Description
Intended Uses: This 17?MT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit 17?MT. This ELISA kit for research use only!
Principle of the Assay: 17?MT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-17?MT antibody and an 17? MT-HRP conjugate. The assay sample and buffer are incubated together with 17?MT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 17?MT concentration since 17?MT from samples and 17? MT-HRP conjugate compete for the anti-17?MT antibody binding site. Since the number of sites is limited, as more sites are occupied by 17?MT from the sample, fewer sites are left to bind 17?MT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 17?MT concentration in each sample is interpolated from this standard curve