Description
Principle of the Assay: The AAA Biotech' Mouse CXCL15 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CXCL15 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse CXCL15 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CXCL15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm:Background/Introduction: Mouse Lungkine/CXCL15, also named WECHE, is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 166 amino acids with a 25 aa predicted signal peptide and a 141 aa mature protein with an extremely long C?terminal tail that protrudes beyond the chemokine fold. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up?regulated under inflammatory conditions. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace. Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation