Description
Principle of the Assay: The AAA Biotech' Mouse CCL9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL9 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm:Background/Introduction: Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption