Illumina Ribo-Zero Plus rRNA Depletion Kit (96 Samples) 20037135 from Illumina

Product Specs
ItemIllumina Ribo-Zero Plus rRNA Depletion Kit (96 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20037135
ApplicationsRNA-Seq, rRNA depletion
TypeReagent Kit
Quantity96 Reactions
FormatLiquid
Storage-80°C

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Auxin biosynthesis and signaling drive virulence and plant adaptation in Dickeya dadantiiPLoS PathogJuly 17, 2026Amalia Roca, Saray Santamaría-Hernando, Zulema Udaondo, Juan J. Cabrera, Patricia Godoy, Ana Nogueira, Emilia López-Solanilla, Miguel A. Matillalibrary construction, ribosomal RNA was depleted with Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina; Ref. 20037135), according to the manufacturer's instructions. The resulting libraries were quantified by quantitative PCR

(2) Western Diet-Induced Obesity Modulates the Mammary Fat Pad MicroenvironmentCellsJune 8, 2026Md Manirujjaman, Maria D. Sanchez-Pino, Jasjeet Singh, Farzeen Nafees, Mrityunjoy Biswas, Ramesh Thylur Puttalingaiah, Soroor Heidari, Dorota Wyczechowska, Jone Garai, Diana C. Polania-Villanueva, et al.instructions. Briefly, ribosomal RNA was first depleted from total RNA (100 ng) with Ribo-Zero plus kit (cat# 20037135, Illumina, San Diego, CA, USA). Following purification, the RNA was fragmented, and first and second-strand

(3) Eggerthella lenta evades bacteriophage through reversible megabase-scale inversions of capsular polysaccharide gene clustersbioRxivMay 1, 2026Shenwei Zhang, Colin Buttimer, Kai R. Trepka, Kathy N. Lam, Luis A. Ramirez Hernandez, Paola Soto-Perez, Cecilia Noecker, Paolo Canigiula, Edwin F. Ortega, Jenny Lee, et al.Universal Probe Supermix,Bio-rad,172-5280 Qiagen RNeasy Kit,Qiagen,74104 Ribo-Zero Plus kit,Illumina,20037135 HiFi SMRTbell library kit,Pacific Biosciences,103-381-200 GenElute™ Bacterial Genomic DNA Kit,Merck,NA2100 Qubit™

(4) The BaeSR two-component system activates bamK , a paralog of the essential β-barrel foldase bamA , in Klebsiella pneumoniaemBioApril 8, 2026Kelly M. Storek, Janina Reeder, Dawei Sun, Donghong Yan, Austin K. Murchison, Min Xu, Elizabeth Skippington, Steven T. Rutherfordcatalog# 20020596) in combination with the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina catalog# 20037135). Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), and the average

(5) Assessing transcriptomic responses of Salmonella Infantis in the presence of poultry litterMicrobiology SpectrumApril 7, 2026Ruby Paudel, Dianna Bourassa, Sabin Poudelgenomic DNA, and ribosomal RNA was removed using Ribo Zero Plus rRNA Depletion kit (ILLUMINA, Catalog 20037135). Thus, obtained rRNA-depleted RNA samples were further used for cDNA synthesis and library preparation

(6) The transcriptional regulatory network of the Escherichia coli MG1655 reference strainNucleic Acids ResFebruary 2, 2026Heera Bajpe, Jongoh Shin, Ying Hefner, Richard Szubin, Jaemin Sung, Yuan Yuan, Bernhard O Palssona bioanalyzer. All ribosomal RNA (rRNA) was removed using Illumina Ribo-Zero rRNA removal kit (Cat#20037135) for gram-negative bacteria. Sequencing libraries with an average insert length of ∼300 bp were created

(7) Genome-wide regulon of NtrC reveals genetic regulation under nitrogen limitation in Methylomonas sp. DH-1iScienceJanuary 16, 2026Han S, Park JY, Han YH, Bang I, Mun H, Lee OK, Hyung JS, Na JG, Lee EY, Kim D, et al.KAPA HiFi HotStart ReadyMix,Roche,Cat#KK2602 RNAprotect Bacteria Reagent,Qiagen,Cat#76506 Ribo-Zero Plus rRNA Depletion Kit,Illumina,Cat#20037135 RNAClean XP beads,Beckman Coulter,Cat#A63987 KAPA Stranded RNA-seq Library Preparation Kit,Roche,Cat#KK8401 QIAquick

