Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to cortisol has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated cortisol and incubated. A competitive inhibition reaction is launched between cortisol (Standards or samples) and Biotin-conjugated cortisol with the pre-coated antibody specific for cortisol. The more amount of cortisol in samples, the less antibody bound by Biotin-conjugated cortisol. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of cortisol in the sample. The color development is stopped and the intensity of the color is measured