Illumina Ribo-Zero Plus rRNA Depletion Kit (16 Samples) 20040526 from Illumina

Product Specs
ItemIllumina Ribo-Zero Plus rRNA Depletion Kit (16 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20040526
ApplicationsRNA-Seq, rRNA depletion
TypeReagent Kit
Quantity16 Reactions
FormatLiquid
Storage-80°C

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) HyDRA: A pipeline for integrating long- and short-read RNAseq data for custom transcriptome assemblyiScienceJune 19, 2026Isabela Almeida, Xue Lu, Stacey L. Edwards, Juliet D. French, Mainá Bitarkit,Illumina,Cat. # 20020594 Ribo-Zero™ Plus rRNA Depletion Kit,Ribo-Zero™ Plus rRNA Depletion Kit,Illumina,Cat. # 20040526 Nanopore Barcoding Kit 24 V14,Nanopore Barcoding Kit 24 V14,Oxford Nanopore Technologies,Cat. # SQK-NBD114.24 PromethION™

(2) Western Diet-Induced Obesity Modulates the Mammary Fat Pad MicroenvironmentCellsJune 8, 2026Md Manirujjaman, Maria D. Sanchez-Pino, Jasjeet Singh, Farzeen Nafees, Mrityunjoy Biswas, Ramesh Thylur Puttalingaiah, Soroor Heidari, Dorota Wyczechowska, Jone Garai, Diana C. Polania-Villanueva, et al.generated using Illumina Stranded Total RNA prep with Ribo-Zero Plus library preparation kit (cat# 20040526, Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, ribosomal RNA

(3) Live and heat-treated Lactiplantibacillus plantarum induce distinct metabolic and immune responses in intestinal epithelial cellsiScienceMay 15, 2026Kaho Matsumoto, Yuta Takada, Yoshiya Imamura, Hina Yoshida, Kazuhiro Sonomura, Mikako Takahashi, Nobuko Moritoki, Tomoko Shindo, Junya Yamamoto, Leonardo Albarracin, et al.NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530),New England Biolabs,Cat #: E7530 Ribo-Zero Plus rRNA Depletion Kit,Illumina,Cat #: 20040526 NEBNext Ultra II Directional RNA Library Prep Kit,New England Biolabs,Cat #: E7760S RNeasy Mini Kit (50

(4) The Transcriptomic Landscape and Regulatory Signaling Features of Bovine Skeletal Muscle Cells Used for Cultured Meat ProductionFoodsMarch 19, 2026Xing Zhen, Se-Hee Choe, Eun Young Kim, Yingying Mao, Ryoung Eun Kim, Jae-Won Huh, Min Kyu Kim, Jong-Hee Leeprepare cDNA libraries using the TruSeq Stranded Total RNA Kit (Illumina, San Diego, CA, USA, CAT: 20040526) following the manufacturer’s protocol. Libraries were sequenced as 101 bp paired-end reads on an Illumina

(5) Retrotransposon activation during spermatogenesis achieves massive ecDNA biogenesis, but rare integrationGenes & DevelopmentMarch 2, 2026Lauren Tracy, Yao Chen, Zz Zhao ZhangrRNA was depleted enzymatically with the Illumina Ribo-Zero Plus rRNA Depletion kit (Illumina Cat. # 20040526). Then, any residual DNA was removed by TURBO DNase (Invitrogen Cat. # AM2238) treatment for 30 minutes

(6) A bacterial translation activator with an intrinsically disordered RNA-binding regionProceedings of the National Academy of SciencesJanuary 20, 2026Pallabi Basu, Elizabeth A. Farland, James C. Charity, Kade A. Townsend, Simon L. Doveexcept that ribosomal RNAs were depleted using the Ribo-Zero Plus rRNA depletion kit (Illumina Cat. No. 20040526). The final libraries were sequenced by paired-end sequencing using the Illumina NextSeq 2000 P2 XLEAP-SBS

(7) Hfq orchestrates a robust RNA-RNA interaction network in Acinetobacter baumanniimBioJanuary 14, 2026Valerie Intorcia, Mikaela N. Daum, Boyang Cheng, Simon L. Dove, Michael J. Gebhardtadaptations to capture bacterial sRNAs (24). The Ribo-Zero Plus rRNA depletion kit (Illumina, cat. no. 20040526) was used to remove rRNAs. The RIL-seq libraries were sequenced at the Harvard Medical School Biopolymers

(8) IL-27Ra promotes the progression of neonatal necrotizing enterocolitisScientific ReportsNovember 28, 2025Yuling Yang, Yingyan Liu, Limei Zhong, Chun Yan, Xin Zhong, Chaoting Lan, Sitao Li, Yufeng Liumanufacturer’s protocol. Ribosomal RNA was removed with the Ribo-Zero rRNA Removal Kit (Illumina, Cat No. 20040526), and libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, Cat No.

(9) Genetics-nutrition interactions control diurnal enhancer-promoter dynamics and liver lipid metabolismCell MetabolismOctober 7, 2025Dishu Zhou, Ying Chen, Panpan Liu, Kun Zhu, Juliet Holder-Haynes, S. Julie-Ann Lloyd, Cam Mong La, Inna I. Astapova, Seunghee Choa, Ying Xiong, et al.transcription Kit,ThermoFisher Scientific,4368814 Illunima Ribo-Zero Plus rRNA Deleption Kit,Illumina,20040526 TruSeq Standed Total RNA Library Prep Kit,Illumina,20020599 Triglyceride Liquid Reagent for Diagnostic

(10) Bacteria isolated from the grape phyllosphere capable of degrading guaiacol, a main volatile phenol associated with smoke taint in winePLoS OneOctober 1, 2025Claudia Castro, Jacquelyn Badillo, Melissa Tumen-Velasquez, Adam M. Guss, Thomas S. Collins, Frank Harmon, Devin Coleman-Derrsequencing. Ribosomal RNA was removed from total RNA using the Illumina Ribo-Zero PLus rRNA Depletion Kit (20040526, Illumina, San Diego, CA, USA) and cDNA libraries were made using NEBNext Ultra II RNA Library Prep

(11) A novel immunocompetent transgenic mouse model of DHF reveals Syk-mediated Th2-polarized cytokine storm as a key driver of vascular leakageEmerg Microbes InfectJuly 7, 2025YuYa Wang, Yang Yang, YuanSong YuanSong, HuiHui HuiHui, Xiong Xiong, Xi Wu, Nan Nan, ShuNan ShuNan, Zhe Zhe, Chen Ling, et al.library preparation. Initially, ribosomal RNA was depleted using the Ribo-Zero Magnetic kit (Illumina, 20040526), followed by fragmentation of the remaining RNAs. First-stranded cDNAs and double-stranded cDNAs were

(12) Extracellular Vesicles From Xylella fastidiosa Carry sRNAs and Genomic Islands, Suggesting Roles in Recipient CellsJ Extracell VesiclesJune 25, 2025Alessa Ruf, Patrick Blumenkamp, Christina Ludwig, Anne Lippegaus, Andreas Brachmann, Andreas Klingl, Alexander Goesmann, Karina Brinkrolf, Kai Papenfort, Silke RobatzekRNA‐seq of WCL samples, rRNA was depleted using an Illumina Ribo‐Zero Plus rRNA Depletion Kit (Illumina, #20040526). All cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

(13) CDK2 Inhibition Produces a Persistent Population of Polyploid Cancer CellsJCI InsightMay 22, 2025Liliya Tyutyunyk-Massey, Zibo Chen, Xiuxia Liu, Masanori Kawakami, Adam Harned, Yeap Ng, Brian Luke, Samuel C. Okpechi, Blessing Ogunlade, Yair Alfaro-Mora, et al.Kit (73404, Qiagen). The Illumina Stranded Total RNA library preparation ligation with RiboZero plus (20040526, Illumina) was used for RNA-Seq. Ribosomal RNA (rRNA) was removed using biotinylated target-specific

