Description
Principle of the Assay: Human Programmed Cell Death 4 (PDCD4) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD4 in samples. An antibody specific for PDCD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDCD4 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PDCD4 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PDCD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: PDCD4 encodes a protein localized to the nucleus in proliferrating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. The decuded 458-amino acid protein has a calculated molecular mass of 50.6kD. PDCD4 is an acidic protein with characteristics of a nuclear nonhistone protein. It contains 2 overlapping GxGxxG nucleotide-binding motifs, which are characteristic of protein kinases, but it does not have a kinase catalytic domain. PDCD4 also has several putative serine and threonine phosphorylation sites