Fig 1: Lesion‒Specific Immunomodulation and Microglial Reprogramming by R‒tFNAs@PB. (A, B) Representative immunofluorescence images and corresponding 2.5D surface plots of IBA‒1 (microglia marker, red), CD86 (M1 marker, green), and CD206 (M2 marker, green) in the hippocampal CA3 region. Nuclei were stained with DAPI (blue). The bottom rows show the fluorescence intensity distribution. (C) Representative Western blot bands of proinflammatory cytokines (IL‒6, TNF‒α, and IL‒1β) and anti‒inflammatory cytokine (IL‒10). (D‒G) Quantification of the Western blot analysis for the indicated cytokines (IL‒6, IL‒1β, TNF‒α, and IL‒10). Data are expressed as Mean±SD (n = 6). Compared with the Control group, **p < 0.01, ***p < 0.001; compared with the EP group, #p < 0.05, ##p < 0.01; ns: no significance. Scale bar = 25 μm
Fig 2: R-tFNAs@PB Modulates Microglial Polarization from a Proinflammatory to an Anti-inflammatory Phenotype In Vitro. (A, B) Immunofluorescence images (A) and quantification (B) of the M2 phenotypic marker CD206 (green). (C, D) Immunofluorescence images (C) and quantification (D) of the M1 phenotypic marker CD86 (green). In (A) and (C), the cytoskeleton was counterstained with Tubulin (red), and nuclei were stained with DAPI (blue). (E‒G) Representative immunofluorescence images (E) showing the expression of iNOS (M1 marker, red) and Arg‒1 (M2 marker, green), along with their corresponding quantitative analysis (F, G). Nuclei were stained with DAPI (blue). (H‒K) qRT‒PCR analysis of mRNA expression levels for pro‒inflammatory cytokines IL‒1β (H), IL‒6 (I), TNF‒α (J), and the anti‒inflammatory cytokine IL‒10 (K) in BV2 cells. Data are expressed as Mean±SD (n = 3). Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Control group; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. LPS group; ns = no significance. Scale bar = 25 μm
Supplier Page from Abcam for Human Mannose Receptor 1 (CD206) ELISA Kit