(8) Prodrug florfenicol amine is activated by intrinsic resistance to target Mycobacterium abscessusNature MicrobiologyOctober 30, 2025Gregory A. Phelps, Sinem Kurt, Alexander R. Jenner, Shelby M. Anderson, Thalina D. Jayasinghe, Elizabeth C. Griffith, Carl W. Thompson, Lei Yang, Basil Wicki, Frederick K. Bright, et al.with Ribo-Zero Plus mRNA Library Prep kit according to the manufacturer’s instructions (Illumina, PN 20037135). Paired-end 150 cycle sequencing was performed on a NovaSeq X Plus (Illumina) at SeqCenter. The resulting

(9) Complete genome sequence of soybean vein necrosis virus (SVNV) from AlabamaMicrobiology Resource AnnouncementsOctober 9, 2025Abdelaal H. A. Shehata, Rachel Livingston, Neha Potnis, Edward J. Sikora, K. M. Martinfollowed by ribosomal RNA depletion using the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Cat: 20037135). Libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and

(10) Disruption of histone acetylation homeostasis reveals multilayered chromatin regulation for transcriptional resiliencyEpigenetics ChromatinOctober 3, 2025Vrinda Venu, Eric M. Small, Cullen Roth, Samantha H. Adikari, Anna Hendrika Cornelia Vlot, Kyle A. Sullivan, Chanaka Roshan Abeyratne, Daniel Jacobson, Shawn R. Starkenburg, Karissa Y. Sanbonmatsu, et al.RNA was depleted from 500 ng total RNA using the Ribo-Zero Plus rRNA Depletion Kit (Illumina, Cat. #20037135). Sequencing libraries were generated using the Illumina NextSeq 500 High Output Kit v2.5 (Cat. #20024908)

(11) Metatranscriptomics uncovers diurnal functional shifts in bacterial transgenes with profound metabolic effectsCell Host & MicrobeJuly 9, 2025Stephany Flores Flores Ramos, Nicole Siguenza, Wuling Zhong, Ipsita Mohanty, Amulya Lingaraju, R. Alexander Richter, Smruthi Karthikeyan, April L. Lukowski, Qiyun Zhu, Wilhan D.G. Nunes, et al.commercial assays PureLink RNA mini kit,Life Technologies,Cat# 12183025 Ribo-Zero kit,Illumina,Cat# 20037135 Ultra Sensitive Mouse insulin ELISA Kit,Crystal Chem,Cat# 90080 ,, Deposited data,Deposited data,Deposited

(12) Phage therapy with nebulized cocktail BX004-A for chronic Pseudomonas aeruginosa infections in cystic fibrosis: a randomized first-in-human trialNat CommunJuly 1, 2025Iddo Weiner, Maya Kahan-Hanum, Nufar Buchstab, Lior Zelcbuch, Sharon Navok, Irit Sherman, Julian Nicenboim, Tim Axelrod, Dikla Berko-Ashur, Maya Olshina, et al.(Agilent), with RIN scores of 8.5 and above. Ribosomal RNA was depleted using Ribo-zero Plus (Illumina, cat#20037135), and sequencing libraires were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB,

(13) Bioenergetic stress potentiates antimicrobial resistance and persistenceNat CommunJune 9, 2025Barry Li, Shivani Srivastava, Mustafa Shaikh, Gautam Mereddy, Madison R. Garcia, Eric N. Chiles, Avi Shah, Boatema Ofori-Anyinam, Ting-Yu Chu, Nicole J. Cheney, et al.Agilent 4200 TapeStation. Ribosomal RNA was depleted using the Ribo-Zero Plus rRNA Depletion Kit (#20037135 Illumina; San Diego, CA). cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library

(14) CDK2 Inhibition Produces a Persistent Population of Polyploid Cancer CellsJCI InsightMay 22, 2025Liliya Tyutyunyk-Massey, Zibo Chen, Xiuxia Liu, Masanori Kawakami, Adam Harned, Yeap Ng, Brian Luke, Samuel C. Okpechi, Blessing Ogunlade, Yair Alfaro-Mora, et al.(rRNA) was removed using biotinylated target-specific oligos with the Ribo-Zero Plus rRNA Depletion Kit (20037135, Illumina). RNA was fragmented and used to generate first strand cDNA using reverse transcriptase and

(15) The Addition of Transcriptomics to the Bead-Enabled Accelerated Monophasic Multi-Omics Method: A Step toward Universal Sample PreparationAnalytical ChemistryNovember 19, 2024Joshua D. Breidenbach, Emilio S. Rivera, Tara Harvey, Abigale S. Mikolitis, Chi Yen Tseng, Claire K. Sanders, Emilia Solomon, Cheryl D. Gleasner, M. Grace Thornhill, Kes A. Luchini, et al.depleted from 100 ng of total RNA using the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Cat. #20037135). RNA was converted to cDNA and adapters and indexes were added onto the ends of the fragments to generate

(16) Bridging the gap: Multi‐omics profiling of brain tissue in Alzheimer's disease and older controls in multi‐ethnic populationsAlzheimer's & DementiaAugust 30, 2024Joseph S. Reddy, Laura Heath, Abby Vander Linden, Mariet Allen, Katia de Paiva Lopes, Fatemeh Seifar, Erming Wang, Yiyi Ma, William L. Poehlman, Zachary S. Quicksall, et al.Stranded Total RNA reagent kit (Cat #20020597) and the Illumina Ribo‐Zero Plus rRNA Depletion kit (Cat #20037135), libraries were prepared according to the manufacturer's instructions with 200 ng of total RNA. The

(17) Cellular dynamics in pig-to-human kidney xenotransplantationMedAugust 9, 2024Wanqing Pan, Weimin Zhang, Binghan Zheng, Brendan R. Camellato, Jeffrey Stern, Ziyan Lin, Alireza Khodadadi-Jamayran, Jacqueline Kim, Philip Sommer, Karen Khalil, et al.was constructed following the Illumina Ribo-Zero Plus rRNA Depletion Kit protocol (protocol number: 20037135). Longitudinal single-cell RNA-seq of the recipient’s PBMCs were prepared at the same time after collecting

(18) Multi-omics analysis reveals the dynamic interplay between Vero host chromatin structure and function during vaccinia virus infectionCommunications BiologyJune 11, 2024Vrinda Venu, Cullen Roth, Samantha H. Adikari, Eric M. Small, Shawn R. Starkenburg, Karissa Y. Sanbonmatsu, Christina R. Steadmandepleted from 500 ng total RNA using the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Cat. # 20037135). RNA was converted to cDNA, and adapters and indexes were added onto the ends of the fragments to generate

(19) Radiotolerance of N-cycle bacteria and their transcriptomic response to low-dose space-analogue ionizing irradiationiScienceMay 17, 2024Tom Verbeelen, Celia Alvarez Fernandez, Thanh Huy Nguyen, Surya Gupta, Baptiste Leroy, Ruddy Wattiez, Siegfried E. Vlaeminck, Natalie Leys, Ramon Ganigué, Felice MastroleoAgilent RNA 6000 Nano Kit,Agilent Technologies,Cat#5067-1511 Illumina Ribo-Zero Plus rRNA Depletion Kit,Illumina,Cat#20037135 Illumina TruSeq Stranded Total RNA,Illumina,Cat#20020597 QiAMP DNA Mini kit,Qiagen,Cat#51304 QuantiNova

(20) Rapid dissemination of host metabolism-manipulating genes via integrative and conjugative elementsProc Natl Acad Sci U S AMarch 12, 2024Elena Colombi, Frederic Bertels, Guilhem Doulcier, Ellen McConnell, Tatyana Pichugina, Kee Hoon Sohn, Christina Straub, Honour C. McCann, Paul B. Raineyinstructions. 210 ng total RNA was used for rRNA depletion with the bacterial Ribo-Zero kit (Illumina 20037135), according to the manufacturer’s recommendations. After rRNA depletion, the remaining RNA was fragmented,

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Illumina Ribo-Zero Plus rRNA Depletion Kit (96 Samples) from Illumina

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Illumina Ribo-Zero Plus rRNA Depletion Kit (96 Samples) from Illumina

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