Description
Set of reagents and buffers tailored the straightforward labeling of biomolecules with Tb-Cryptate, as part of the development of time-resolved fluorescence resonance energy transfer (TR-FRET) assays. The Cryptate labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with Terbium cryptate. This kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRF assay development. The N-hydroxysuccinimide activated Terbium-Trisbipyridine (TBP) supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation. The kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices)