Cell Counting Kit-8 (CCK-8) C0005 from TargetMol

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanyTargetMol
Price $79.00Supplier Page View Company Product Page
Catalog NumberC0005
TypeChemical
Quantity10 mL (1000 tests)

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TargetMol

34 Washington Street, Suite 220

Wellesley Hills, MA 02481

United States

Phone: +1-781-999-5354

Email: sales@targetmol.com

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Website: www.targetmol.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangcell-count validation during counting. Cell viability was assessed via Cell Counting Kit-8 (CCK-8) assay (#C0005, TargetMol). At each time point (0, 24, 48, 72, and 96 h), 10 μL of CCK-8 reagent was added to each

(2) FSP1 reduces exogenous coenzyme Q10 and inhibits ferroptosis to alleviate intestinal ischemia–reperfusion injuryJournal of Advanced ResearchJune 1, 2026Tianli Shen, Xingjie Wang, Junxiang Zhang, Yuyao Lin, Lindi Cai, Kai Deng, Cancan Zhou, Guanglin Qiu, Jie Lian, Qinhong Xu, et al.evaluate cell viability in response to varying RSL3 concentrations, 20 µL of CCK-8 solution (TargetMol, C0005) was added to each well after treatment. The plates were then incubated at 37 °C for 2 h. Viability

(3) Bisphenol S drives breast cancer metastasis by inhibiting dopamine receptor D2 to activate the Akt/GSK3β oncogenic axisJournal of Advanced ResearchJune 1, 2026Sicheng Liu, Ping Deng, Chengmeng Liu, Huihui Hong, Qixue Zheng, Jinxian Lin, Jiayi Li, Zhulin Du, Lingling Yang, Kun Luo, et al.0.1, 1 μM) for 24, 48, and 72 h, respectively. Cell viability was assessed using the CCK-8 reagent (C0005, TargetMol, USA). The absorbance was detected at 450 nm (Infinite 200 PRO, Tecan, Austria). Cell migration

(4) Discovery of azaindole/indole-fused pyrimidine tetracyclic scaffolds as novel potent CDK7 inhibitorsJ Enzyme Inhib Med ChemMay 28, 2026Fan Pu, Axiao Tao, Pengxiang Qiu, Weishan Deng, Zijian Chen, Zheng Cao, Yunjiao He, Peng George Wang, Yan Zhang, Chengqing Ning, et al.vehicle control, and cells were cultured for 72 h. Cell Counting Kit-8 (CCK-8) reagent (TargetMol, C0005) was then added to each well, followed by incubation at 37 °C for 1–2 h. Absorbance at 450 nm was measured

(5) Abnormal Sialylation Promotes Chemotherapy Resistance in Bladder Cancer via the PI3K-AKT-mTOR Signaling PathwayCancers (Basel)May 24, 2026Junlong Zhu, Aimin Wang, Hang Tong, Yan Sun, Tinghao Li, Linfeng Wu, Xiaoyu Zhang, Zijia Qin, Weiyang Hemedium was carefully removed, and CCK-8 working solution (TargetMol, Wellesley Hills, MA, USA, Cat. No. C0005) was prepared at a concentration of 10%. A total of 100 μL of working solution was added to each well,

(6) CD8+ T cells in Hashimoto’s thyroiditis-associated papillary thyroid carcinomaEur Thyroid JMay 6, 2026Lirong Wang, Lin Zhang, Jiawen Chen, Dan Wang, Yutong Zhang, Lei Sun, Wenxiu Su, Miao Li, Qi Zhou, Juan Wang, et al.IFN-γ, IL-2, and TNF-α were measured. CCK8 proliferation assay The CCK8 assay kit (TargetMol, USA, C0005) was used to assess cell proliferation according to the manufacturer’s instructions. The inhibition

(7) Cepharanthine inhibits lysosomes and induces apoptosis in triple‑negative breast cancer cellsMol Med RepMay 4, 2026Juan Li, Siqin Zhou, Yu Wang, Guanghan Ou, Danli Yang, Liwen Shen15, 20, 25 and 30 µM) of CEP for 24, 48 or 72 h at 37°C. A 10 µl aliquot of CCK-8 reagent (cat. no. C0005; TargetMol Chemicals Inc.) was then added to each well and the plates were incubated for 1 h at 37°C.