(14) Safeguarding spermatogenesis from retrotransposon insertions by forming ecDNAbioRxivMay 20, 2025Lauren Tracy, ZZ Zhao ZhangrRNA was depleted enzymatically with the Illumina Ribo-Zero Plus rRNA Depletion kit (Illumina Cat. # 20040526). Then, any residual DNA was removed by TURBO DNase (Invitrogen Cat. # AM2238) treatment for 30 minutes

(15) Identification of RBP binding sites using RNA deaminasesMethods in EnzymologyApril 18, 2025Tao Yu, Qishan Liang, Shuhao Xu, Gene W. Yeo15596026)Direct-zol RNA Miniprep Kit (Zymo, R2052)Illumina® Ribo-Zero Plus rRNA Depletion Kit (Illumina, 20040526)TruSeq® Stranded mRNA Library Prep (Illumina, 20020594)RNA ScreenTape (Agilent, 5067–5576)D1000 ScreenTape

(16) Deletion of an sRNA primes development in a multicellular bacteriumiScienceMarch 21, 2025Marco La Fortezza, Jasper Verwilt, Sarah M. Cossey, Sabrina A. Eisner, Gregory J. Velicer, Yuen-Tsu N. Yufor 45 min. Ribosomal RNA (rRNA) was depleted using Ribo-Zero Plus rRNA Depletion Kit (Illumina, # 20040526) according to the manufacturer’s protocol. RNA quality was checked using the Aligent 4150 TapeStation®

(17) Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissuesPloS oneJanuary 1, 2025Li Y, Polychronopoulou M, Boonen I, Fikatas A, Gryseels S, Laudisoit A, de Bellocq JG, Vrancken B, Magiorkinis G, Lemey P, et al.ribosomal RNA (rRNA) depletion step using the Ribo-Zero rRNA Depletion Kit (Human/Mouse/Rat) (catalog #20040526, Illumina, the Netherlands) on an input sample of 1 μg (Fig 1, marked in pink color). Upon depletion,

(18) RNA 5-methylcytosine marks mitochondrial double-stranded RNAs for degradation and cytosolic releaseMolecular CellAugust 8, 2024Sujin Kim, Stephanie Tan, Jayoung Ku, Tria Asri Widowati, Doyeong Ku, Keonyong Lee, Kwontae You, Yoosik KimEpiNext RNA Bisulfite-Seq Kit (Illumina),Epigentek,Cat# P-9006-12 Ribo-Zero Plus rRNA depletion kit,Illumina,Cat# 20040526 TruSeq stranded total RNA library prep,Illumina,Cat# 20020596 Oligo Clean and Concentrator,Zymo Research,Cat#

(19) Microbes translocation from oral cavity to nasopharyngeal carcinoma in patientsNature CommunicationsFebruary 22, 2024Ying Liao, Yan-Xia Wu, Minzhong Tang, Yi-Wei Chen, Jin-Ru Xie, Yan Du, Tong-Min Wang, Yong-Qiao He, Wen-Qiong Xue, Xiao-Hui Zheng, et al.TruSeq® RNA LT Sample Prep Kit v2 (Illumina, Cat #: RS-122) and Ribo-Zero Gold kit (Illumina, Cat #: 20040526), and then sequenced using Illumina NovaSeq 6000 instrument. For microbiota detection, raw reads were

(20) N(1)-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation inductionCell death discoveryMay 12, 2023Qi Z, Zhang C, Jian H, Hou M, Lou Y, Kang Y, Wang W, Lv Y, Shang S, Wang C, et al.RNA. After concentration and quality testing, total RNA was rRNA depleted with Ribo-zero (Illumina, 20040526) and then fragmented using RNA fragmentation reagents (Thermo Scientific, AM8740). Fragmented RNA was

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Illumina Ribo-Zero Plus rRNA Depletion Kit (16 Samples) from Illumina

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Illumina Ribo-Zero Plus rRNA Depletion Kit (16 Samples) from Illumina

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