Description
Principle of the Assay: Testosterone test kit is a solid phase competitive ELISA. The samples, working Testosterone-HRP Conjugate and anti-Testosterone-biotin solution are added to the wells coated with streptavidin. Testosterone in the patient's serum competes with the Testosterone enzyme (HRP) conjugate for binding sites. Unbound Testosterone and testosterone enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Testosterone in the samples. A standard curve is prepared relating color intensity to the concentration of the Testosterone.
Intended Uses: Testosterone ELISA Kit is intended for the quantitative measurement of Testosterone in human serum or plasma