Description
This experiment aims to count the secreting cells of human IL-31. The kit includes monoclonal antibodies, biotinylated detection antibodies, Streptavidin-ALP and ELISpot plates pre-coated with BCIP/NBT-plus substrates. Human T cell polyclonal activators (mAb CD3-2 and CD28-A) were used as a positive control for cytokine secretion. The pre-coated plate reduces the measurement time and minimizes variability