Description
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human VIP antibody. VIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VIP Antibody is added and binds to VIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Vasoactive Intestinal Peptide (also known as VIP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids