Description
Chromatin immunoprecipitation (ChIP) is a technique to analyze the association of proteins with specific genomic regions in intact cells. The assay involves protein-DNA cross-linking with formaldehyde, followed by cell lysis and fragmentation of the cross-linked chromatin. The subsequent immunoprecipitation of chromatin is performed with an antibody (user supplied) specific to a target histone modification. Protein A magnetic particles are used to isolate the protein-DNA complexes of interest. The immunoprecipitated DNA is eluted and purified using unique magnetic particles. The eluted DNA is ideal for ChIP-seq library preparation. Additionally, the kit includes a negative (IgG) and a positive (H3K4me3) control antibody and primer pairs amplifying H3K4me3 positive and negative loci