Cell Counting Kit-8 (CCK-8) C0005 from TargetMol

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanyTargetMol
Price $79.00Supplier Page View Company Product Page
Catalog NumberC0005
TypeChemical
Quantity10 mL (1000 tests)

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TargetMol

34 Washington Street, Suite 220

Wellesley Hills, MA 02481

United States

Phone: +1-781-999-5354

Email: sales@targetmol.com

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Website: www.targetmol.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangcell-count validation during counting. Cell viability was assessed via Cell Counting Kit-8 (CCK-8) assay (#C0005, TargetMol). At each time point (0, 24, 48, 72, and 96 h), 10 μL of CCK-8 reagent was added to each

(2) Discovery of azaindole/indole-fused pyrimidine tetracyclic scaffolds as novel potent CDK7 inhibitorsJ Enzyme Inhib Med ChemMay 28, 2026Fan Pu, Axiao Tao, Pengxiang Qiu, Weishan Deng, Zijian Chen, Zheng Cao, Yunjiao He, Peng George Wang, Yan Zhang, Chengqing Ning, et al.vehicle control, and cells were cultured for 72 h. Cell Counting Kit-8 (CCK-8) reagent (TargetMol, C0005) was then added to each well, followed by incubation at 37 °C for 1–2 h. Absorbance at 450 nm was measured

(3) Cepharanthine inhibits lysosomes and induces apoptosis in triple‑negative breast cancer cellsMol Med RepMay 4, 2026Juan Li, Siqin Zhou, Yu Wang, Guanghan Ou, Danli Yang, Liwen Shen15, 20, 25 and 30 µM) of CEP for 24, 48 or 72 h at 37°C. A 10 µl aliquot of CCK-8 reagent (cat. no. C0005; TargetMol Chemicals Inc.) was then added to each well and the plates were incubated for 1 h at 37°C.

(4) Physical activity-associated extracellular vesicles inhibit inflammagen-induced microglial activationBrain, Behavior, & Immunity - HealthMay 1, 2026Chien-Yu Su, Heng-Juei Hsu, Tzu-Feng Wang, Hong-Lin Chou, Sheng-Feng Tsai, Chu-Wan Lee, Ching-Li Cheng, Yang-Kao Wang, Yu-Min Kuodescribed in the experimental design. One hour before the end of treatment, 22 μL of CCK-8 reagent (Cat#: C0005, TargetMol, Boston, MA, USA) was added to each well and incubated for 1 h at 37 °C. Absorbance at 450

(5) Tat-HSPE1 suppresses clear cell renal cell carcinoma growth through lysosome-dependent cell deathFront PharmacolMay 1, 2026Lin Zhang, Weiyuan Li, Min Liu, Dong Li, Guang-Zhi JinHB-RF-1000). Critical commercial assay kits involved in this study: Cell Counting Kit-8 (TargetMol, Cat# C0005), One Step TUNEL Apoptosis Assay Kit (Beyotime, Cat# C1089), Nuclear and Cytoplasmic Protein Extraction

(6) ATF3/SLC31A1-Mediated Cuproptosis Contributes to Bortezomib-Induced Peripheral Neurotoxicity and Intervention by (−)-Epigallocatechin GallateInt J Mol SciApril 21, 2026Yonghai Wang, Jiabin Lu, Xuejing Feng, Bo Yang, Qiaojun He, Peihua Luo, Xiaochun Yangidentifier PXD072911. 4.5. CCK-8 The cell inhibition rate was assessed using the CCK-8 (Catalog No. C0005, TargetMol). Following the removal of the existing culture medium, fresh medium containing 10% CCK-8

(7) Modulation of Iron-Induced Glucose Metabolic Reprogramming Alleviates Retinal Pigment Epithelial Cell SenescenceInvest Ophthalmol Vis SciApril 14, 2026Zhenzhen Zhao, Qingjian Ou, Jincheng Zhu, Zixu He, Yike Yang, Jiaming Jiang, Qian Wang, Ye Zhou, Ying Liu, Xiaoman Zhu, et al.statistical analysis. Cell Viability Assay Cell viability was determined using a Cell Counting Kit-8 (C0005; TargetMol Chemicals). The iRPE cells were seeded in 96-well plates at 1.0 × 104 cells per well and

(8) Erianin facilitates pyroptosis in endometrial cancer via targeting m6A reader YTHDF1Chin MedApril 7, 2026Wan Shu, Xing Zhou, Rong Zhao, Kejun Dong, Xiaoyu Shen, Guanxiao Chen, Shuangshuang Cheng, Qi Zhang, Ting Zhou, Jiarui Zhang, et al.(Houston, TX, USA). Cell viability assay Cell viability was assayed using Cell Counting Kit-8 (CCK-8; C0005; TargetMol, Boston, USA). An amount of 5 × 103 cells/well were seeded into 96-well plates and incubated

(9) Immunomodulatory supramolecular hydrogel for rheumatoid arthritis management via adenosine A2A receptor-mediated macrophage remodelingBioactive materialsApril 1, 2026Shi G, Liu X, Wei Y, Gao R, Gao X, Zhou Y, Chen C, Yang J, Wang W, Sun L, et al.vivo biosafety evaluation Cell viability was assessed by utilizing the Cell Counting Kit-8 (CCK-8, C0005, TargetMol, Shanghai, China). Following 24, 48, and 72 h incubation of Raw 264.7 with MTX, free PNSH,

(10) Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validationFront PharmacolApril 1, 2026Haotian Bai, Heng Zhao, Jinteng Feng, Hongyi Wang, Yixing Li, Zhe Chen, Bin He, Chi Wang, Rui Gao, Rui Zhao, et al.Cell counting Kit-8 (CCK-8) assay Cell viability was evaluated with the Cell Counting Kit-8 (CCK-8, C0005, TargetMol, United States) according to the guidelines of the manufacturer, employing a colorimetric

(11) Chemotherapy-induced senescent nasopharyngeal carcinoma cells suppress NK cell-mediated antitumor immunity by upregulating EBI3 via mtDNA-cGAS-STING pathwayCell Communication and SignalingMarch 28, 2026Lin Liang, Siyi Liu, Yanling Li, Feng Zeng, Qian He, Zhenxin Wang, Jun Deng, Yanhong Zhou8 h and then screened with puromycin (ST551, Beyotime) after 3 days. CCK-8 assay The CCK-8 assay (C0005, TargetMol) was used to detect cell viability. Briefly, 5.0 × 10³ NPC cells were inoculated into a 96-well

(12) Arginine dependency in omental metastasis of epithelial ovarian cancer reveals a therapeutic vulnerabilityCell Death & DiseaseMarch 24, 2026Jiming Tian, Ting Lei, Yulu Du, Dalin Wang, Haiping Liang, Zeyu Yan, Jun Xu, Wenhao Xue, Shuangbin Wang, Xuehan Bi, et al.48, 72, or 96 h) at 37 °C under 5% CO₂. To assess cell viability, 10 μL of CCK-8 reagent (Cat. No. C0005, TargetMol, Shanghai, China) was added to each well and incubated for 3 h. Absorbance was measured at

(13) Hypoxia-Induced GRP78 Activation Disrupts the Fndc5/Irisin Axis to Accelerate Skeletal Muscle AtrophyCell Stress and ChaperonesMarch 23, 2026Shiqiang Liu, Linyao Xu, Xinru Song, Zhenhao Zhu, Yuxin Zhang, Yumeng Sun, Sharon Nyoja, Qin Wang, Jialin Gao, Lizhuo Wang, et al.HA15 on cell viability was assessed using the Cell Counting Kit-8 (CCK-8; TargetMol, Boston, MA, USA; C0005). Initially, 2.5 × 10⁴ C2C12 murine myoblasts were seeded into a 96-well plate. Upon reaching 70%-80%

(14) Fisetin alleviates lipopolysaccharide-induced mastitis by inhibiting ferroptosis and modulating the gut microbiotaVet QMarch 17, 2026Chao Tong, Li Wang, Haojie Wen, Xinhui Yao, Jiang Tong, Ruya Zhang, Xiao Li, Yuqiang Xiang, Xuebing Wangpurchased from Solaibro. The Cell Counting Kit-8 (CCK-8) was purchased from TargetMol Chemicals Inc. (C0005), while Trizol (R401-01), HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (R223-01), and ChamQ Universal SYBR qPCR Master Mix (Q711

(15) Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopeniaJournal of NanobiotechnologyMarch 16, 2026Benchi Che, Yongzhi Cui, Zhengsheng Chen, Yanchun Gao, Lei Luo, Kaiwen zheng, Jiashuo Liu, Yu Xiang, Jiaqi Cheng, Yuanyuan Guo, et al.in vitro Mouse BMSCs and MuSCs were used to access the cytotoxicity of miR@DT/iMNV with a CCK-8 kit (C0005, TargetMol, Boston, MA, USA). Different cells were incubated with PBS/iMSC-EV/miR@DT-Lipo/ miR@DT/iMNV

(16) Glutamine Deprivation Triggers Tribbles Homolog 3 Dependent G‐Quadruplex Resolution to Maintain DNA Repair and Tumor SurvivalAdvanced ScienceMarch 7, 2026Qiang Ji, Xuedan Sun, Zhangran Sun, Mengfan Li, Xinyu Cheng, Shuai Tian, Rick F. Thorne, Jinming Li, Guangzhi Liu, Mian Wu, et al.Cell Proliferation Assay Cell proliferation assays were preformed using the Cell counting kit‐8 (Cat#C0005, TargetMol) following the manufacturers’ instructions. Briefly, infected HepG2 cells were seeded in

(17) An integrative analysis reveals the mechanism of plastic stabilizers inducing breast cancerPLoS Comput BiolMarch 6, 2026Xingfa Huo, Xueqin Duan, Xiaojuan Huang, Linyuan Xue, Lantao Zhao, Yufeng Li, Xiaochun Zhang, Na Zhoutime points, the original medium was replaced with a culture medium containing 10% CCK-8 reagent (Cat#C0005, TargetMol, USA). Following a 2-hour incubation period in a humidified incubator at 37°C, the absorbance

(18) Engineering NIR-II carbon dots through aniline extension with graphene and nitrogen enrichment for hepatobiliary theranosticsNature CommunicationsMarch 2, 2026Lijuan Yang, Man Li, Yisheng Peng, Yu Zhou, Junjie Zou, Huanhuan Liu, Wenxiang Zhang, Jie Liu, Peng He, Fan Dai, et al.sterile PBS and treated with 100 μL of fresh culture medium and 10 μL of CCK-8 solution (TargetMol, C0005, USA) for an additional 2 h at 37 °C. Cell viability was assessed by measuring the absorbance at 450

(19) Bifendate inhibits cell PARthanatos by activating the MEK/ERK pathwayBiochemistry and Biophysics ReportsMarch 1, 2026Ao-meng Hu, Meng Chen, Can-can Zhu, Zai-man Zhu, Xiao-hui Xua density of 1.3 × 104 cells/well. After the indicated treatments, 10 μL CCK-8 reagent (TargetMol, #C0005) was added to each well, and incubated for 2 h at 37 °C. The absorbance was then measured at 450 nm,

(20) Mechanistic evidence for dibutyl phthalate as an environmental trigger for inflammatory bowel diseaseiScienceFebruary 20, 2026Hang Yuan, Gang Chen, Xuejun Sun, Junhui Yuindicating optimal affinity. CCK-8 Cell viability was assessed using the Cell Counting Kit-8 (CCK-8; CAT#C0005, TargetMol, USA). Initially, NCM460 cells were plated into 96-well plates at a density of 4,000 cells

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Cell Counting Kit-8 (CCK-8) from TargetMol

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Cell Counting Kit-8 (CCK-8) from TargetMol

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