Description
Principle of the Assay: FGF basic, also called FGF 2 (fibroblast growth factor 2) or HBGF 2 (heparin binding Growth factor 2), is the most intensively studied of the 22 mitogenic proteins of the FGF family. Family members share 35-60% amino acid (aa) identity, but only FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 18 kDa FGF.Human bFGF ELISA Kit employs a double antibody sandwich method to quantitate Human bFGF in samples. An antibody specific for Human bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Human bFGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450nm wavelength. The bFGF concentration is proportional to the OD450 nm value