, or incorrect cDNAs. This is often caused by secondary structures or repeat sequences within the template.
UltraMarathonRT solves this problem. It is a highly engineered group II intron-encoded reverse transcriptase with intrinsic helicase activity. It bypasses the drop-offs, biases, and blind spots common in traditional cDNA synthesis workflows.
Why Choose This Kit:
- Higher Gene Detection & Isoform Sensitivity: Captures more genes and isoforms, including alternative splicing and intron retention events that other kits miss.
- Superior Amplification Accuracy: Uniform gene body coverage improves the accuracy of differential gene expression analysis.
- Designed for Modern RNA-Seq Applications: Perfect for short-read sequencing, biomarker discovery, and therapeutic target validation.