Description
The NEXTFLEX™ RiboNaut™ rRNA Depletion Kit (Human/Mouse/Rat) removes both cytoplasmic and mitochondrial rRNA from total RNA to free sequencing reads for informative transcripts, including non-polyadenylated RNAs, across human, mouse, and rat samples. The workflow uses subtractive hybridization with biotinylated probes and streptavidin-coated magnetic beads and is optimized for 5 ng to 1 µg total RNA input.The rRNA-depleted RNA proceeds directly into any RNA-seq library preparation, including the NEXTFLEX Rapid Directional RNA-Seq 2.0 kit. Typical applications include total RNA-seq, differential expression, discovery of long noncoding RNAs and other non-poly(A) transcripts, isoform profiling, and studies using low-input or partially degraded RNA from human, mouse, or rat tissues and cell lines. Designed for human, mouse, and rat total RNA samples, it removes both cytoplasmic and mitochondrial ribosomal RNA.Broad transcript detection: Preserves mRNA and non-poly(A) RNA species for total RNA-seq and differential expression studies.Flexible input: From 5 ng to 1 µg total RNA.Straightforward workflow: Subtractive hybridization with biotinylated probes and magnetic-bead capture.Seamless downstream integration: Compatible with essentially any RNA-seq library-prep system, including NEXTFLEX Rapid Directional RNA-Seq 2.0. subsequent libraries can be sequenced on any platform.Throughput ready: Works manually or on common liquid handlers, including Sciclone™ and Zephyr™ workstations