Description
Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
Principle of the Assay: The Mouse CD36 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD36 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD36 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD36 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD36 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD36 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm