Description
Chromatin immunoprecipitation (ChIP) is a technique to analyze the association of proteins with specific genomic regions in intact cells. This kit provides a robust ChIP protocol suitable for investigation of histones and other proteins such as transcriptional factors associated with chromatin from cells and tissues. The assay involves protein-DNA cross-linking with formaldehyde, followed by cell lysis and fragmentation of the cross-linked chromatin. The subsequent immunoprecipitation of chromatin is performed with an antibody (user supplied) specific to a target protein. Protein A magnetic particles are used to isolate the protein-DNA complexes of interest, after which the immunoprecipitated DNA is eluted. The eluted DNA is ideal for qPCR analysis. The kit contains enough reagents to perform 4 chromatin preparations, 24 chromatin immunoprecipitations and 32 DNA isolations