(8) YAP inactivation-mediated autophagy inhibition contributes to cisplatin resistance in ovarian cancer cellsJournal of Ovarian ResearchMay 1, 2026Ngoc Thang Nguyen, Meng-Ru Hsieh, Hieu Dac Hanh Nguyen, Waratchaya Chimphlee, Sarinporn Visitsattapongse, Wen-Tai Chiuconcentrations of cisplatin for 24–48 h. Cell viability was assessed using the CCK-8 assay (TargetMol, #C0005). Absorbance at 450 nm was measured using a microplate reader (BioTek 800 TS). The IC₅₀ values were

(9) Physical activity-associated extracellular vesicles inhibit inflammagen-induced microglial activationBrain, Behavior, & Immunity - HealthMay 1, 2026Chien-Yu Su, Heng-Juei Hsu, Tzu-Feng Wang, Hong-Lin Chou, Sheng-Feng Tsai, Chu-Wan Lee, Ching-Li Cheng, Yang-Kao Wang, Yu-Min Kuodescribed in the experimental design. One hour before the end of treatment, 22 μL of CCK-8 reagent (Cat#: C0005, TargetMol, Boston, MA, USA) was added to each well and incubated for 1 h at 37 °C. Absorbance at 450

(10) Sertoli Cell‐Derived Extracellular Vesicles Orchestrate Cadmium‐Induced Testicular Inflammation and FibrosisAdvanced ScienceMay 1, 2026Jianfeng Ma, Mailin Gan, Shuang Liang, Siyu Chen, Ziling Hao, Yiting Yang, Jiawei Lu, Qihang Wu, Yuqian Shi, Lijun Sun, et al.at different concentrations when reaching 90% confluency. After 24 h of treatment, the CCK‐8 assay (C0005, TargetMol, USA) was used to evaluate the cell viability. Sertoli cells were seeded in 12‐well plates

(11) Tat-HSPE1 suppresses clear cell renal cell carcinoma growth through lysosome-dependent cell deathFront PharmacolMay 1, 2026Lin Zhang, Weiyuan Li, Min Liu, Dong Li, Guang-Zhi JinHB-RF-1000). Critical commercial assay kits involved in this study: Cell Counting Kit-8 (TargetMol, Cat# C0005), One Step TUNEL Apoptosis Assay Kit (Beyotime, Cat# C1089), Nuclear and Cytoplasmic Protein Extraction

(12) ATF3/SLC31A1-Mediated Cuproptosis Contributes to Bortezomib-Induced Peripheral Neurotoxicity and Intervention by (−)-Epigallocatechin GallateInt J Mol SciApril 21, 2026Yonghai Wang, Jiabin Lu, Xuejing Feng, Bo Yang, Qiaojun He, Peihua Luo, Xiaochun Yangidentifier PXD072911. 4.5. CCK-8 The cell inhibition rate was assessed using the CCK-8 (Catalog No. C0005, TargetMol). Following the removal of the existing culture medium, fresh medium containing 10% CCK-8

(13) Modulation of Iron-Induced Glucose Metabolic Reprogramming Alleviates Retinal Pigment Epithelial Cell SenescenceInvest Ophthalmol Vis SciApril 14, 2026Zhenzhen Zhao, Qingjian Ou, Jincheng Zhu, Zixu He, Yike Yang, Jiaming Jiang, Qian Wang, Ye Zhou, Ying Liu, Xiaoman Zhu, et al.statistical analysis. Cell Viability Assay Cell viability was determined using a Cell Counting Kit-8 (C0005; TargetMol Chemicals). The iRPE cells were seeded in 96-well plates at 1.0 × 104 cells per well and

(14) Erianin facilitates pyroptosis in endometrial cancer via targeting m6A reader YTHDF1Chin MedApril 7, 2026Wan Shu, Xing Zhou, Rong Zhao, Kejun Dong, Xiaoyu Shen, Guanxiao Chen, Shuangshuang Cheng, Qi Zhang, Ting Zhou, Jiarui Zhang, et al.(Houston, TX, USA). Cell viability assay Cell viability was assayed using Cell Counting Kit-8 (CCK-8; C0005; TargetMol, Boston, USA). An amount of 5 × 103 cells/well were seeded into 96-well plates and incubated

(15) Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validationFront PharmacolApril 1, 2026Haotian Bai, Heng Zhao, Jinteng Feng, Hongyi Wang, Yixing Li, Zhe Chen, Bin He, Chi Wang, Rui Gao, Rui Zhao, et al.Cell counting Kit-8 (CCK-8) assay Cell viability was evaluated with the Cell Counting Kit-8 (CCK-8, C0005, TargetMol, United States) according to the guidelines of the manufacturer, employing a colorimetric

(16) Immunomodulatory supramolecular hydrogel for rheumatoid arthritis management via adenosine A2A receptor-mediated macrophage remodelingBioactive materialsApril 1, 2026Shi G, Liu X, Wei Y, Gao R, Gao X, Zhou Y, Chen C, Yang J, Wang W, Sun L, et al.vivo biosafety evaluation Cell viability was assessed by utilizing the Cell Counting Kit-8 (CCK-8, C0005, TargetMol, Shanghai, China). Following 24, 48, and 72 h incubation of Raw 264.7 with MTX, free PNSH,

(17) Chemotherapy-induced senescent nasopharyngeal carcinoma cells suppress NK cell-mediated antitumor immunity by upregulating EBI3 via mtDNA-cGAS-STING pathwayCell Communication and SignalingMarch 28, 2026Lin Liang, Siyi Liu, Yanling Li, Feng Zeng, Qian He, Zhenxin Wang, Jun Deng, Yanhong Zhou8 h and then screened with puromycin (ST551, Beyotime) after 3 days. CCK-8 assay The CCK-8 assay (C0005, TargetMol) was used to detect cell viability. Briefly, 5.0 × 10³ NPC cells were inoculated into a 96-well

(18) Arginine dependency in omental metastasis of epithelial ovarian cancer reveals a therapeutic vulnerabilityCell Death & DiseaseMarch 24, 2026Jiming Tian, Ting Lei, Yulu Du, Dalin Wang, Haiping Liang, Zeyu Yan, Jun Xu, Wenhao Xue, Shuangbin Wang, Xuehan Bi, et al.48, 72, or 96 h) at 37 °C under 5% CO₂. To assess cell viability, 10 μL of CCK-8 reagent (Cat. No. C0005, TargetMol, Shanghai, China) was added to each well and incubated for 3 h. Absorbance was measured at

(19) Hypoxia-Induced GRP78 Activation Disrupts the Fndc5/Irisin Axis to Accelerate Skeletal Muscle AtrophyCell Stress and ChaperonesMarch 23, 2026Shiqiang Liu, Linyao Xu, Xinru Song, Zhenhao Zhu, Yuxin Zhang, Yumeng Sun, Sharon Nyoja, Qin Wang, Jialin Gao, Lizhuo Wang, et al.HA15 on cell viability was assessed using the Cell Counting Kit-8 (CCK-8; TargetMol, Boston, MA, USA; C0005). Initially, 2.5 × 10⁴ C2C12 murine myoblasts were seeded into a 96-well plate. Upon reaching 70%-80%

(20) Fisetin alleviates lipopolysaccharide-induced mastitis by inhibiting ferroptosis and modulating the gut microbiotaVet QMarch 17, 2026Chao Tong, Li Wang, Haojie Wen, Xinhui Yao, Jiang Tong, Ruya Zhang, Xiao Li, Yuqiang Xiang, Xuebing Wangpurchased from Solaibro. The Cell Counting Kit-8 (CCK-8) was purchased from TargetMol Chemicals Inc. (C0005), while Trizol (R401-01), HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (R223-01), and ChamQ Universal SYBR qPCR Master Mix (Q711

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Cell Counting Kit-8 (CCK-8) from TargetMol

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Cell Counting Kit-8 (CCK-8) from TargetMol

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