Description
Enzo Life Sciences ROS-ID® ROS/RNS detection kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three detection probes: NO Detection Reagent (Red, Ex/Em 650/670 nm), Oxidative Stress Detection Reagent (Green, Ex/Em 490/525 nm) for total ROS detection, and Superoxide Detection Reagent (Orange, Ex/Em 550/620 nm). It also includes the positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as the negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite