Cell Counting Kit-8 (CCK-8) C0005 from TargetMol

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanyTargetMol
Price $79.00Supplier Page View Company Product Page
Catalog NumberC0005
TypeChemical
Quantity10 mL (1000 tests)

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TargetMol

34 Washington Street, Suite 220

Wellesley Hills, MA 02481

United States

Phone: +1-781-999-5354

Email: sales@targetmol.com

Company Profile

Website: www.targetmol.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangcell-count validation during counting. Cell viability was assessed via Cell Counting Kit-8 (CCK-8) assay (#C0005, TargetMol). At each time point (0, 24, 48, 72, and 96 h), 10 μL of CCK-8 reagent was added to each

(2) METTL7A Downregulation Drives SLC1A5-Mediated Glutamine Competition to Promote Tumor Proliferation and Suppress CD8+ T Cell Immunity in Gastric CancerAdv Sci (Weinh)August 3, 2026Mingjun Sun, Shuwei Dang, Dazhi Zhou, Lixian Ding, Xun Sun, Wei Suo, Jirui Niu, Qingzhu Yang, Zhicheng Zhang, Yanyan Sun, et al.Assays The proliferative capacity of GC cells was evaluated using Cell Counting Kit‐8 (CCK‐8; TargetMol, C0005) and EdU staining (Beyotime Biotechnology, C0078) assays. The colony formation assay was performed by

(3) 4-octyl itaconate alleviates LPS-induced inflammation in Sertoli cells by inhibiting excessive autophagyRedox BiolJuly 17, 2026Yuan Li, Xianglong Wang, Haijuan Yang, Feng Jiang, Meihua Wang, Yue Wang, Dong Niu, Huaming Xi1.54p). 2.4 Cell viability assay Cell viability was assessed using a Cell Counting Kit-8 (CCK-8, C0005, TargetMol, MA, USA) according to the manufacturer's instructions. TM4 Sertoli cells were seeded into

(4) Aberrant CX3CL1-CX3CR1 Signaling Reprograms Microglial Exosome Secretion via KIFC2 to Drive Cognitive Impairment in Chronic PainInt J Mol SciJuly 15, 2026Chen Hu, Xinlu Zhang, Wei Zhao, Wenjun Ke, Haoxiang Ma, Wenna Sang, Qian Gaointo the Transwell co-culture system. PC12 cell viability was assessed using the Cell Counting Kit-8 (C0005, TargetMol, Boston, MA, USA). 4.7. Exosome Isolation Conditioned media harvested from cells cultured

(5) Hyaluronic Acid Molecular Weight Modulates Chitosan-Gelatin Scaffold Properties and Cancer Cell Organization in 3D CulturePolymers (Basel)July 10, 2026Leimapokpam Romina Chanu, Guo-Chung Dong, Ping-Shan Laiobtained from the Bioresource Collection and Research Center (BCRC, Taiwan). Cell Counting Kit-8 (CCK-8; C0005) was purchased from TargetMol (Boston, MA, USA). 2.2. Scaffold Fabrication Porous 3D scaffolds composed

(6) SMARCA4 deficiency contributes to platinum resistance in non-small cell lung cancer by activating the NF-κB-BIRC2/BIRC3 signaling axisJournal of Thoracic DiseaseJune 30, 2026Yufeng Li, Na Zhou, Lin Zhang, Xingfa Huo, Yuming Zhang, Fangfang Yang, Xuchen Zhang, Chuantao Zhang, Dantong Sun, Helei Houpuromycin (E607054, Sangon Biotech, Shanghai, China), and a Cell Counting Kit-8 (CCK-8) assay kit (C0005, TargetMol, Shanghai, China) were used. CBP (S1215, Selleck, Shanghai, China), BAY 11-7082 (S2913, Selleck,

(7) Baihe Gujin decoction ameliorates sepsis-induced acute lung injury through Nrf2/GPX4-mediated antioxidant defense and PPARα-driven metabolic reprogramming: a multi-omics investigationFrontiers in ImmunologyJune 29, 2026Kaiyuan Zhang, Yuqing Huang, Yuan Wu, Zhitao Yang, Fangyu Luo, Yu Han, Lan Zheng, Lingling Lvfetal bovine serum. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8) assay (cat no. C0005; TargetMol Chemicals Inc., Boston, MA, USA). Briefly, cells were seeded into 96-well plates and allowed

(8) Bisphenol S drives breast cancer metastasis by inhibiting dopamine receptor D2 to activate the Akt/GSK3β oncogenic axisJournal of Advanced ResearchJune 1, 2026Sicheng Liu, Ping Deng, Chengmeng Liu, Huihui Hong, Qixue Zheng, Jinxian Lin, Jiayi Li, Zhulin Du, Lingling Yang, Kun Luo, et al.0.1, 1 μM) for 24, 48, and 72 h, respectively. Cell viability was assessed using the CCK-8 reagent (C0005, TargetMol, USA). The absorbance was detected at 450 nm (Infinite 200 PRO, Tecan, Austria). Cell migration

(9) FSP1 reduces exogenous coenzyme Q10 and inhibits ferroptosis to alleviate intestinal ischemia–reperfusion injuryJournal of Advanced ResearchJune 1, 2026Tianli Shen, Xingjie Wang, Junxiang Zhang, Yuyao Lin, Lindi Cai, Kai Deng, Cancan Zhou, Guanglin Qiu, Jie Lian, Qinhong Xu, et al.evaluate cell viability in response to varying RSL3 concentrations, 20 µL of CCK-8 solution (TargetMol, C0005) was added to each well after treatment. The plates were then incubated at 37 °C for 2 h. Viability

(10) CLN7 suppression induces apoptosis via mTOR-regulated and chaperone-mediated autophagy in myeloid leukemia cellsCell Death & DiseaseMay 30, 2026Miaomiao Wu, Hui Li, Sujun Li, Qianwen Xu, Yijing Liao, Lili Qu, Chunlei Cang, Xingbing Wang37 °C in a humidified incubator containing 5% CO2. Subsequently, 10 μL of CCK8 reagent (TargetMol, C0005) was added to each well, followed by a 2 h incubation at 37 °C. Absorbance was measured at 450 nm with

(11) Discovery of azaindole/indole-fused pyrimidine tetracyclic scaffolds as novel potent CDK7 inhibitorsJ Enzyme Inhib Med ChemMay 28, 2026Fan Pu, Axiao Tao, Pengxiang Qiu, Weishan Deng, Zijian Chen, Zheng Cao, Yunjiao He, Peng George Wang, Yan Zhang, Chengqing Ning, et al.vehicle control, and cells were cultured for 72 h. Cell Counting Kit-8 (CCK-8) reagent (TargetMol, C0005) was then added to each well, followed by incubation at 37 °C for 1–2 h. Absorbance at 450 nm was measured

(12) Abnormal Sialylation Promotes Chemotherapy Resistance in Bladder Cancer via the PI3K-AKT-mTOR Signaling PathwayCancers (Basel)May 24, 2026Junlong Zhu, Aimin Wang, Hang Tong, Yan Sun, Tinghao Li, Linfeng Wu, Xiaoyu Zhang, Zijia Qin, Weiyang Hemedium was carefully removed, and CCK-8 working solution (TargetMol, Wellesley Hills, MA, USA, Cat. No. C0005) was prepared at a concentration of 10%. A total of 100 μL of working solution was added to each well,

(13) CD8+ T cells in Hashimoto’s thyroiditis-associated papillary thyroid carcinomaEur Thyroid JMay 6, 2026Lirong Wang, Lin Zhang, Jiawen Chen, Dan Wang, Yutong Zhang, Lei Sun, Wenxiu Su, Miao Li, Qi Zhou, Juan Wang, et al.IFN-γ, IL-2, and TNF-α were measured. CCK8 proliferation assay The CCK8 assay kit (TargetMol, USA, C0005) was used to assess cell proliferation according to the manufacturer’s instructions. The inhibition

(14) Cepharanthine inhibits lysosomes and induces apoptosis in triple‑negative breast cancer cellsMol Med RepMay 4, 2026Juan Li, Siqin Zhou, Yu Wang, Guanghan Ou, Danli Yang, Liwen Shen15, 20, 25 and 30 µM) of CEP for 24, 48 or 72 h at 37°C. A 10 µl aliquot of CCK-8 reagent (cat. no. C0005; TargetMol Chemicals Inc.) was then added to each well and the plates were incubated for 1 h at 37°C.

(15) YAP inactivation-mediated autophagy inhibition contributes to cisplatin resistance in ovarian cancer cellsJournal of Ovarian ResearchMay 1, 2026Ngoc Thang Nguyen, Meng-Ru Hsieh, Hieu Dac Hanh Nguyen, Waratchaya Chimphlee, Sarinporn Visitsattapongse, Wen-Tai Chiuconcentrations of cisplatin for 24–48 h. Cell viability was assessed using the CCK-8 assay (TargetMol, #C0005). Absorbance at 450 nm was measured using a microplate reader (BioTek 800 TS). The IC₅₀ values were

(16) Sertoli Cell‐Derived Extracellular Vesicles Orchestrate Cadmium‐Induced Testicular Inflammation and FibrosisAdvanced ScienceMay 1, 2026Jianfeng Ma, Mailin Gan, Shuang Liang, Siyu Chen, Ziling Hao, Yiting Yang, Jiawei Lu, Qihang Wu, Yuqian Shi, Lijun Sun, et al.at different concentrations when reaching 90% confluency. After 24 h of treatment, the CCK‐8 assay (C0005, TargetMol, USA) was used to evaluate the cell viability. Sertoli cells were seeded in 12‐well plates

(17) Tat-HSPE1 suppresses clear cell renal cell carcinoma growth through lysosome-dependent cell deathFront PharmacolMay 1, 2026Lin Zhang, Weiyuan Li, Min Liu, Dong Li, Guang-Zhi JinHB-RF-1000). Critical commercial assay kits involved in this study: Cell Counting Kit-8 (TargetMol, Cat# C0005), One Step TUNEL Apoptosis Assay Kit (Beyotime, Cat# C1089), Nuclear and Cytoplasmic Protein Extraction

(18) Physical activity-associated extracellular vesicles inhibit inflammagen-induced microglial activationBrain, Behavior, & Immunity - HealthMay 1, 2026Chien-Yu Su, Heng-Juei Hsu, Tzu-Feng Wang, Hong-Lin Chou, Sheng-Feng Tsai, Chu-Wan Lee, Ching-Li Cheng, Yang-Kao Wang, Yu-Min Kuodescribed in the experimental design. One hour before the end of treatment, 22 μL of CCK-8 reagent (Cat#: C0005, TargetMol, Boston, MA, USA) was added to each well and incubated for 1 h at 37 °C. Absorbance at 450

(19) Chicoric acid targets PYGL to normalize glycogenolysis-driven glycolysis to suppress non-small cell lung cancerCellular & Molecular Biology LettersApril 29, 2026Xingfa Huo, Helei Hou, Chuantao Zhang, Xueqin Duan, Hongwei Lan, Yufeng Li, Na Zhou, Xiaochun Zhang(SRB), and colony formation assays Cell viability was determined by using a Cell Counting Kit-8 (Cat#C0005, TargetMol, USA) according to the manufacturers’ instructions. In particular, 1–2 × 103 cells for PYGL

(20) ATF3/SLC31A1-Mediated Cuproptosis Contributes to Bortezomib-Induced Peripheral Neurotoxicity and Intervention by (−)-Epigallocatechin GallateInt J Mol SciApril 21, 2026Yonghai Wang, Jiabin Lu, Xuejing Feng, Bo Yang, Qiaojun He, Peihua Luo, Xiaochun Yangidentifier PXD072911. 4.5. CCK-8 The cell inhibition rate was assessed using the CCK-8 (Catalog No. C0005, TargetMol). Following the removal of the existing culture medium, fresh medium containing 10% CCK-8

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Cell Counting Kit-8 (CCK-8) from TargetMol

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Cell Counting Kit-8 (CCK-8) from TargetMol

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