from Biorbyt for Human Rab effector MyRIP (MYRIP) ELISA Kit
Application Notes: This Human Rab effector MyRIP (MYRIP) ELISA Kit employs a two-site sandwich ELISA to quantitate MYRIP in samples. An antibody specific for MYRIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYRIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYRIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYRIP bound in the initial step. The color development is stopped and the intensity of the color is measured.